32P-Post-labelling method improvements for aromatic compound-related molecular epidemiology studies

• Published in: Mutagenesis Vol. 22; no. 6; pp. 381 - 385
• Main Authors: Munnia, Armelle, Saletta, Federica, Allione, Alessandra, Piro, Sara, Confortini, Massimo, Matullo, Giuseppe, Peluso, Marco
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.11.2007; Oxford Publishing Limited (England)
• Subjects: Ammonium; Animals; Aromatic compounds; Biological and medical sciences; Carcinogens; Carcinogens - pharmacology; Chromatography, High Pressure Liquid - methods; Chromatography, Thin Layer - methods; Deoxyribonucleic acid; DNA; DNA - analysis; DNA Adducts - drug effects; DNA Damage - drug effects; Epidemiology; Fundamental and applied biological sciences. Psychology; Humans; Hydrolysis; Indicators and Reagents - analysis; Leukocytes - drug effects; Liver - drug effects; Mice; Molecular and cellular biology; Molecular Epidemiology; Molecular genetics; Mutagenesis. Repair; Phosphorus Radioisotopes; Police; Radioactive materials; Radioactivity; Thin layer chromatography; Urea; Molecular epidemiology; Labelling; Aromatic compound; Method; Mutagenesis

The 32P-post-labelling assay has emerged as a major tool for detecting bulky DNA adducts in subjects exposed to carcinogens, especially aromatic compounds. However, the 32P-post-labelling protocol still requires the use of high amounts of radioactivity, i.e. 25–50 μCi per sample, an obstacle that li...