Distinct lactate metabolism between hepatocytes and myotubes revealed by live cell imaging with genetically encoded indicators

The process of glycolysis breaks down glycogen stored in muscles, producing lactate through pyruvate to generate energy. Excess lactate is then released into the bloodstream. When lactate reaches the liver, it is converted to glucose, which muscles utilize as a substrate to generate ATP. Although th...

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Published inBiochemical and biophysical research communications Vol. 694; p. 149416
Main Authors Horikoshi, Mina, Harada, Kazuki, Tsuno, Saki, Kitaguchi, Tetsuya, Hirai, Masami Yokota, Matsumoto, Mitsuharu, Terada, Shin, Tsuboi, Takashi
Format Journal Article
LanguageEnglish
Published United States 29.01.2024
Subjects
Adenosine Triphosphate - metabolism
Glucose - metabolism
Hepatocytes - metabolism
Lactic Acid
Muscle Fibers, Skeletal - metabolism
Pyruvates
Hepatocytes
Lactate metabolism
L6 cells
Cori cycle
Live cell imaging
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Abstract The process of glycolysis breaks down glycogen stored in muscles, producing lactate through pyruvate to generate energy. Excess lactate is then released into the bloodstream. When lactate reaches the liver, it is converted to glucose, which muscles utilize as a substrate to generate ATP. Although the biochemical study of lactate metabolism in hepatocytes and skeletal muscle cells has been extensive, the spatial and temporal dynamics of this metabolism in live cells are still unknown. We observed the dynamics of metabolism-related molecules in primary cultured hepatocytes and a skeletal muscle cell line upon lactate overload. Our observations revealed an increase in cytoplasmic pyruvate concentration in hepatocytes, which led to glucose release. Skeletal muscle cells exhibited elevated levels of lactate and pyruvate levels in both the cytoplasm and mitochondrial matrix. However, mitochondrial ATP levels remained unaffected, indicating that the increased lactate can be converted to pyruvate but is unlikely to be utilized for ATP production. The findings suggest that excess lactate in skeletal muscle cells is taken up into mitochondria with little contribution to ATP production. Meanwhile, lactate released into the bloodstream can be converted to glucose in hepatocytes for subsequent utilization in skeletal muscle cells.
AbstractList The process of glycolysis breaks down glycogen stored in muscles, producing lactate through pyruvate to generate energy. Excess lactate is then released into the bloodstream. When lactate reaches the liver, it is converted to glucose, which muscles utilize as a substrate to generate ATP. Although the biochemical study of lactate metabolism in hepatocytes and skeletal muscle cells has been extensive, the spatial and temporal dynamics of this metabolism in live cells are still unknown. We observed the dynamics of metabolism-related molecules in primary cultured hepatocytes and a skeletal muscle cell line upon lactate overload. Our observations revealed an increase in cytoplasmic pyruvate concentration in hepatocytes, which led to glucose release. Skeletal muscle cells exhibited elevated levels of lactate and pyruvate levels in both the cytoplasm and mitochondrial matrix. However, mitochondrial ATP levels remained unaffected, indicating that the increased lactate can be converted to pyruvate but is unlikely to be utilized for ATP production. The findings suggest that excess lactate in skeletal muscle cells is taken up into mitochondria with little contribution to ATP production. Meanwhile, lactate released into the bloodstream can be converted to glucose in hepatocytes for subsequent utilization in skeletal muscle cells.The process of glycolysis breaks down glycogen stored in muscles, producing lactate through pyruvate to generate energy. Excess lactate is then released into the bloodstream. When lactate reaches the liver, it is converted to glucose, which muscles utilize as a substrate to generate ATP. Although the biochemical study of lactate metabolism in hepatocytes and skeletal muscle cells has been extensive, the spatial and temporal dynamics of this metabolism in live cells are still unknown. We observed the dynamics of metabolism-related molecules in primary cultured hepatocytes and a skeletal muscle cell line upon lactate overload. Our observations revealed an increase in cytoplasmic pyruvate concentration in hepatocytes, which led to glucose release. Skeletal muscle cells exhibited elevated levels of lactate and pyruvate levels in both the cytoplasm and mitochondrial matrix. However, mitochondrial ATP levels remained unaffected, indicating that the increased lactate can be converted to pyruvate but is unlikely to be utilized for ATP production. The findings suggest that excess lactate in skeletal muscle cells is taken up into mitochondria with little contribution to ATP production. Meanwhile, lactate released into the bloodstream can be converted to glucose in hepatocytes for subsequent utilization in skeletal muscle cells.
