NEMO–IKKβ Are Essential for IRF3 and NF-κB Activation in the cGAS–STING Pathway

• Published in: The Journal of immunology (1950) Vol. 199; no. 9; pp. 3222 - 3233
• Main Authors: Fang, Run, Wang, Chenguang, Jiang, Qifei, Lv, Mengze, Gao, Pengfei, Yu, Xiaoyu, Mu, Ping, Zhang, Rui, Bi, Sheng, Feng, Ji-Ming, Jiang, Zhengfan
• Format: Journal Article
• Language: English
• Published: United States American Association of Immunologists 01.11.2017
• Subjects: AMP; Animals; Cell culture; Cyclic GMP; Cytokines; HeLa Cells; Humans; I-kappa B Kinase - genetics; I-kappa B Kinase - immunology; Immune response; Interferon; Interferon regulatory factor 3; Interferon Regulatory Factor-3 - genetics; Interferon Regulatory Factor-3 - immunology; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - immunology; MCF-7 Cells; Membrane Proteins - genetics; Membrane Proteins - immunology; Mice; NF-kappa B - genetics; NF-kappa B - immunology; NF-κB protein; Nucleotidyltransferases - genetics; Nucleotidyltransferases - immunology; Signal Transduction - genetics; Signal Transduction - immunology; Ubiquitin

Cytosolic dsDNA activates the cyclic GMP-AMP synthase (cGAS)–stimulator of IFN genes (STING) pathway to produce cytokines, including type I IFNs. The roles of many critical proteins, including NEMO, IKKβ, and TBK1, in this pathway are unclear because of the lack of an appropriate system to study. In...