Protein Expression Analysis by Western Blot and Protein-Protein Interactions

Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.Some Streptococcus pneumoniae proteins are important viru...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1968; p. 101
Main Authors Cima-Cabal, María Dolores, Vazquez, Fernando, de Los Toyos, Juan R, Del Mar García-Suárez, María
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Pneumolysin
Urine
PpmA
Monoclonal antibody
Western-blot
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Summary:Western blot analysis is widely used for detecting protein expression, analysis of protein-protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9199-0_9