Plant Genome Size Estimation by Flow Cytometry: Inter-laboratory Comparison
Flow cytometry is a convenient and rapid method that has been used extensively for estimation of nuclear genome size in plants. In contrast to general expectations, results obtained in different laboratories showed some striking discrepancies. The aim of this joint experiment was to test the reliabi...
Saved in:
Published in | Annals of botany Vol. 82; no. suppl-1; pp. 17 - 26 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford University Press
01.12.1998
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Flow cytometry is a convenient and rapid method that has been used extensively for estimation of nuclear genome size in plants. In contrast to general expectations, results obtained in different laboratories showed some striking discrepancies. The aim of this joint experiment was to test the reliability and reproducibility of methods. Care was taken to avoid a bias due to the quantity of DNA in the nucleus, the procedure for nuclei isolation or the type of instrument. Nuclear DNA content was estimated in nine plant species representing a typical range of genome size (2C = approx. 0·3–30 pg DNA). Each of the four laboratories involved in this study used a different buffer and/or procedure for nuclei isolation. Two laboratories used arc lamp-based instruments while the other two used laser-based instruments. The results obtained after nuclei staining with propidium iodide (a DNA intercalator) agreed well with those obtained using Feulgen densitometry. On the other hand, results obtained after staining with DAPI (binding preferentially to AT-rich regions) did not agree with those obtained using Feulgen densitometry. Small, but statistically significant, differences were found between data obtained with individual instruments. Differences between the same type of instruments were negligible, while larger differences were observed between lamp- and laser-based instruments. Ratios of fluorescence intensity obtained by laser instruments were higher than those obtained by lamp-based cytometers or by Feulgen densitometry. The results obtained in this study demonstrate that flow cytometry with DNA intercalators is a reliable method for estimation of nuclear genome size in plants. However, the study confirmed an urgent need for an agreement on standards. Given the small but systematic differences between different types of flow cytometers, analysis of very small differences in genome size should be made in the same laboratory and using the same instrument. |
---|---|
AbstractList | Flow cytometry is a convenient and rapid method that has been used extensively for estimation of nuclear genome size in plants. In contrast to general expectations, results obtained in different laboratories showed some striking discrepancies. The aim of this joint experiment was to test the reliability and reproducibility of methods. Care was taken to avoid a bias due to the quantity of DNA in the nucleus, the procedure for nuclei isolation or the type of instrument. Nuclear DNA content was estimated in nine plant species representing a typical range of genome size (2C = approx. 