Protective Effect of Genistein on Lipopolysaccharide-induced Acute Lung Injury in Rats

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 25; no. 4; pp. 454 - 457
• Main Author: 李兴旺 徐涛 连庆泉 曾邦雄 张冰 谢玉波
• Format: Journal Article
• Language: English
• Published: China Department of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical College, Wenzhou , 325027, China%Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2005
• Subjects: 45-三羟（基）异黄酮; Animals; Genistein - pharmacology; Genistein - therapeutic use; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - genetics; Lipopolysaccharides; Lung - metabolism; Lung - pathology; Male; Random Allocation; Rats; Rats, Sprague-Dawley; Respiratory Distress Syndrome, Adult - chemically induced; Respiratory Distress Syndrome, Adult - prevention & control; RNA, Messenger - biosynthesis; 保护作用; 动物实验; 急性肺损伤; 脂多糖; ICAM-1; lipopolysaccharide; acute lung injury; neutrophils
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/BF02828222

Summary： To investigate the protective effect of genistein on endotoxin-induced acute lung injury in rats, and explore the underlying mechanisms, 32 male Sprague-Dawley rats were randomly divided into 4 experimental groups： saline control, genistein alone, lipopolysaccaride alone, and genistein pretreatment. Each treatment group consisted of eight animals. Animals were observed for 6 h after LPS challenge, and the wet/dry （W/D） weight ratio of the lung and bronchoalveolar lavage fluid （BALF） protein content were used as a measure of lung injury. Neutrophil recruitment and activation were evaluated by BALF cellularity and myeloperoxidase （MP（）） activity. RT-PCR analysis was performed in lung tissue to assess gene expression of ICAM-1. The histopathological changes were also observed using the HE staining of lung tissue. Our results showed that lung injury parameters, including the wet/dry weight ratio and protein content in BALF, were significantly higher in the LPS alone group than in the saline control group （P〈0.01）. In the LPS alone group, a larger number of neutrophils and greater MPO activity in cell-free BAL and lung homogenates were observed when compared with the saline control group （P〈0.01）. There was a significant increase in lung ICAM-1 mRNA in response to LPS challenge （P〈0. 01, group L versus group S）. Genistein pretreatment significantly attenuated LPS-induced changes in these indices. LPS caused extensive lung damage, which was also lessened after genistein pretreatment. All above-mentioned parameters in the genistein alone group were not significantly different from those of the saline control group. It is concluded that genistein pretreatment attenuated LPS-induced lung injury in rats. This beneficial effect of genistein may involves, in part, an inhibition of neutrophilic recruitment and activity, possibly through an inhibition of lung ICAM-1 expression.