The process of glycolysis breaks down glycogen stored in muscles, producing lactate through pyruvate to generate energy. Excess lactate is then released into the bloodstream. When lactate reaches the liver, it is converted to glucose, which muscles utilize as a substrate to generate ATP. Although the biochemical study of lactate metabolism in hepatocytes and skeletal muscle cells has been extensive, the spatial and temporal dynamics of this metabolism in live cells are still unknown. We observed the dynamics of metabolism-related molecules in primary cultured hepatocytes and a skeletal muscle cell line upon lactate overload. Our observations revealed an increase in cytoplasmic pyruvate concentration in hepatocytes, which led to glucose release. Skeletal muscle cells exhibited elevated levels of lactate and pyruvate levels in both the cytoplasm and mitochondrial matrix. However, mitochondrial ATP levels remained unaffected, indicating that the increased lactate can be converted to pyruvate but is unlikely to be utilized for ATP production. The findings suggest that excess lactate in skeletal muscle cells is taken up into mitochondria with little contribution to ATP production. Meanwhile, lactate released into the bloodstream can be converted to glucose in hepatocytes for subsequent utilization in skeletal muscle cells.
ArticleNumber 149416
Author Horikoshi, Mina
Terada, Shin
Tsuno, Saki
Harada, Kazuki
Hirai, Masami Yokota
Matsumoto, Mitsuharu
Kitaguchi, Tetsuya
Tsuboi, Takashi
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  givenname: Kazuki
  surname: Harada
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  givenname: Saki
  surname: Tsuno
  fullname: Tsuno, Saki
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  givenname: Takashi
  surname: Tsuboi
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  email: takatsuboi@bio.c.u-tokyo.ac.jp
  organization: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8654, Japan; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902, Japan. Electronic address: takatsuboi@bio.c.u-tokyo.ac.jp
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Cites_doi 10.1152/ajpendo.00594.2005
10.1016/S0006-3495(00)76468-X
10.1016/j.crphys.2020.07.002
10.2330/joralbiosci1965.12.293
10.1038/s41598-020-76440-4
10.1002/anie.201804304
10.1007/s00421-017-3795-6
10.1016/j.redox.2019.101339
10.1083/jcb.51.3.621
10.1016/j.xpro.2020.100086
10.1113/JP278930
10.1016/j.chembiol.2021.06.002
10.1016/0003-9861(86)90323-1
10.1016/0003-9861(87)90507-8
10.1073/pnas.96.3.1129
10.3389/fnins.2015.00022
10.1152/jappl.1999.87.5.1713
10.1007/s00421-006-0334-2
10.1152/jappl.1988.65.2.509
10.1016/S0021-9258(18)84849-9
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Keywords Hepatocytes
Lactate metabolism
L6 cells
Cori cycle
Live cell imaging
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References Katz (10.1016/j.bbrc.2023.149416_bib21) 1988; 65
Kline (10.1016/j.bbrc.2023.149416_bib3) 1986; 246
Mita (10.1016/j.bbrc.2023.149416_bib17) 2022; 29
Benelli (10.1016/j.bbrc.2023.149416_bib19) 2007; 99
Hashimoto (10.1016/j.bbrc.2023.149416_bib6) 2006; 290
Brooks (10.1016/j.bbrc.2023.149416_bib10) 1999; 87
Glancy (10.1016/j.bbrc.2023.149416_bib9) 2021; 599
Young (10.1016/j.bbrc.2023.149416_bib11) 2020; 28
Charni-Natan (10.1016/j.bbrc.2023.149416_bib13) 2020; 1
Brooks (10.1016/j.bbrc.2023.149416_bib5) 1999; 96
Ferguson (10.1016/j.bbrc.2023.149416_bib1) 2018; 118
Sankaranarayanan (10.1016/j.bbrc.2023.149416_bib14) 2000; 79
Takahashi (10.1016/j.bbrc.2023.149416_bib22) 2020; 3
Rogatzki (10.1016/j.bbrc.2023.149416_bib7) 2015; 9
Martín (10.1016/j.bbrc.2023.149416_bib15) 2013; 8
Brandt (10.1016/j.bbrc.2023.149416_bib4) 1987; 259
Cori (10.1016/j.bbrc.2023.149416_bib2) 1925; 65
Baba (10.1016/j.bbrc.2023.149416_bib8) 1971; 51
Tsuno (10.1016/j.bbrc.2023.149416_bib12) 2023
Harada (10.1016/j.bbrc.2023.149416_bib16) 2020; 10
Arai (10.1016/j.bbrc.2023.149416_bib18) 2018; 57
Suzuki (10.1016/j.bbrc.2023.149416_bib20) 1970; 12
References_xml – volume: 290
  start-page: E1237
  year: 2006
  ident: 10.1016/j.bbrc.2023.149416_bib6
  article-title: Colocalization of MCT1, CD147, and LDH in mitochondrial inner membrane of L6 muscle cells: evidence of a mitochondrial lactate oxidation complex
  publication-title: Am. J. Physiol. Endocrinol. Metab.