0·3–30 pg DNA). Each of the four laboratories involved in this study used a different buffer and/or procedure for nuclei isolation. Two laboratories used arc lamp-based instruments while the other two used laser-based instruments. The results obtained after nuclei staining with propidium iodide (a DNA intercalator) agreed well with those obtained using Feulgen densitometry. On the other hand, results obtained after staining with DAPI (binding preferentially to AT-rich regions) did not agree with those obtained using Feulgen densitometry. Small, but statistically significant, differences were found between data obtained with individual instruments. Differences between the same type of instruments were negligible, while larger differences were observed between lamp- and laser-based instruments. Ratios of fluorescence intensity obtained by laser instruments were higher than those obtained by lamp-based cytometers or by Feulgen densitometry. The results obtained in this study demonstrate that flow cytometry with DNA intercalators is a reliable method for estimation of nuclear genome size in plants. However, the study confirmed an urgent need for an agreement on standards. Given the small but systematic differences between different types of flow cytometers, analysis of very small differences in genome size should be made in the same laboratory and using the same instrument. |
Author | Lucretti, S. Meister, A. Doležel, J. Greilhuber, J. Nardi, L. Obermayer, R. Lysák, M. A. |
Author_xml | – sequence: 1 givenname: J. surname: Doležel fullname: Doležel, J. organization: De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Olomouc, Czech Republic – sequence: 2 givenname: J. surname: Greilhuber fullname: Greilhuber, J. organization: De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Olomouc, Czech Republic – sequence: 3 givenname: S. surname: Lucretti fullname: Lucretti, S. organization: De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Olomouc, Czech Republic – sequence: 4 givenname: A. surname: Meister fullname: Meister, A. organization: De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Olomouc, Czech Republic – sequence: 5 givenname: M. A. surname: Lysák fullname: Lysák, M. A. organization: De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Olomouc, Czech Republic – sequence: 6 givenname: L. surname: Nardi fullname: Nardi, L. organization: De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Olomouc, Czech Republic – sequence: 7 givenname: R. surname: Obermayer fullname: Obermayer, R. organization: De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Olomouc, Czech Republic |
BookMark | eNpVkFFLwzAUhYNMcJv-h-B7Z9K06bo3KVs3nKi4B9lLuGlT6OySkURc_fVmbAg-3Ydzv8PhG6GBNlohdE_JhJKcPZhjY2y9M19WQ-cmYOQECCWMxldoGD7SaBrnZICGhJE0yhhPbtDIuR0hJOY5HaKn1w60x6XSZq_we_uj8Nz5dg--NRrLHi86842L3ofY236GV9orG3UgjQVvbI8Lsz-AbZ3Rt-i6CSvU3eWO0WYx3xTLaP1SrorHdVTFPImjOuMySWtIOFOQU0hpRiuQtTzNznkNqgJST1UVV3kTUsgkzVTCayopzWM2RrNzbWWNc1Y14mDDYNsLSsTJivhvRQQr4mIlwNEZbp1Xxz8S7KfgGctSsfzYim3x_DZdb0qxZb-cFG87 |
