An endogenous activator of renal glutamic acid decarboxylase effects of adenosine triphosphate, phosphate and chloride on the activity of this enzyme

• Published in: European journal of biochemistry Vol. 262; no. 3; pp. 696 - 703
• Main Authors: Turský, T, Bandzuchová, E
• Format: Journal Article
• Language: English
• Published: England 01.06.1999
• Subjects: Adenosine Triphosphate - pharmacology; Animals; Brain - enzymology; Cell-Free System; Enzyme Activation - drug effects; Glutamate Decarboxylase - isolation & purification; Glutamate Decarboxylase - metabolism; Kidney - enzymology; Phosphates - pharmacology; Rats; Rats, Wistar; Sodium Chloride - pharmacology; Solubility

The renal glutamic acid decarboxylase (GAD) differs from the brain and pancreatic enzyme by its strong binding to membranes that is not influenced by detergents. After centrifugation of freshly prepared homogenate of the rat renal cortex, only 10-15% of GAD activity was found in supernatants and 15-...