Protein Expression Analysis by Western Blot and Protein-Protein Interactions

Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.Some Streptococcus pneumoniae proteins are important viru...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 1968; p. 101
Main Authors Cima-Cabal, María Dolores, Vazquez, Fernando, de Los Toyos, Juan R, Del Mar García-Suárez, María
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Pneumolysin
Urine
PpmA
Monoclonal antibody
Western-blot
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Abstract Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
AbstractList Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
Author Cima-Cabal, María Dolores
Del Mar García-Suárez, María
Vazquez, Fernando
de Los Toyos, Juan R
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  givenname: Juan R
  surname: de Los Toyos
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  givenname: María
  surname: Del Mar García-Suárez
  fullname: Del Mar García-Suárez, María
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  organization: Escuela Superior de Ingeniería y Tecnología (ESIT), Universidad Internacional de La Rioja (UNIR), Logroño, Spain. mar.garcia.suarez@unir.net
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Keywords Pneumolysin
Urine
PpmA
Monoclonal antibody
Western-blot
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Snippet Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is...
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Title Protein Expression Analysis by Western Blot and Protein-Protein Interactions
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