IL-1β regulates the mouse Fas ligand expression in corneal endothelial cells

• Published in: Chinese science bulletin Vol. 52; no. 16; pp. 2210 - 2215
• Main Authors: Zhang, Jie, Yang, Ke, Tan, DeYong, Zeng, JunYing, Fine, Alan
• Format: Journal Article
• Language: English
• Published: Department of Biochemistry and Molecular Biology, School of Life Science, Yunnan University, Kunming 650091, China%Pulmonary Center and the Department of Biochemistry, Boston University School of Medicine, MA 02118, USA 01.08.2007
• Subjects: Fas配体; IL-1β; 动物实验; 基因表达; 角膜内皮细胞; promoter; Mouse; IL-1β; FasL; cornea
• ISSN: 1001-6538; 1861-9541
• DOI: 10.1007/s11434-007-0334-6

Constitutively expressed Fas ligand （FasL） in several distinct epithelial cell types appears to protect tissues by inducing apoptosis of Fas^＋ immune cells during inflammatory reactions. To study the relationship of FasL and inflammation process in cornea, we examined the effects of inflammatory cytokine IL-1β on the FasL production, expression and cytotoxic function in corneal endothelial cells. In this paper, we demonstrate that IL-1βinhibits the FasL production and expression in corneal endothelial cells. The promoter activities of FasL in these cells are reduced by IL-1β in a dose-dependent manner. Finally, we also find that IL-1β block the cytotoxic effects of FasL derived from corneal endothelial cells to the Fas^＋ target cells. These data support the view that FasL derived from corneal endothelial cells modulate inflammation within cornea.