CitedBy_id | crossref_primary_10_1016_j_scienta_2019_108603 crossref_primary_10_1093_bioinformatics_btx637 crossref_primary_10_1007_s10681_017_1934_0 crossref_primary_10_1007_s00035_021_00267_6 crossref_primary_10_1016_j_jspr_2009_08_001 crossref_primary_10_1002_cyto_a_24798 crossref_primary_10_12705_631_4 crossref_primary_10_1007_s00606_009_0201_9 crossref_primary_10_32615_bp_2020_111 crossref_primary_10_1007_s10722_007_9268_8 crossref_primary_10_1093_aob_mcy048 crossref_primary_10_1111_tpj_14349 crossref_primary_10_1007_s10265_017_0987_4 crossref_primary_10_1007_s10681_016_1829_5 crossref_primary_10_1086_696688 crossref_primary_10_1007_s10722_005_2639_0 crossref_primary_10_1186_s12864_018_4956_7 crossref_primary_10_1002_tax_12149 crossref_primary_10_3390_plants11202736 crossref_primary_10_1093_molbev_msac035 crossref_primary_10_1139_gen_2015_0132 crossref_primary_10_2478_s11756_020_00630_8 crossref_primary_10_1007_s40415_018_0449_9 crossref_primary_10_1111_j_1438_8677_1999_tb00780_x crossref_primary_10_1600_036364420X16033962925231 crossref_primary_10_1515_sg_2014_0036 crossref_primary_10_3390_genes12060906 crossref_primary_10_1093_aobpla_pls005 crossref_primary_10_1002_csc2_20542 crossref_primary_10_1002_cyto_a_20628 crossref_primary_10_1007_s10681_018_2257_5 crossref_primary_10_1111_tpj_12054 crossref_primary_10_1640_0002_8444_113_1_43 crossref_primary_10_1093_aob_mcab155 crossref_primary_10_1007_s10577_010_9166_3 crossref_primary_10_1002_ece3_2353 crossref_primary_10_1002_jemt_20613 crossref_primary_10_1093_aob_mcm307 crossref_primary_10_1007_s00299_008_0621_y crossref_primary_10_1111_j_1469_8137_2009_03101_x crossref_primary_10_1371_journal_pone_0198758 crossref_primary_10_1016_j_ppees_2019_03_002 crossref_primary_10_5897_JMPR2021_7162 crossref_primary_10_1093_aob_mcr208 crossref_primary_10_1038_s41467_023_38836_4 crossref_primary_10_1002_ajb2_1369 crossref_primary_10_1007_s11103_011_9804_y crossref_primary_10_1038_s41588_021_00807_0 crossref_primary_10_21248_kochia_v10_59 crossref_primary_10_1007_s00606_017_1414_y crossref_primary_10_1007_s00606_007_0552_z crossref_primary_10_1093_botlinnean_boaa071 crossref_primary_10_1038_nprot_2007_310 crossref_primary_10_1002_cbdv_202201033 crossref_primary_10_1073_pnas_2206808120 crossref_primary_10_1111_jpy_13457 crossref_primary_10_1590_0100_29452018568 crossref_primary_10_1007_s13237_015_0131_6 crossref_primary_10_1007_s00035_022_00290_1 crossref_primary_10_1139_g03_054 crossref_primary_10_1093_botlinnean_box101 crossref_primary_10_1139_g00_070 crossref_primary_10_1007_s11627_019_10049_9 crossref_primary_10_1093_aobpla_plr001 crossref_primary_10_1007_s00606_010_0330_1 crossref_primary_10_1139_gen_2013_0039 crossref_primary_10_1371_journal_pone_0275551 crossref_primary_10_1640_0002_8444_109_3_212 crossref_primary_10_1002_aps3_11442 crossref_primary_10_1007_s00299_008_0539_4 crossref_primary_10_1111_nph_13107 crossref_primary_10_1111_j_1469_8137_2012_04370_x crossref_primary_10_15446_acag_v68n4_75939 crossref_primary_10_1111_nph_13330 crossref_primary_10_3372_wi_48_48205 crossref_primary_10_1515_abcsb_2015_0013 crossref_primary_10_3390_genes12091436 crossref_primary_10_1002_ajb2_1544 crossref_primary_10_3389_fpls_2018_00880 crossref_primary_10_3390_agronomy12092160 crossref_primary_10_1007_s10577_021_09673_2 crossref_primary_10_1111_j_1365_2699_2008_02005_x crossref_primary_10_1080_00087114_2003_10589305 crossref_primary_10_1093_aob_mcac021 crossref_primary_10_1007_s10265_017_0996_3 crossref_primary_10_1093_aob_mcaa084 