High abundance of butyrate-producing bacteria in the naso-oropharynx of SARS-CoV-2-infected persons in an African population: implications for low disease severity

• Published in: BMC infectious diseases Vol. 24; no. 1; pp. 1020 - 12
• Main Authors: Akorli, Jewelna, Opoku, Millicent, Appiah-Twum, Francis, Akpo, Margaret Sena, Ismail, Rahmat Yusif, Boamah, Georgina Yaa Kwartemaa, Obeng-Aboagye, Elizabeth, Adu-Asamoah, Dina, Donkor, Irene Owusu
• Format: Journal Article
• Language: English
• Published: BioMed Central Ltd 20.09.2024; BMC
• Subjects: Bacillota; Bacteria; Bacteroidota; Butyrate producers; COVID-19; Health aspects; Medical research; Medicine, Experimental; Nasal fossa; Naso-oropharyngeal microbiome; Oropharynx; Physiological aspects; SARS-CoV-2; Africa
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-024-09948-z

Abstract Background The association of the oral microbiome with SARS-CoV-2 infections and disease progression has been documented in European, Asian, and American populations but not in Africa. Methods We conducted a study in Ghana to evaluate and compare the naso-oropharyngeal microbiome in SARS-CoV-2-infected and uninfected persons before (pre-vaccine) and after vaccine availability (post-vaccine) in the country. 16S rRNA V3-V4 variable region was sequenced and analysed from DNA extracted from naso-oropharyngeal swabs. Results Considering only the infection status, infected and uninfected groups had no difference in their within-group diversity and was evident in the study population pre- and post-vaccine availability. The introduction of vaccines reduced the diversity of the naso-oropharyngeal microbiome particularly among SARS-CoV-2 positive persons and, vaccinated individuals (both infected and uninfected) had higher microbial diversity compared to their unvaccinated counterparts. SARS-CoV-2-positive and -negative individuals were largely compositionally similar varying by 4–7% but considering vaccination*infection statuses, the genetic distance increased to 12% ( P = 0.003) and was mainly influenced by vaccination. Common among the pre- and post-vaccine samples, Atopobium and Finegoldia were abundant in infected and uninfected individuals, respectively. Bacteria belonging to major butyrate-producing phyla, Bacillota (particularly class Clostridia ) and Bacteroidota showed increased abundance more strikingly in infected individuals before vaccines were available. They reduced significantly after vaccines were introduced into the country with Fusobacterium and Lachnoanaerobaculum being the only common bacteria between pre-vaccine infected persons and vaccinated individuals, suggesting that natural infection and vaccination correlate with high abundance of short-chain fatty acids. Conclusion Our results show, in an African cohort, the abundance of bacteria taxa known for their protective pathophysiological processes, especially during infection, suggesting that this population is protected against severe COVID-19. The immune-related roles of the members of Bacillota and Bacteroidota that were found associated with infection and vaccination require further studies, and how these may be linked to ethnicity, diet and age. We also recommend expansion of microbiome–disease association studies across Africa to identify possible bacterial-mediated therapeutics for emerging infections.