Survey of Viruses Infecting Basella alba in Hawaii
Malabar spinach plants ( , Basellaceae) with leaves exhibiting symptoms of mosaic, rugosity, and malformation were found in a community garden on Oahu, HI in 2018. Preliminary studies using enzyme-linked immunosorbent assay and reverse-transcription (RT)-PCR identified Basella rugose mosaic virus (B...
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Published in | Plant disease Vol. 107; no. 4; p. 1022 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.04.2023
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Subjects | |
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Abstract | Malabar spinach plants (
, Basellaceae) with leaves exhibiting symptoms of mosaic, rugosity, and malformation were found in a community garden on Oahu, HI in 2018. Preliminary studies using enzyme-linked immunosorbent assay and reverse-transcription (RT)-PCR identified Basella rugose mosaic virus (BaRMV) in symptomatic plants. However, nucleotide sequence analysis of RT-PCR amplicons indicated that additional potyviruses were also present in the symptomatic Malabar spinach. High-throughput sequencing (HTS) analysis was conducted on ribosomal RNA-depleted composite RNA samples of potyvirus-positive plants from three locations. Assembled contigs shared sequences similar to BaRMV, chilli veinal mottle virus (ChiVMV), Alternanthera mosaic virus (AltMV), Basella alba endornavirus (BaEV), broad bean wilt virus 2 (BBWV2), and Iresine viroid 1. Virus- and viroid-specific primers were designed based on HTS sequencing results and used in RT-PCR and Sanger sequencing to confirm the presence of these viruses and the viroid. We tested 63 additional samples from six community gardens for a survey of viruses in Malabar spinach and found that 21 of them were positive for BaRMV, 57 for ChiVMV, 21 for AltMV, 19 for BaEV, and 14 for BBWV2. This is the first characterization of the virome from
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AbstractList | Malabar spinach plants (
, Basellaceae) with leaves exhibiting symptoms of mosaic, rugosity, and malformation were found in a community garden on Oahu, HI in 2018. Preliminary studies using enzyme-linked immunosorbent assay and reverse-transcription (RT)-PCR identified Basella rugose mosaic virus (BaRMV) in symptomatic plants. However, nucleotide sequence analysis of RT-PCR amplicons indicated that additional potyviruses were also present in the symptomatic Malabar spinach. High-throughput sequencing (HTS) analysis was conducted on ribosomal RNA-depleted composite RNA samples of potyvirus-positive plants from three locations. Assembled contigs shared sequences similar to BaRMV, chilli veinal mottle virus (ChiVMV), Alternanthera mosaic virus (AltMV), Basella alba endornavirus (BaEV), broad bean wilt virus 2 (BBWV2), and Iresine viroid 1. Virus- and viroid-specific primers were designed based on HTS sequencing results and used in RT-PCR and Sanger sequencing to confirm the presence of these viruses and the viroid. We tested 63 additional samples from six community gardens for a survey of viruses in Malabar spinach and found that 21 of them were positive for BaRMV, 57 for ChiVMV, 21 for AltMV, 19 for BaEV, and 14 for BBWV2. This is the first characterization of the virome from
. |
Author | Suzuki, Jon Y Al Rwahnih, Maher Larrea-Sarmiento, Adriana Borth, Wayne Hu, John Olmedo-Velarde, Alejandro Melzer, Michael Wall, Marisa M Wang, Xupeng |
Author_xml | – sequence: 1 givenname: Xupeng orcidid: 0000-0001-8520-1714 surname: Wang fullname: Wang, Xupeng organization: Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822 – sequence: 2 givenname: Adriana orcidid: 0000-0003-3355-1522 surname: Larrea-Sarmiento fullname: Larrea-Sarmiento, Adriana organization: Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822 – sequence: 3 givenname: Alejandro orcidid: 0000-0003-1688-0455 surname: Olmedo-Velarde fullname: Olmedo-Velarde, Alejandro organization: Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822 – sequence: 4 givenname: Maher orcidid: 0000-0003-1589-9234 surname: Al Rwahnih fullname: Al Rwahnih, Maher organization: Department of Plant Pathology, University of California, Davis, CA 95616 – sequence: 5 givenname: Wayne surname: Borth fullname: Borth, Wayne organization: Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822 – sequence: 6 givenname: Jon Y surname: Suzuki fullname: Suzuki, Jon Y organization: United States Department of Agriculture, Agricultural Research Service, Pacific Basin Agricultural Research Center, Hilo, HI 96720 – sequence: 7 givenname: Marisa M surname: Wall fullname: Wall, Marisa M organization: United States Department of Agriculture, Agricultural Research Service, Pacific Basin Agricultural Research Center, Hilo, HI 96720 – sequence: 8 givenname: Michael orcidid: 0000-0003-0390-3857 surname: Melzer fullname: Melzer, Michael organization: Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822 – sequence: 9 givenname: John orcidid: 0000-0003-3003-4140 surname: Hu fullname: Hu, John organization: Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822 |
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Snippet | Malabar spinach plants (
, Basellaceae) with leaves exhibiting symptoms of mosaic, rugosity, and malformation were found in a community garden on Oahu, HI in... |
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Title | Survey of Viruses Infecting Basella alba in Hawaii |
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