crossref_primary_10_3389_fpls_2020_536507 crossref_primary_10_1051_bioconf_20213800084 crossref_primary_10_1007_s11240_024_02809_2 crossref_primary_10_1038_s41588_019_0480_1 crossref_primary_10_1139_g02_104 crossref_primary_10_17660_ActaHortic_2018_1223_2 crossref_primary_10_1007_s00299_007_0415_7 crossref_primary_10_1038_s41598_018_26796_5 crossref_primary_10_3390_genes12091347 crossref_primary_10_14746_biorc_2022_68_2 crossref_primary_10_1016_j_sajb_2023_06_005 crossref_primary_10_1007_s00606_008_0075_2 crossref_primary_10_7124_bc_0006E5 crossref_primary_10_1007_s10577_006_1106_x crossref_primary_10_1186_s12870_023_04456_9 crossref_primary_10_1105_tpc_110_082537 crossref_primary_10_3835_plantgenome2011_08_0022 crossref_primary_10_17660_ActaHortic_2018_1200_5 crossref_primary_10_1080_00087114_2006_10797914 crossref_primary_10_3389_fpls_2020_01314 crossref_primary_10_1093_aob_mcz194 crossref_primary_10_1002_aps3_11339 crossref_primary_10_1007_s10722_018_0648_z crossref_primary_10_1093_aobpla_plaa011 crossref_primary_10_1002_ece3_8382 crossref_primary_10_1093_aob_mcl141 crossref_primary_10_1186_s12863_018_0610_2 crossref_primary_10_5586_asbp_902 crossref_primary_10_1080_11263504_2023_2165551 crossref_primary_10_1007_s11033_024_09417_5 crossref_primary_10_1111_j_1095_8339_2010_01054_x crossref_primary_10_1111_pbr_12143 crossref_primary_10_3389_fmars_2019_00760 crossref_primary_10_1002_aps3_11452 crossref_primary_10_1038_nature11543 crossref_primary_10_1371_journal_pone_0265405 crossref_primary_10_1104_pp_010196 crossref_primary_10_1093_molbev_msr112 crossref_primary_10_1111_plb_13508 crossref_primary_10_1104_pp_112_207282 crossref_primary_10_1134_S1022795418040129 crossref_primary_10_1093_aob_mcl150 crossref_primary_10_3390_ijms20174143 crossref_primary_10_1093_aob_mcl273 crossref_primary_10_1007_s00606_018_1524_1 crossref_primary_10_1007_s12224_024_09441_0 crossref_primary_10_1093_aobpla_plac028 crossref_primary_10_1590_1677_941x_abb_2023_0174 crossref_primary_10_1016_j_plantsci_2007_03_002 crossref_primary_10_1007_s00035_024_00312_0 crossref_primary_10_1111_j_1365_294X_2006_02800_x crossref_primary_10_3390_plants10071362 crossref_primary_10_1093_botlinnean_boy079 crossref_primary_10_3390_plants9121687 crossref_primary_10_1111_j_1439_0523_2006_01211_x crossref_primary_10_56093_ijas_v86i11_62930 crossref_primary_10_1080_00087114_2012_752910 crossref_primary_10_1111_gcbb_12676 crossref_primary_10_1007_s00122_017_2948_7 crossref_primary_10_1002_cyto_a_20915 crossref_primary_10_1111_nph_12617 crossref_primary_10_1111_j_1526_100X_2011_00825_x crossref_primary_10_1007_s00606_024_01904_3 crossref_primary_10_3390_genes12040563 crossref_primary_10_3732_ajb_1400248 crossref_primary_10_1007_s10722_023_01811_5 crossref_primary_10_1016_j_scienta_2024_113142 crossref_primary_10_1139_G06_138 crossref_primary_10_1038_s41467_023_36503_2 crossref_primary_10_21930_rcta_vol15_num1_art_396 crossref_primary_10_3389_fpls_2023_1133986 crossref_primary_10_1002_cyto_a_20902 crossref_primary_10_1007_s00412_009_0205_9 crossref_primary_10_1007_s00606_007_0518_1 crossref_primary_10_1093_botlinnean_boy085 crossref_primary_10_1002_cyto_a_20907 crossref_primary_10_1007_s11240_010_9719_3 crossref_primary_10_1111_tpj_13070 