Derivation of Macrophages from Mouse Bone Marrow

Macrophages are cellular components of the immune system that are essential for responding to pathogens, initiating inflammation and maintaining tissue homeostasis. Isolation, culture, and functional characterization of bone marrow-derived macrophages from mice are exceptionally powerful in vitro te...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1960; p. 41
Main Author Davis, Beckley K
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Online AccessGet more information

Cover

Loading…
Abstract Macrophages are cellular components of the immune system that are essential for responding to pathogens, initiating inflammation and maintaining tissue homeostasis. Isolation, culture, and functional characterization of bone marrow-derived macrophages from mice are exceptionally powerful in vitro techniques used to examine aspects of macrophage biology, including effector functions, such as phagocytosis, cytokine secretion, oxidative burst, migration, and antigen processing and presentation. These studies can be carried out using wild-type, gene-ablated, and/or transgenic mice. The quantity, purity, and ease of culture of these cells enhance their utility for primary cell cultures to understand macrophage biology. Mouse macrophages have become a cognate animal model for the study of human macrophage biology and disease. This chapter outlines protocols used to generate, polarize, quantitate, and functionally evaluate macrophages derived from bone marrow precursor cells.
AbstractList Macrophages are cellular components of the immune system that are essential for responding to pathogens, initiating inflammation and maintaining tissue homeostasis. Isolation, culture, and functional characterization of bone marrow-derived macrophages from mice are exceptionally powerful in vitro techniques used to examine aspects of macrophage biology, including effector functions, such as phagocytosis, cytokine secretion, oxidative burst, migration, and antigen processing and presentation. These studies can be carried out using wild-type, gene-ablated, and/or transgenic mice. The quantity, purity, and ease of culture of these cells enhance their utility for primary cell cultures to understand macrophage biology. Mouse macrophages have become a cognate animal model for the study of human macrophage biology and disease. This chapter outlines protocols used to generate, polarize, quantitate, and functionally evaluate macrophages derived from bone marrow precursor cells.
Author Davis, Beckley K
Author_xml – sequence: 1
  givenname: Beckley K
  surname: Davis
  fullname: Davis, Beckley K
  email: beckley.davis@fandm.edu
  organization: Department of Biology, Franklin & Marshall College, Lancaster, PA, USA. beckley.davis@fandm.edu
BackLink https://www.ncbi.nlm.nih.gov/pubmed/30798519$$D View this record in MEDLINE/PubMed
BookMark eNo1jttKxDAURYMozkW_QJD-QJxzcu151HHUgRl80eeSJqlWbFNSR_HvLahPG_aCvfaCHfepj4xdIFwhgF2RLTlyRZI4obGcKnnE5kgKuAFBM7YYxzcAZaVQp2wmwVKpkeYMbmNuP91Hm_oiNcXe-ZyGV_cSx6LJqSv26TDG4mayTSzn9HXGThr3Psbzv1yy57vN0_qB7x7vt-vrHffCoOTOBesN6VrLRusYgtdChhrRq6hK7YIySgjlhG0AvRQUKcBUlR5JQgliyS5_d4dD3cVQDbntXP6u_q-LHzF4RJ0
CitedBy_id crossref_primary_10_1016_j_aca_2023_341434
crossref_primary_10_3390_diagnostics13101724
crossref_primary_10_3389_fimmu_2019_02714
crossref_primary_10_3389_fimmu_2023_1190261
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
DOI 10.1007/978-1-4939-9167-9_3
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
DatabaseTitleList MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Biology
EISSN 1940-6029
ExternalDocumentID 30798519
Genre Journal Article
GroupedDBID ---
29M
53G
ACGFS
ALMA_UNASSIGNED_HOLDINGS
CGR
CUY
CVF
ECM
EIF
F5P
NPM
P2P
RSU
SPO
UDS
WH7
ZGI
ID FETCH-LOGICAL-c2613-aad7c695b53f55eddc523db11c4e485ad464224a27f01c329e9d06428c1930802
IngestDate Wed Oct 16 00:49:57 EDT 2024
IsPeerReviewed false
IsScholarly true
Keywords Flow cytometry
Cytokine
Bone marrow
Inflammation
Phagocytosis
ELISA
Macrophage
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c2613-aad7c695b53f55eddc523db11c4e485ad464224a27f01c329e9d06428c1930802
PMID 30798519
ParticipantIDs pubmed_primary_30798519
PublicationCentury 2000
PublicationDate 2019-00-00
PublicationDateYYYYMMDD 2019-01-01
PublicationDate_xml – year: 2019
  text: 2019-00-00
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Methods in molecular biology (Clifton, N.J.)
PublicationTitleAlternate Methods Mol Biol
PublicationYear 2019
SSID ssj0047324
Score 2.290471
Snippet Macrophages are cellular components of the immune system that are essential for responding to pathogens, initiating inflammation and maintaining tissue...
SourceID pubmed
SourceType Index Database
StartPage 41
SubjectTerms Animals
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Inflammation - immunology
Inflammation - metabolism
Macrophages - cytology
Macrophages - metabolism
Mice
Mice, Transgenic
Phagocytosis - physiology
Respiratory Burst - physiology
Title Derivation of Macrophages from Mouse Bone Marrow
URI https://www.ncbi.nlm.nih.gov/pubmed/30798519
Volume 1960
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JasMwEBVNS0supftedOgtONiyZEfHpgsh4JwSyC3IWuglyyEU2q_vSPJG2tLlYoIV5EQvGb8Z671B6I6HJkol5YHgXAU0ESQQTOaBEUB_iYY7ILHi5GyUDCZ0OGXTuj-hU5es8658_1JX8h9U4RzgalWyf0C2mhROwGvAF46AMBx_hfEjXOS14nyZsO24XoR1bXCqkQyyet3pLxe2v5D1Wmwy0cy1jna7Yedli9xOacnkH-2adanGGnYbFYPKmKCv3XPholJalA6sWqkqHWgf7rjd_B8WM5TxkHuH_yKkeV-qT5G23lwBOSiPOURNCLl8FjffDcu1mrvFh1DCgdzxn0c37K_LoRZqpT3bm2NkyzH-VktToIOVlZR3C974NG20V86wkTg4AjE-QPsF88f3HsZDtKUXR2jX9wJ9O0ZhDSZeGtwAE1swsQMTWzCxB_METZ6fxg-DoGhnEUhIU-NACJXKhLOcxYYxrZRkJFZ5FEmqaY8JRSEXJFSQ1ISRjAnXXLn0UALJtpLoU7S9gKucI2wVwVxGOaVKUEg4e0wpkzIgPJqoROQX6Mx_1dnKe5bMykW4_HbkCrXr38g12jHwJ9E3wLjW-a1b9Q81kyR2
link.rule.ids 780
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Derivation+of+Macrophages+from+Mouse+Bone+Marrow&rft.jtitle=Methods+in+molecular+biology+%28Clifton%2C+N.J.%29&rft.au=Davis%2C+Beckley+K&rft.date=2019-01-01&rft.eissn=1940-6029&rft.volume=1960&rft.spage=41&rft_id=info:doi/10.1007%2F978-1-4939-9167-9_3&rft_id=info%3Apmid%2F30798519&rft_id=info%3Apmid%2F30798519&rft.externalDocID=30798519