Glycosylation patterns of membrane proteins of the jellyfish Cyanea capillata
Integral and membrane-associated proteins extracted from neuron-enriched perirhopalial tissue of the jellyfish Cyanea capillata were probed with a panel of lectins that recognize sugar epitopes of varying complexity. Of the 13 lectins tested, only concanavalin A, jacalin lectin and tomato lectin sta...
Saved in:
Published in | Cell and tissue research Vol. 279; no. 2; p. 315 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Germany
01.02.1995
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Integral and membrane-associated proteins extracted from neuron-enriched perirhopalial tissue of the jellyfish Cyanea capillata were probed with a panel of lectins that recognize sugar epitopes of varying complexity. Of the 13 lectins tested, only concanavalin A, jacalin lectin and tomato lectin stained distinct bands on Western blots, indicating the presence of repeating alpha-1,6-mannoses, terminal Gal-alpha-1,6-GalNAc and repeating beta-1,4-linked GlcNAc, respectively. In whole-mounted perirhopalial tissue, jacalin lectin stained several cell types, including neurons, muscle, cilia and mucus strands. Tomato lectin stained secretory cells intensely, and neurons in a punctate fashion. Concanavalin A stained cytoplasmic epitopes in both ecto- and endodermal cells, and ectodermal secretory cells and the mucus strands emanating from them. With the exception of tomato lectin's sugar epitope, the other sugar epitopes identified in this study are "non-complex". This study suggests that while glycosylation of integral and membrane-associated proteins occurs in Cyanea, the sugars post-translationally linked to these proteins tend to be simple. |
---|---|
AbstractList | Integral and membrane-associated proteins extracted from neuron-enriched perirhopalial tissue of the jellyfish Cyanea capillata were probed with a panel of lectins that recognize sugar epitopes of varying complexity. Of the 13 lectins tested, only concanavalin A, jacalin lectin and tomato lectin stained distinct bands on Western blots, indicating the presence of repeating alpha-1,6-mannoses, terminal Gal-alpha-1,6-GalNAc and repeating beta-1,4-linked GlcNAc, respectively. In whole-mounted perirhopalial tissue, jacalin lectin stained several cell types, including neurons, muscle, cilia and mucus strands. Tomato lectin stained secretory cells intensely, and neurons in a punctate fashion. Concanavalin A stained cytoplasmic epitopes in both ecto- and endodermal cells, and ectodermal secretory cells and the mucus strands emanating from them. With the exception of tomato lectin's sugar epitope, the other sugar epitopes identified in this study are "non-complex". This study suggests that while glycosylation of integral and membrane-associated proteins occurs in Cyanea, the sugars post-translationally linked to these proteins tend to be simple. |
Author | Anderson, P A Schetz, J A |
