Expression and characterization of Edg-1 receptors in rat cardiomyocytes: calcium deregulation in response to sphingosine 1-phosphate

• Published in: European journal of biochemistry Vol. 267; no. 18; pp. 5679 - 5686
• Main Authors: Nakajima, N, Cavalli, A L, Biral, D, Glembotski, C C, McDonough, P M, Ho, P D, Betto, R, Sandoná, D, Palade, P T, Dettbarn, C A, Klepper, R E, Sabbadini, R A
• Format: Journal Article
• Language: English
• Published: England 01.09.2000
• Subjects: Animals; Blood Platelets - metabolism; Blotting, Western; Calcium - metabolism; Cardiac Pacing, Artificial; Cells, Cultured; Cloning, Molecular; DNA, Complementary - metabolism; Green Fluorescent Proteins; Immediate-Early Proteins - biosynthesis; Immediate-Early Proteins - genetics; Ligands; Luminescent Proteins - metabolism; Lysophospholipids; Myocardium - metabolism; Oligonucleotides, Antisense - genetics; Plasmids - metabolism; Rats; Receptors, Cell Surface; Receptors, G-Protein-Coupled; Receptors, Lysophospholipid; Reverse Transcriptase Polymerase Chain Reaction; Sarcoplasmic Reticulum - metabolism; Signal Transduction; Sphingosine - analogs & derivatives; Sphingosine - genetics; Sphingosine - metabolism; Sphingosine - pharmacology; Time Factors; Transfection

Recent evidence indicates that sphingolipids are produced by the heart during hypoxic stress and by blood platelets during thrombus formation. It is therefore possible that sphingolipids may influence heart cell function by interacting with G-protein-coupled receptors of the Edg family. In the prese...