Production, Purification, and Characterization of ¹⁵N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements

• Published in: Methods in enzymology Vol. 566; p. 305
• Main Authors: Reddy, Prasad T, Jaruga, Pawel, Nelson, Bryant C, Lowenthal, Mark S, Jemth, Ann-Sofie, Loseva, Olga, Coskun, Erdem, Helleday, Thomas, Dizdaroglu, Miral
• Format: Journal Article
• Language: English
• Published: United States 2016
• Subjects: DNA Damage - genetics; DNA Repair - genetics; Humans; Mass Spectrometry - methods; Nitrogen Isotopes - chemistry; Proteins - chemistry; Proteins - genetics; Proteins - isolation & purification; Staining and Labeling - methods; N-labeled proteins; Protein measurements; DNA repair proteins; DNA repair; Mass spectrometry; DNA damage

Oxidatively induced DNA damage is caused in living organisms by a variety of damaging agents, resulting in the formation of a multiplicity of lesions, which are mutagenic and cytotoxic. Unless repaired by DNA repair mechanisms before DNA replication, DNA lesions can lead to genomic instability, whic...