Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2102; p. 315
• Main Authors: Dobrovolsky, Vasily N, Cao, Xuefei, Bhalli, Javed A, Heflich, Robert H
• Format: Journal Article
• Language: English
• Published: United States 2020
• Subjects: Glycosyl phosphatidyl inositol; Flow cytometry; Gene mutation; CD59 surface marker; Antibodies; Reticulocytes; CD24 surface marker

The endogenous X-linked phosphatidyl inositol glycan class A gene (Pig-a) can be used as a reporter of in vivo somatic cell mutation in rats and mice. Pig-a mutant cells are deficient in specific protein surface markers and can be identified and quantified by immunofluorescent staining followed by h...