Eyelid skin and fibroadipose tissue MMP-1, MMP-3, TNF-α, and IL-6 levels in patients with inactive moderate-to-severe Graves' orbitopathy

• Published in: Orbit (Amsterdam) Vol. 44; no. 2; p. 190
• Main Authors: Yuksel, Nilay, Ozel-Turkcu, Ummuhani, Gok, Muslum, Saritas, Ozge, Yazici, Bulent
• Format: Journal Article
• Language: English
• Published: England 01.04.2025
• Subjects: Adipose Tissue - enzymology; Adipose Tissue - metabolism; Adult; Blepharoplasty; Enzyme-Linked Immunosorbent Assay; Eyelids - metabolism; Eyelids - surgery; Female; Graves Ophthalmopathy - metabolism; Graves Ophthalmopathy - surgery; Humans; Interleukin-6 - metabolism; Male; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase 3 - metabolism; Middle Aged; Prospective Studies; Skin - enzymology; Skin - metabolism; Tumor Necrosis Factor-alpha - metabolism; Extracellular matrix; fibrosis; tissue remodeling; matrix metalloproteinases; pro-inflammatory cytokines
• ISSN: 1744-5108
• DOI: 10.1080/01676830.2024.2414770

To evaluate matrix metalloprotease-1 (MMP-1), matrix metalloprotease-3 (MMP-3), tumor necrosis factor alpha (TNF-α), and interleukin-6 (IL-6) levels in the eyelid skin and fibroadipose tissue in patients with inactive moderate-to-severe Graves' orbitopathy (GO). This prospective study included 23 patients with inactive moderate-to-severe GO who underwent upper blepharoplasty and medial fat excision, and 22 age- and sex-matched healthy subjects. MMP-1, MMP-3, TNF-α, and IL-6 levels in the skin and fibroadipose tissue obtained during surgery were measured using the ELISA method. The mean MMP-1 level in the eyelid skin (  = .003) and the mean MMP-3 level in the fibroadipose tissue (  = .04) were significantly lower in the GO group compared to the healthy control group. There were no differences in other mediators in both tissues between the two groups (  > .05). The lower levels of proteolytic enzymes such as MMP-1 and MMP-3 in the eyelid skin and orbital fibroadipose tissue of patients with chronic inactive GO may play a role in the increase of collagen and glycosaminoglycans in orbital soft tissues.