crossref_primary_10_3732_ajb_1100539 crossref_primary_10_1007_s00035_018_0204_7 crossref_primary_10_17660_th2022_018 crossref_primary_10_1007_s00122_008_0831_2 crossref_primary_10_1016_j_gene_2019_02_090 crossref_primary_10_1016_j_ympev_2017_11_007 crossref_primary_10_1007_s00606_018_1525_0 crossref_primary_10_1002_cyto_a_24499 crossref_primary_10_1002_ajb2_1413 crossref_primary_10_1590_0001_3765202120201881 crossref_primary_10_1086_703127 crossref_primary_10_1002_cyto_a_24495 crossref_primary_10_1007_s42535_019_00091_8 crossref_primary_10_12705_676_11 crossref_primary_10_12705_676_10 crossref_primary_10_1007_s00606_008_0077_0 crossref_primary_10_1111_j_1469_8137_2011_03753_x crossref_primary_10_1186_s12898_018_0172_1 crossref_primary_10_1007_s00606_021_01770_3 crossref_primary_10_3389_fpls_2019_00676 crossref_primary_10_1002_cyto_a_23729 crossref_primary_10_1016_j_mgene_2017_11_003 crossref_primary_10_1111_jse_12563 crossref_primary_10_1055_s_2001_12900 crossref_primary_10_1111_nph_13471 crossref_primary_10_1093_aob_mcac079 crossref_primary_10_1016_j_aquabot_2018_04_001 crossref_primary_10_1002_cyto_a_20562 crossref_primary_10_1007_s00122_004_1655_3 crossref_primary_10_3390_ijms23073948 crossref_primary_10_1002_0471142956_cy0730s38 crossref_primary_10_1007_s00299_009_0687_1 crossref_primary_10_1111_mec_16809 crossref_primary_10_1016_j_acthis_2009_10_005 crossref_primary_10_1038_sj_hdy_6800950 crossref_primary_10_3390_plants10010169 crossref_primary_10_1093_aob_mcl077 crossref_primary_10_1080_00087114_2000_10589175 crossref_primary_10_1093_botlinnean_boab001 crossref_primary_10_1111_j_1469_8137_2011_03937_x crossref_primary_10_1002_cyto_10030 crossref_primary_10_1016_j_envexpbot_2006_04_006 crossref_primary_10_1080_00087114_2006_10797894 crossref_primary_10_1080_14772000_2020_1729890 crossref_primary_10_1508_cytologia_72_295 crossref_primary_10_1079_IVP2005642 crossref_primary_10_3732_ajb_1400296 crossref_primary_10_3389_fpls_2020_01295 crossref_primary_10_1002_ajb2_1162 crossref_primary_10_1093_aobpla_plz007 crossref_primary_10_17221_11_2022_CJGPB crossref_primary_10_2135_cropsci2004_2168 crossref_primary_10_2179_16_120 crossref_primary_10_17221_61_2011_HORTSCI crossref_primary_10_1104_pp_109_148338 crossref_primary_10_3732_ajb_94_8_1391 crossref_primary_10_1038_s41559_020_1209_3 crossref_primary_10_1080_00087114_2018_1460057 crossref_primary_10_1111_pbi_13917 crossref_primary_10_1002_cyto_a_22202 crossref_primary_10_3390_plants11111481 crossref_primary_10_3390_genes13111980 crossref_primary_10_1139_cjb_2021_0113 crossref_primary_10_3732_ajb_0900206 crossref_primary_10_1007_s10577_023_09728_6 crossref_primary_10_1093_aob_mcy137 crossref_primary_10_5735_085_061_0122 crossref_primary_10_1038_sj_hdy_6800696 crossref_primary_10_1093_genetics_161_4_1661 crossref_primary_10_1093_aobpla_plad016 crossref_primary_10_1186_s12862_015_0425_y crossref_primary_10_1007_s00122_006_0329_8 crossref_primary_10_1038_s41598_019_41474_w crossref_primary_10_21248_kochia_v16_172 crossref_primary_10_1007_s11033_022_07599_4 crossref_primary_10_1111_nph_15465 crossref_primary_10_3732_ajb_1600130 crossref_primary_10_1002_tax_584012 crossref_primary_10_1111_jse_12118 crossref_primary_10_17660_eJHS_2022_020 