Polystyrene microplastic interaction with Oryza sativa : toxicity and metabolic mechanism

• Published in: Environmental science. Nano Vol. 8; no. 12; pp. 3699 - 3710
• Main Authors: Wu, Jiani, Liu, Weitao, Zeb, Aurang, Lian, Jiapan, Sun, Yuebing, Sun, Hongwen
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 09.12.2021
• Subjects: Agricultural ecosystems; Agricultural land; Amino acids; Aquatic plants; Ecosystems; Endocytosis; Hydroponics; Internalization; Metabolic pathways; Metabolic response; Metabolism; Metabolomics; Microplastics; Nutrient uptake; Nutritional requirements; Oxidative stress; Phytotoxicity; Plastic pollution; Polystyrene; Polystyrene resins; Presenilin 1; Rice; Risk assessment; Roots; Seedlings; Soil; Terrestrial ecosystems; Toxicity; Uptake
• ISSN: 2051-8153; 2051-8161
• DOI: 10.1039/D1EN00636C

Little is known about the effects of microplastics on terrestrial ecosystems, especially agricultural soils and terrestrial higher plants. Here, rice seedlings were exposed to two different-sized polystyrene (PS) microplastics (100 nm and 1 μm) at 0, 0.1, 1 and 10 mg L −1 under hydroponic conditions for 2 weeks to investigate the metabolic mechanism of PS-induced phytotoxicity and the internalization of PS in rice. PS can trigger oxidative stress. Metabolomic analysis showed that rice leaves had a stronger metabolic response than roots, and the influence of PS on rice metabolic profile was dose-dependent. Metabolic pathways, especially amino acid metabolism, were regulated to affect the growth and development of rice. Phenotypic indexes are consistent with the results of metabolomics. The primary root length of rice was decreased and the nutrient uptake was inhibited, while lateral roots were stimulated to grow to meet the nutritional requirement. Moreover, we found that PS 100 nm rather than PS 1 μm can be effectively accumulated in rice roots through endocytosis, although PS 1 μm exhibited stronger phytotoxicity than PS 100 nm . Our study provides direct evidence for the negative effects of PS on rice, which may have significant implications for assessing the risk of microplastics to terrestrial ecosystems.