  doi: 10.1152/ajpendo.00594.2005
  contributor:
    fullname: Hashimoto
– volume: 79
  start-page: 2199
  year: 2000
  ident: 10.1016/j.bbrc.2023.149416_bib14
  article-title: The use of pHluorins for optical measurements of presynaptic activity
  publication-title: Biophys. J.
  doi: 10.1016/S0006-3495(00)76468-X
  contributor:
    fullname: Sankaranarayanan
– volume: 3
  start-page: 34
  year: 2020
  ident: 10.1016/j.bbrc.2023.149416_bib22
  article-title: Effect of post-exercise lactate administration on glycogen repletion and signaling activation in different types of mouse skeletal muscle
  publication-title: Curr. Res. Physiol.
  doi: 10.1016/j.crphys.2020.07.002
  contributor:
    fullname: Takahashi
– volume: 12
  start-page: 293
  year: 1970
  ident: 10.1016/j.bbrc.2023.149416_bib20
  article-title: Lactic dehydrogenase isoenzyme
  publication-title: Jap. J. Oral Biol.
  doi: 10.2330/joralbiosci1965.12.293
  contributor:
    fullname: Suzuki
– volume: 10
  year: 2020
  ident: 10.1016/j.bbrc.2023.149416_bib16
  article-title: Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-020-76440-4
  contributor:
    fullname: Harada
– volume: 57
  start-page: 10873
  year: 2018
  ident: 10.1016/j.bbrc.2023.149416_bib18
  article-title: RGB-color intensiometric indicators visualize spatiotemporal dynamics of ATP in single cells
  publication-title: Angew. Chem., Int. Ed. Engl.
  doi: 10.1002/anie.201804304
  contributor:
    fullname: Arai
– volume: 118
  start-page: 691
  year: 2018
  ident: 10.1016/j.bbrc.2023.149416_bib1
  article-title: Lactate metabolism: historical context, prior misinterpretations, and current understanding
  publication-title: Eur. J. Appl. Physiol.
  doi: 10.1007/s00421-017-3795-6
  contributor:
    fullname: Ferguson
– volume: 28
  year: 2020
  ident: 10.1016/j.bbrc.2023.149416_bib11
  article-title: Lactate dehydrogenase supports lactate oxidation in mitochondria isolated from different mouse tissue
  publication-title: Redox Biol.
  doi: 10.1016/j.redox.2019.101339
  contributor:
    fullname: Young
– year: 2023
  ident: 10.1016/j.bbrc.2023.149416_bib12
  article-title: Mitochondrial ATP concentration decreases immediately after glucose administration to glucose-deprived hepatocytes
  publication-title: FEBS Open Bio
  contributor:
    fullname: Tsuno
– volume: 51
  start-page: 621
  year: 1971
  ident: 10.1016/j.bbrc.2023.149416_bib8
  article-title: Histochemistry of lactic dehydrogenase in heart and pectoralis muscles of rat
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.51.3.621
  contributor:
    fullname: Baba
– volume: 1
  year: 2020
  ident: 10.1016/j.bbrc.2023.149416_bib13
  article-title: Protocol for primary mouse hepatocyte isolation
  publication-title: STAR Protoc
  doi: 10.1016/j.xpro.2020.100086
  contributor:
    fullname: Charni-Natan
– volume: 599
  start-page: 863
  year: 2021
  ident: 10.1016/j.bbrc.2023.149416_bib9
  article-title: Mitochondrial lactate metabolism: history and implications for exercise and disease
  publication-title: J. Physiol.
