Evaluation of the effectiveness of potato improvement in in vitro culture on optimized nutrient media

• Published in: BIO Web of Conferences Vol. 36; p. 5007
• Main Authors: Gneusheva, I.A., Gavrilova, A.Yu, Gagarina, I.N., Gorkova, I.V., Solokhina, I.Yu
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Les Ulis EDP Sciences 01.01.2021
• Subjects: Antiinfectives and antibacterials; Antiviral activity; Culture media; Meristems; Nutrients; Potatoes; Seeds; Survival; Vegetables; Viruses
• DOI: 10.1051/bioconf/20213605007
• ISSN: 2117-4458; 2273-1709

Studies have been conducted on the use of an optimized Murashige-Scoog nutrient medium to obtain healthy seeds of promising potato varieties. It was found that the use of agarized modified Murashige-Scoog nutrient medium with the addition of exometabolites of fungi of the genus Trichoderma lixii 1.5 mg/l with antimicrobial properties and the antiviral drug “Cycloferon” in a concentration of 0.01% contributes to an increase in the number of virus-free potato regenerants when the method of apical meristems and in vitro improvement is used together. The effectiveness of using the modified medium is confirmed by the following criteria: active growth of test tube potato micro-plants, which is expressed in optimal morphometric indicators on the example of the potato variety “Rosara”; the size of the isolated meristems is 10 mm, which contributes to the output of healthy plants; the survival rate of regenerating plants. It is shown that when using the modified medium, an average of 35-50% of regenerating plants were obtained, depending on the potato variety, free from M, S viruses.