Author_xml | – sequence: 1 givenname: J A surname: Schetz fullname: Schetz, J A organization: Whitney Laboratory, St. Augustine, FL 32086 – sequence: 2 givenname: P A surname: Anderson fullname: Anderson, P A |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7534653$$D View this record in MEDLINE/PubMed |
BookMark | eNotj81KAzEYRbOo1La6cS_kBUa_5MvkZylFq1Bxo-CuJJmETsn8MImLeXsL7erCOXDgrsmiH_pAyAODJwagnl0EQKaFVguyAgReKSl_b8k65xMAE1KaJVmqGoWscUU-d2n2Q56TLe3Q09GWEqY-0yHSLnRusn2g4zSU0F5gOQZ6CinNsc1Hup3P3lJvxzadC_aO3ESbcri_7ob8vL1-b9-r_dfuY_uyrzyXWKpGMSOM9Doqz6NFMEK7GrmLqDVnEVHVTqgoJKA2YJzVPgoAcBB9wzXfkMdLd_xzXWgO49R2dpoP11v8H40QTmI |
CitedBy_id | crossref_primary_10_1007_BF02577691 crossref_primary_10_1016_j_jbiosc_2009_10_002 crossref_primary_10_1007_BF02484401 crossref_primary_10_1016_j_jchromb_2008_06_040 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1007/bf00318487 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Biology |
ExternalDocumentID | 7534653 |
Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, U.S. Gov't, P.H.S Comparative Study Journal Article |
GrantInformation_xml | – fundername: NIMH NIH HHS grantid: MH15737 |
GroupedDBID | --- -4W -56 -5G -BR -EM -Y2 -~C -~X .55 .86 .GJ .VR 06C 06D 0R~ 0VY 199 1N0 2.D 203 28- 29B 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 36B 3O- 3SX 3V. 4.4 406 408 409 40D 40E 53G 5QI 5RE 5VS 67N 67Z 6NX 78A 7RV 7X7 88A 88E 8AO 8FE 8FH 8FI 8FJ 8TC 8UJ 95- 95. 95~ 96X AAAVM AABHQ AACDK AAEOY AAHBH AAHNG AAIAL AAJBT AAJKR AANXM AANZL AAQLM AARHV AARTL AASML AATNV AATVU AAUYE AAWCG AAYIU AAYQN AAYTO AAYZH ABAKF ABBBX ABBXA ABDZT ABECU ABFTV ABHLI ABHQN ABJNI ABJOX ABKCH ABKTR ABLJU ABMNI ABMQK ABNWP ABOCM ABPLI ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACAOD ACBXY ACDTI ACGFS ACHSB ACHVE ACHXU ACIPQ ACIWK ACKNC ACMDZ ACMLO ACOKC ACOMO ACPRK ACZOJ ADBBV ADHIR ADIMF ADINQ ADKNI ADKPE ADRFC ADTPH ADURQ ADYFF ADYPR ADZKW AEBTG AEFIE AEFQL AEGAL AEGNC AEJHL AEJRE AEKMD AEMSY AENEX AEOHA AEPYU AESKC AETLH AEVLU AEXYK AFBBN AFDYV AFEXP AFGCZ AFKRA AFLOW AFQWF AFWTZ AFZKB AGAYW AGDGC AGGDS AGJBK AGMZJ AGQEE AGQMX AGRTI AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AIAKS AIGIU AIIXL AILAN AITGF AJBLW AJRNO AJZVZ AKMHD ALIPV ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AOCGG ARMRJ ASPBG AVWKF AXYYD AZFZN B-. BA0 BBNVY BBWZM BDATZ BENPR BGNMA BHPHI BKEYQ BPHCQ BVXVI CAG CCPQU CGR COF CS3 CSCUP CUY CVF DDRTE DL5 DNIVK DPUIP EBD EBLON EBS ECM EIF EIOEI EJD EMB EMOBN EN4 EPAXT ESBYG EX3 F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNWQR GQ6 GQ7 GQ8 GXS H13 HCIFZ HF~ HG5 HG6 HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ I09 IAO IFM IHE IHR IHW IJ- IKXTQ INH INR ITC ITM IWAJR IXC IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ KDC KOV KOW KPH L7B LAS LK8 LLZTM M0L M1P M4Y M7P MA- MVM N2Q N9A NAPCQ NB0 NDZJH NPM NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OAM P19 P2P PF0 PQQKQ PROAC PSQYO PT4 PT5 Q2X QOK QOR QOS R4E R89 R9I RHV RIG RNI RNS ROL RPX RRX RSV RZK S16 S1Z S26 S27 S28 S3A S3B SAP SBL SBY SCLPG SDH SDM SHX SISQX SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZN T13 T16 TSG TSK TSV TUC U2A U9L UG4 UKHRP UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WJK WK6 WK8 WOW X7M YLTOR Z45 Z7U Z7W Z82 Z83 Z87 Z8O Z8Q Z8V Z8W Z91 ZGI ZMTXR ZOVNA ZXP ~EX ~KM |
