Studies on the Physiological Activities of Ricin from Castor Bean. I. Purification and Pyrogenic Activity of Ricin
Ricin, which has a highly lethal toxicity and pyrogenic activity, was obtained in a homogeneous state from seeds of Ricinus communis by the method of Ishiguro, et al., with a little modification. Some physicochemical properties of the purified ricin were examined and the values obtained were as foll...
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| Published in | Eisei kagaku Vol. 20; no. 4; pp. 204 - 208 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | Japanese English |
| Published |
The Pharmaceutical Society of Japan
1974
|
| Online Access | Get full text |
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| Abstract | Ricin, which has a highly lethal toxicity and pyrogenic activity, was obtained in a homogeneous state from seeds of Ricinus communis by the method of Ishiguro, et al., with a little modification. Some physicochemical properties of the purified ricin were examined and the values obtained were as follows : Sedimentation constant, S20, 4.70×10-13 ; isoelectric point obtained by the electrofocusing fractionation, pH 7.30, estimated molecular weight, 53000-54000. Treatment of ricin with 2-mercaptoethanol dissociated it into two subunits with molecular weight of ca. 24000 and 30000. Chemical analysis suggested its sugar content to be 6.7% as hexose, and ricin seemed to be made up of 18 kinds of amino acid, there being no evidence for the presence of an abnormal amino acid (s). Pyrogenic activity of ricin was characterized by a long latent period and duration of fever for a long period of time, the amount of fever increased in proportion to the dose, and dose-activity correlation existed in the range of 0.05-0.2 μg/ml/kg. |
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| AbstractList | Ricin, which has a highly lethal toxicity and pyrogenic activity, was obtained in a homogeneous state from seeds of Ricinus communis by the method of Ishiguro, et al., with a little modification. Some physicochemical properties of the purified ricin were examined and the values obtained were as follows : Sedimentation constant, S20, 4.70×10-13 ; isoelectric point obtained by the electrofocusing fractionation, pH 7.30, estimated molecular weight, 53000-54000. Treatment of ricin with 2-mercaptoethanol dissociated it into two subunits with molecular weight of ca. 24000 and 30000. Chemical analysis suggested its sugar content to be 6.7% as hexose, and ricin seemed to be made up of 18 kinds of amino acid, there being no evidence for the presence of an abnormal amino acid (s). Pyrogenic activity of ricin was characterized by a long latent period and duration of fever for a long period of time, the amount of fever increased in proportion to the dose, and dose-activity correlation existed in the range of 0.05-0.2 μg/ml/kg. |
| Author | MOCHIDA, KENSHU KOJA, NORIKAZU |
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| Copyright | The Pharmaceutical Society of Japan |
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| DOI | 10.1248/jhs1956.20.204 |
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| References | 11) P. Andrews, Biochem. J., 91, 222 (1964). 4) 船津勝,船津軍喜,蛋白質核酸酵素,13,905(1968 6) M. Ishiguro, T. Takahashi, G. Funatsu, K. Hayahsi, M. Funatsu, J. Biochem., 55, 587 (1964 9) 加納晴三郎,川崎浩之進,吉田稔,日薬理誌,62,135(1966). 5) E. T. Eperjessy, G. Bálint, K. Thuransky, Acta Physiol. Hung. Tomus, 33, 177 (1965). 13) 真木俊雄,金子勲,小野崎菊夫,野本明男,浮田忠之進,日本薬学会,第93年会講演要旨集,第III分冊,1973,p.98. 3) T. B. Osborn, L. B. Mendel, J. F. Harris, Am. J. Physiol., 14, 259 (1905). 8) M. Tomita, T. Kurokawa, K. Onozaki, N. Ichiki, T. Osawa, T. Ukita, Experimentia, 28, 84 (1972). 14) S. Olsnes, A. Pihl, Biochemistry, 12, 3121 (1973). M. Ishiguro, G. Funatsu, M. Funatsu, Agr. Biol. Chem., 35, 724, 729 (1971). 15) J. Hache, P. Delort, M. Artigers, P. Duchene-Marullaz, C. R. Soc. Biol, 162, 1500 (1968). 12) 林健志,大場義樹,蛋白質核酸酵素,17,304(1972). 10) 松尾雄志,堀尾武一,蛋白質核酸酵素,12,737(1967). 船津勝,船津軍喜,化学と生物,9,490(1971). 7) E. Waldschmidt-Leitz, L. Keller, Z. Physiol. Chem., 351, 990 (1970). |
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| Title | Studies on the Physiological Activities of Ricin from Castor Bean. I. Purification and Pyrogenic Activity of Ricin |
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