crossref_primary_10_1006_fgbi_1998_1097 crossref_primary_10_1007_s13127_018_0365_7 crossref_primary_10_1002_cyto_a_20253 crossref_primary_10_21248_kochia_v15_135 crossref_primary_10_1007_s00606_017_1427_6 crossref_primary_10_1093_aob_mcy028 crossref_primary_10_1093_aobpla_pls037 crossref_primary_10_3389_fpls_2020_00622 crossref_primary_10_1007_s00606_007_0564_8 crossref_primary_10_1186_s12870_018_1593_x crossref_primary_10_3732_ajb_1600365 crossref_primary_10_1093_aobpla_ply012 crossref_primary_10_1134_S1067413620040025 crossref_primary_10_1038_s41598_019_50907_5 crossref_primary_10_3390_plants11111465 crossref_primary_10_1007_s00122_018_3234_z crossref_primary_10_1554_03_545 crossref_primary_10_1111_j_1095_8312_2010_01517_x crossref_primary_10_1111_j_1601_5223_2004_01739_x crossref_primary_10_1002_cyto_a_20122 crossref_primary_10_5735_085_061_0103 crossref_primary_10_1111_tpj_13843 crossref_primary_10_1093_aob_mcs297 crossref_primary_10_1007_s00299_006_0162_1 crossref_primary_10_1007_s11240_021_02211_2 crossref_primary_10_1007_s40415_022_00829_w crossref_primary_10_3897_CompCytogen_v12i3_27307 crossref_primary_10_1007_s10722_016_0448_2 crossref_primary_10_2135_cropsci2012_05_0319 crossref_primary_10_1111_nph_13833 crossref_primary_10_1007_s00606_018_1494_3 |
ContentType | Journal Article |
DBID | BSCLL AAYXX CITATION |
DOI | 10.1093/oxfordjournals.aob.a010312 |
DatabaseName | Istex CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Botany |
EISSN | 1095-8290 |
EndPage | 26 |
ExternalDocumentID | 10_1093_oxfordjournals_aob_a010312 ark_67375_HXZ_ZCMQ8LTG_Z |
GroupedDBID | --- --K -DZ -E4 -~X .2P .I3 0R~ 1B1 1TH 1~5 23M 2WC 2~F 4.4 482 48X 4G. 53G 5GY 5VS 5WA 5WD 6J9 7-5 70D 71M 79B A8Z AABJS AABMN AACTN AAEDT AAESY AAIMJ AAIYJ AAJKP AALCJ AALRI AAMDB AAMVS AAOGV AAPQZ AAPXW AAQFI AAQXK AARHZ AASNB AAUQX AAVAP AAVLN AAXTN AAXUO ABBHK ABDBF ABEUO ABIXL ABJNI ABLJU ABMNT ABNKS ABPPZ ABPTD ABQLI ABQTQ ABSAR ABWST ABXSQ ABXVV ABZBJ ACGFO ACGFS ACIWK ACNCT ACPRK ACUFI ACUTJ ADACV ADBBV ADEIU ADEYI ADEZT ADFGL ADFTL ADGKP ADGZP ADHKW ADHZD ADIPN ADMUD ADOCK ADORX ADQLU ADRIX ADRTK ADULT ADVEK ADYVW ADZTZ ADZXQ AEEJZ AEGPL AEGXH AEJOX AEKSI AELWJ AEMDU AENEX AENZO AEPUE AETBJ AETEA AEUPB AEWNT AFDAS AFFZL AFGWE AFIYH AFMIJ AFOFC AFRAH AFXEN AGINJ AGKEF AGQXC AGSYK AHMBA AHXPO AIAGR AIJHB AIKOY AJEEA AKHUL AKRWK AKWXX ALMA_UNASSIGNED_HOLDINGS ALUQC AOIJS APIBT APWMN AQVQM ARIXL ASPBG AVWKF AXUDD AYOIW AZFZN AZQFJ BAYMD BCRHZ BEYMZ BHONS BQDIO BSCLL BSWAC BYORX C1A CAG CASEJ CDBKE COF CS3 CZ4 DAKXR DATOO DFEDG DILTD DM4 DPORF DPPUQ D~K E3Z EBD EBS EDH EE~ EJD EMOBN ESTFP ESX F5P F9B FDB FEDTE FGOYB FHSFR FIRID FLUFQ FOEOM FQBLK GAUVT GJXCC GX1 H5~ HAR HVGLF HW0 HYE HZ~ IHE IOX J21 JAAYA JBMMH JENOY JHFFW JKQEH JLS JLXEF JPM JSODD JST KAQDR KBUDW KOP KQ8 KSI KSN LG5 M-Z M49 N9A NEJ NGC NLBLG NOMLY NU- NVLIB O-L O0~ O9- OAWHX OBOKY ODMLO OHT OJQWA OJZSN OK1 OWPYF OZT P2P PAFKI PB- PEELM PQQKQ Q1. Q5Y R2- R44 RD5 RIG RNI ROL ROX ROZ RPM RPZ RUSNO RW1 RXO RZO SA0 SSZ SV3 TLC TN5 TR2 UHS UPT W8F WH7 WOQ X7H XOL XPP Y6R YAYTL YKOAZ YSK YXANX YZZ ZCG ZKX ZMT ~02 ~91 ~KM AAUAY AAYXX ADQBN ATGXG CITATION IPSME RZF |
ID | FETCH-LOGICAL-c2642-d76b45da463ea91a5171cabdb031296daeca0d8ec2c9fa51a7b17e46d1b11923 |
ISSN | 0305-7364 |
