Flower color modifications of Torenia hybrida by cosuppression of anthocyanin biosynthesis genes

• Published in: Molecular breeding Vol. 6; no. 3; pp. 239 - 246
• Main Authors: Suzuki, Ken-ichi, Hui-min, Xue, Tanaka, Yoshikazu, Fukui, Yuko, Fukuchi-Mizutani, Masako, Murakami, Yasuyuki, Katsumoto, Yukihisa, Tsuda, Shinzo, Kusumi, Takaaki
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01.06.2000
• Subjects: Anthocyanins; Biosynthesis; Breeding; Carotenoids; Chalcone synthase; Color; Cultivars; dihydroflavanol 4-reductase; Flavonoids; Flowers; Gene expression; Genes; Hydroxylase; Lobes; Molecular biology; Plant biology; Reductase; Reductases; Sterility; Torenia; Torenia hybrida
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1023/A:1009678514695

White and blue/white varieties of Torenia hybrida were successfully obtained from the blue variety cv. Summerwave (SWB) by cosuppressing expression of two of the genes involved in anthocyanin biosynthesis; chalcone synthase (CHS) and dihydroflavonol 4-reductase (DFR). Such molecular breeding is the only precise and efficient way to create flower color variation in SWB due to its male and female sterility. Flower color and the degree of suppression varied between transgenic lines, and anthocyanin biosynthesis was more consistently suppressed in the dorsal petal lobes, ventral petal lobes and corolla tube than lateral petal lobes. A pink variety was obtained by cosuppressing the flavonoid 3′,5′-hydroxylase (F3′5′H) gene. Yellow torenia was obtained from T-33, an in-house cultivar that contained both carotenoids and anthocyanins, by cosuppression of CHS or DFR genes.