ID | FETCH-LOGICAL-c263t-d719496c8f7c2fa30948b532bf38821f3375b47f46038909ba8cf4000b0fcd282 |
ISSN | 0302-766X |
IngestDate | Wed Oct 16 00:45:59 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c263t-d719496c8f7c2fa30948b532bf38821f3375b47f46038909ba8cf4000b0fcd282 |
PMID | 7534653 |
ParticipantIDs | pubmed_primary_7534653 |
PublicationCentury | 1900 |
PublicationDate | 1995-Feb |
PublicationDateYYYYMMDD | 1995-02-01 |
PublicationDate_xml | – month: 02 year: 1995 text: 1995-Feb |
PublicationDecade | 1990 |
PublicationPlace | Germany |
PublicationPlace_xml | – name: Germany |
PublicationTitle | Cell and tissue research |
PublicationTitleAlternate | Cell Tissue Res |
PublicationYear | 1995 |
SSID | ssj0014669 |
Score | 1.5136184 |
Snippet | Integral and membrane-associated proteins extracted from neuron-enriched perirhopalial tissue of the jellyfish Cyanea capillata were probed with a panel of... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 315 |
SubjectTerms | Animals Carbohydrate Sequence Concanavalin A Epitopes - chemistry Glycosylation Lectins Membrane Glycoproteins - chemistry Molecular Sequence Data Molecular Weight Plant Lectins Protein Processing, Post-Translational Scyphozoa - chemistry Staining and Labeling |
Title | Glycosylation patterns of membrane proteins of the jellyfish Cyanea capillata |
URI | https://www.ncbi.nlm.nih.gov/pubmed/7534653 |
Volume | 279 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1LawIxEMdDbRF6KX1J3-TQm2zRTXazOYq0FaFSqII3SbKbQ_EFetl--k4eu2ttSx-XRRJYdH_ZMTOZmT9CtyoRksaxDHgURQGlTASSpCqINedCEclDZbMtBnFvRPvjaFwpFdrqkrW8U29f1pX8hyqMAVdTJfsHsuVNYQA-A1-4AmG4_orx4zRXi1Xu0tlMi1QT3Vu5I_MZuMFzUwW1MHqWqyIX4DWbTnNt2hh1c5gXTSWWRnhoLTa3qV0T0bO5lZZL07cEKkPHL4DaCsE2-1UwtGPrZNwp_rMfTn2BXVSkIJdmh8AAc_qCpY0MneKLXwzhhsUjrhrzkyV2yRdSW6tB3Z_qBpLlzDIBZ8k0ePtxcqsntp-poRpLjF7HwIRo_NERrDzujo7cz9joSVt9m31U9_fYcifstmJ4iA68P4A7Du4R2snmx6juFELzE_T0ATEuEOOFxgViXCA2g4AYl4ixQ4xLxKdo9HA_7PYCr4ARqDAm6yBlbU55rBLNVKgFAV88kREJpSbgGbU1ISySlGkamz6JLS5FojRY5ZZsaZWCN91Au_PFPDtDOOSsLVPJmJQppSTmRloAto9CRTxjonWOGu45TJauzcnEP6CL7yYu0X61fq7Qnoa3KruGLdpa3lgk77sAOsM |
link.rule.ids | 780 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Glycosylation+patterns+of+membrane+proteins+of+the+jellyfish+Cyanea+capillata&rft.jtitle=Cell+and+tissue+research&rft.au=Schetz%2C+J+A&rft.au=Anderson%2C+P+A&rft.date=1995-02-01&rft.issn=0302-766X&rft.volume=279&rft.issue=2&rft.spage=315&rft_id=info:doi/10.1007%2Fbf00318487&rft_id=info%3Apmid%2F7534653&rft_id=info%3Apmid%2F7534653&rft.externalDocID=7534653 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0302-766X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0302-766X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0302-766X&client=summon |