Sex‐Based Differences in Thrombus Composition in Acute Ischemic Stroke

• Published in: Stroke: vascular and interventional neurology Vol. 5; no. 2
• Main Authors: Schwarz, Ghil, Cascio Rizzo, Angelo, Cervo, Amedeo, Di Como, Martina, Costanza Aquilano, Maria, Valtorta, Emanuele, Carlo Pero, Guglielmo, Piano, Mariangela, Sessa, Maria
• Format: Journal Article
• Language: English
• Published: Phoenix Wiley Subscription Services, Inc 01.03.2025; Wiley
• Subjects: acute ischemic stroke; Blood clots; endovascular thrombectomy; Ischemia; Multivariate analysis; Neurology; Sexes; sex‐based differences; Stroke; thrombus composition
• ISSN: 2694-5746; 2694-5746
• DOI: 10.1161/SVIN.124.001617

BackgroundEndovascular thrombectomy allows for detailed study of thrombus composition in acute ischemic stroke. Differences in thrombus composition can affect outcomes, and the impact of sex on coagulation physiology is recognized. This study investigates whether thrombus composition in acute ischemic stroke varies between sexes.MethodsRetrospective study on consecutive endovascular thrombectomy‐treated patients with acute ischemic stroke at Niguarda Hospital in 2023. Thrombi were stained with hematoxylin and eosin, and the percentages of fibrin, red blood cells (RBCs), and white blood cells (WBCs) were quantified as proportions of the total thrombus area. Univariate and multivariate analyses were performed to identify histological differences between sexes—multivariate analysis adjusted for variables associated with each thrombus component with a P value <0.1.ResultsSeventy‐three cases (50.7% female) were included in the study. Male patients exhibited a higher percentage of RBCs within the thrombus (median 51.5% versus 33.8%, P = 0.004), whereas female patients had a higher percentage of fibrin (median 58.3% versus 45.1%, P = 0.020) and WBCs (median 6.5% versus 4.4%, P = 0.045). Univariate analysis indicated that sex was associated with all 3 thrombus components: fibrin (β = 10.08 [95% CI, 1.29–18.87], P = 0.025), RBCs (β = −12.12 [95% CI, l−21.04 to −3.21], P = 0.008), and WBCs (β = 2.05 [95% CI, 0.23–3.86], P = 0.028). In the multivariate analysis, sex lost its association with both fibrin and RBC percentages, and only baseline RBC count maintained its independent association with these 2 components. For the percentage of WBCs in the thrombus, both sex (β = 2.13 [95% CI, 0.25–4.01], P = 0.027) and stroke etiology (β = 0.81 [95% CI, 0.03–1.58], P = 0.041) showed significant and independent associations.ConclusionOur exploratory study shows that sex independently affects the percentage of WBCs in thrombi. The percentage of fibrin and RBC does not have an independent association with sex and may be mediated by different RBC count. Future research in this field should consider sex and baseline laboratory values.