IngestDate | Thu Sep 26 19:29:36 EDT 2024 Sun Mar 31 11:38:53 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | suppl-1 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c2642-d76b45da463ea91a5171cabdb031296daeca0d8ec2c9fa51a7b17e46d1b11923 |
Notes | istex:5734062D062546AC3623498C6F572DE950B58BEF Institute of Experimental Botany, Laboratory of Molecular Cytogenetics and Cytometry, Sokolovská 6, CZ-77200 Olomouc, Czech Republic. dolezel@risc.upol.cz ark:/67375/HXZ-ZCMQ8LTG-Z local:17 |
OpenAccessLink | https://academic.oup.com/aob/article-pdf/82/suppl_1/17/17846935/17.pdf |
PageCount | 10 |
ParticipantIDs | crossref_primary_10_1093_oxfordjournals_aob_a010312 istex_primary_ark_67375_HXZ_ZCMQ8LTG_Z |
PublicationCentury | 1900 |
PublicationDate | 1998-12-01 1998-12-1 |
PublicationDateYYYYMMDD | 1998-12-01 |
PublicationDate_xml | – month: 12 year: 1998 text: 1998-12-01 day: 01 |
PublicationDecade | 1990 |
PublicationTitle | Annals of botany |
PublicationTitleAlternate | Ann Bot |
PublicationYear | 1998 |
Publisher | Oxford University Press |
Publisher_xml | – name: Oxford University Press |
SSID | ssj0002691 |
Score | 1.5907222 |
Snippet | Flow cytometry is a convenient and rapid method that has been used extensively for estimation of nuclear genome size in plants. In contrast to general... |
SourceID | crossref istex |
SourceType | Aggregation Database Publisher |
StartPage | 17 |
SubjectTerms | DAPI Feulgen densitometry flow cytometry nuclear DNA content plant genome size propidium iodide standardization |
Title | Plant Genome Size Estimation by Flow Cytometry: Inter-laboratory Comparison |
URI | https://api.istex.fr/ark:/67375/HXZ-ZCMQ8LTG-Z/fulltext.pdf |
Volume | 82 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLbKxgMvE1excZEfGC-VQ3N1wtuINqqxIqEVqepL5FtERddMXSpYf85-6Y7t3DqoNHiJUjuNkvN9Pj6xzwWhdwMY_yLPJWFeIgloyZBwNoCfgXRZOOCMcr2jO_oaDb8Hp5Nw0uvddLyWViV3xPqvcSX_gyq0Aa46SvYfkG1uCg1wDvjCERCG470w1hWHSp05urhQ_fPZWvWPYcTaYERtV57Mi1_99LqE7nJpUjuZBUBSIa-319OmDmHXTG3TKvOirNWFNneLuVofpulhnNrd_VOn48Azm_9YcUuBtv1sJbQvsHEaOG9aR2pWlwQ5ctqVBxOKt-nFsSWisaPAQJcQ6tss5Y6yChZMOqI3b7saOPY6TLvS1UyJ21GqNrizmp5tfP0fit8mxSrMI1UIXTms4A4zhSy8drqrt_jvzIKNbyJb_tTObjTMhpNpNk1H3-Kz8eds-gDtejQJw3pFqJrtvchWZazftU5sm_gftj_NhhG0qwX-u2PVjB-jvepzBB9Zbj1BPbV4ih5-MqA_Q18MwbAlGNYEwy3BML_GmmC4IdhHfJdeuKXXczQ-OR6nQ1JV3yACjGSPSBrxIJQsiHzFEhi4LnUF45LrF0giyZSAQR0r4Ykkh14Y1S5VQSRd7urPhhdoZ1Es1EuEB9AnYGLNaSIDzuKE8pyzIFehJ9w8iPeRX0sju7Q5VjLrG-FnmzLMQIZZJcN99N4IrvnLNuAO7nvhK_SopflrtFMuV-oNmJwlf2swvwUctYlN |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Plant+Genome+Size+Estimation+by+Flow+Cytometry%3A+Inter-laboratory+Comparison&rft.jtitle=Annals+of+botany&rft.au=Dolez%CC%8Cel%2C+J.&rft.au=Greilhuber%2C+J.&rft.au=Lucretti%2C+S.&rft.au=Meister%2C+A.&rft.date=1998-12-01&rft.pub=Oxford+University+Press&rft.issn=0305-7364&rft.eissn=1095-8290&rft.volume=82&rft.issue=suppl-1&rft.spage=17&rft.epage=26&rft_id=info:doi/10.1093%2Foxfordjournals.aob.a010312&rft.externalDBID=n%2Fa&rft.externalDocID=ark_67375_HXZ_ZCMQ8LTG_Z |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0305-7364&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0305-7364&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0305-7364&client=summon |