  doi: 10.1113/JP278930
  contributor:
    fullname: Glancy
– volume: 8
  year: 2013
  ident: 10.1016/j.bbrc.2023.149416_bib15
  article-title: A genetically encoded FRET lactate sensor and its use to detect the Warburg effect in single cancer cells
  publication-title: PLoS One
  contributor:
    fullname: Martín
– volume: 29
  start-page: 98
  year: 2022
  ident: 10.1016/j.bbrc.2023.149416_bib17
  article-title: Development of red genetically encoded biosensor for visualization of intracellular glucose dynamics
  publication-title: Cell Chem. Biol.
  doi: 10.1016/j.chembiol.2021.06.002
  contributor:
    fullname: Mita
– volume: 246
  start-page: 673
  year: 1986
  ident: 10.1016/j.bbrc.2023.149416_bib3
  article-title: Localization of L-lactate dehydrogenase in mitochondria
  publication-title: Arch. Biochem. Biophys.
  doi: 10.1016/0003-9861(86)90323-1
  contributor:
    fullname: Kline
– volume: 259
  start-page: 412
  year: 1987
  ident: 10.1016/j.bbrc.2023.149416_bib4
  article-title: Lactate dehydrogenase in rat mitochondria
  publication-title: Arch. Biochem. Biophys.
  doi: 10.1016/0003-9861(87)90507-8
  contributor:
    fullname: Brandt
– volume: 96
  start-page: 1129
  year: 1999
  ident: 10.1016/j.bbrc.2023.149416_bib5
  article-title: Role of mitochondrial lactate dehydrogenase and lactate oxidation in the ‘intra-cellular lactate shuttle’
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.96.3.1129
  contributor:
    fullname: Brooks
– volume: 9
  start-page: 22
  year: 2015
  ident: 10.1016/j.bbrc.2023.149416_bib7
  article-title: Lactate is always the end product of glycolysis
  publication-title: Front. Neurosci.
  doi: 10.3389/fnins.2015.00022
  contributor:
    fullname: Rogatzki
– volume: 87
  start-page: 1713
  year: 1999
  ident: 10.1016/j.bbrc.2023.149416_bib10
  article-title: MCT1 in cardiac and skeletal muscle mitochondria
  publication-title: J. Appl. Physiol.
  doi: 10.1152/jappl.1999.87.5.1713
  contributor:
    fullname: Brooks
– volume: 99
  start-page: 685
  year: 2007
  ident: 10.1016/j.bbrc.2023.149416_bib19
  article-title: Assessment of post-competition peak blood lactate in male and female master swimmers aged 40–79 years and its relationship with swimming performance
  publication-title: Eur. J. Appl. Physiol.
  doi: 10.1007/s00421-006-0334-2
  contributor:
    fullname: Benelli
– volume: 65
  start-page: 509
  year: 1988
  ident: 10.1016/j.bbrc.2023.149416_bib21
  article-title: Regulation of lactic acid production during exercise
  publication-title: J. Appl. Physiol.
  doi: 10.1152/jappl.1988.65.2.509
  contributor:
    fullname: Katz
– volume: 65
  start-page: 397
  year: 1925
  ident: 10.1016/j.bbrc.2023.149416_bib2
  article-title: The carbohydrate metabolism of tumors II. Changes in the sugar, lactic acid, and CO2- combining Power of Blood Passing through a tumor
  publication-title: J. Biol. Chem.
  doi: 10.1016/S0021-9258(18)84849-9
  contributor:
    fullname: Cori
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Snippet The process of glycolysis breaks down glycogen stored in muscles, producing lactate through pyruvate to generate energy. Excess lactate is then released into...
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StartPage 149416
SubjectTerms Adenosine Triphosphate - metabolism
Glucose - metabolism
Hepatocytes - metabolism
Lactic Acid
Muscle Fibers, Skeletal - metabolism
Pyruvates
Title Distinct lactate metabolism between hepatocytes and myotubes revealed by live cell imaging with genetically encoded indicators
URI https://www.ncbi.nlm.nih.gov/pubmed/38147697
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