Metabolic Labeling and Digital Microfluidic Single‐Cell Sequencing for Single Bacterial Genotypic‐Phenotypic Analysis

• Published in: Small (Weinheim an der Bergstrasse, Germany) Vol. 20; no. 45; pp. e2402177 - n/a
• Main Authors: Guo, Junnan, Sun, Di, Li, Kunjie, Dai, Qi, Geng, Shichen, Yang, Yuanyuan, Mo, Mengwu, Zhu, Zhi, Shao, Chen, Wang, Wei, Song, Jia, Yang, Chaoyong, Zhang, Huimin
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.11.2024
• Subjects: Acid resistance; Amino acids; antibiotic resistance analysis; Antibiotics; Bacteria; digital microfluidics; Drug resistance; Fluorescence; Gene sequencing; Genomes; Labeling; metabolic labeling; Microfluidics; Resistance factors; single‐cell genome sequencing; digital microfluidics; metabolic labeling; antibiotic resistance analysis; single‐cell genome sequencing

Accurate assessment of phenotypic and genotypic characteristics of bacteria can facilitate comprehensive cataloguing of all the resistance factors for better understanding of antibiotic resistance. However, current methods primarily focus on individual phenotypic or genotypic profiles across differe...