Reconstitution of the mitochondrial ATP-dependent potassium channel into bilayer lipid membrane

• Published in: Journal of bioenergetics and biomembranes Vol. 31; no. 2; pp. 159 - 163
• Main Authors: Mironova, G D, Skarga, Y Y, Grigoriev, S M, Negoda, A E, Kolomytkin, O V, Marinov, B S
• Format: Journal Article
• Language: English
• Published: United States 01.04.1999
• Subjects: Adenosine Triphosphate - pharmacology; Adenosine Triphosphate - physiology; Animals; Intracellular Membranes - physiology; Lipid Bilayers; Male; Membrane Potentials - drug effects; Membrane Potentials - physiology; Mitochondria, Liver - physiology; Potassium - physiology; Potassium Channels - isolation & purification; Potassium Channels - physiology; Potassium Chloride - pharmacology; Rats; Rats, Wistar; Seasons; Substrate Specificity

Electrical properties and regulation of the mitochondrial ATP-dependent potassium channel were studied. The channel protein was solubilized from the mitochondrial membrane using an ethanol/water mixture. Reconstituted into a bilayer lipid membrane BLM), the protein formed a slightly voltage-dependen...