Genotyping microsatellite polymorphisms by agarose gel electrophoresis with ethidium bromide staining: application to quantitative trait loci analysis of seizure susceptibility in mice

• Published in: Psychiatric genetics Vol. 8; no. 4; p. 227
• Main Authors: Ferraro, T N, Schill, J F, Ballas, C, Mulholland, N, Golden, G T, Smith, G G, Buono, R J, Berrettini, W H
• Format: Journal Article
• Language: English
• Published: England 1998
• Subjects: Animals; Chromosome Mapping; Convulsants - toxicity; Crosses, Genetic; DNA - analysis; DNA - genetics; Drug Resistance; Electrophoresis, Agar Gel - methods; Ethidium; Female; Fluorescent Dyes; GABA-A Receptor Antagonists; Genetic Predisposition to Disease; Genotype; Male; Mice; Mice, Inbred C57BL - genetics; Mice, Inbred DBA - genetics; Microsatellite Repeats; Pentylenetetrazole - toxicity; Quantitative Trait, Heritable; Receptors, GABA-A - genetics; Seizures - chemically induced; Seizures - genetics; Staining and Labeling

Agarose gel electrophoresis with ethidium bromide staining (AGE/EBS) is an efficient and reliable method for analyzing microsatellite polymorphisms. We report the use of AGE/EBS for analyzing DNA microsatellite polymorphisms in a preliminary quantitative trait loci (QTL) study of seizure susceptibil...