The interaction of acetylcholine receptors in porcine atrial membranes with three kinds of G proteins

• Published in: Japanese circulation journal Vol. 54; no. 9; pp. 1176 - 1184
• Main Authors: HAGA, T, IKEGATA, T, HAGA, K
• Format: Journal Article
• Language: English
• Published: Kyoto Japanese Circulation Society 01.09.1990
• Subjects: Animals; Biological and medical sciences; Carbachol - metabolism; Ethylmaleimide - pharmacology; Fundamental and applied biological sciences. Psychology; GTP-Binding Proteins - metabolism; Heart; Heart Atria - drug effects; Heart Atria - metabolism; Hot Temperature; Membranes - drug effects; Membranes - metabolism; Myocardium - metabolism; Receptors, Muscarinic - metabolism; Swine; Vertebrates: cardiovascular system; Atrium; Exploration; Receiver; Experimental study; Pig; Binding protein; Vertebrata; Mammalia; Animal; Membrane; Acetylcholine; Muscarinic receptor; Artiodactyla; Ungulata

We developed a simple procedure to detect the interaction of muscarinic receptors in atrial membranes with exogenous GTP-binding proteins (G proteins). The procedure consists of mixing atrial membranes with G proteins in the presence of sodium cholate, diluting the mixture with a salt buffer and the...