Purification and characterization of Protein C activator from Agkistrodon acutus Venom

• Published in: Preparative biochemistry & biotechnology Vol. 50; no. 9; pp. 907 - 914
• Main Authors: Sun, Yao, Bao, Peng-Ju, Zhang, Gen-Bao
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 2020; Taylor & Francis Ltd
• Subjects: Agkistrodon; Agkistrodon - metabolism; Agkistrodon acutus venom; Animals; Anticoagulants; Assaying; Cardiovascular diseases; Coagulation; Electrophoresis; Electrophoresis, Polyacrylamide Gel; Enzymatic activity; Enzyme Activation - drug effects; Enzyme Activators - chemistry; Enzyme Activators - isolation & purification; Enzyme Activators - pharmacology; Gel chromatography; Gel electrophoresis; Ion exchange; Metalloproteinase; Polyacrylamide; Protein C; Protein C - agonists; Protein C - metabolism; Protein C activator; Protein purification; Proteins; Rabbits; Snake Venoms - chemistry; Snake Venoms - metabolism; Sodium dodecyl sulfate; Sodium lauryl sulfate; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thromboplastin; Venom; Zinc metalloproteinase; Agkistrodon acutus venom; Protein C; Protein C activator

Protein C (PC) plays an important role in the balance of coagulation and anticoagulation. Thus, the detection of PC activity is diagnostically significant for patients with cardiovascular diseases. Presently, the key methods to detect PC activity are the chromogenic assay and activated partial throm...