Bioreaction Network Topology and Metabolic Flux Ratio Analysis by Biosynthetic Fractional 13C Labeling and Two-Dimensional NMR Spectroscopy

• Published in: Metabolic engineering Vol. 1; no. 3; pp. 189 - 197
• Main Authors: Szyperski, Thomas, Glaser, Ralf W, Hochuli, Michel, Fiaux, Jocelyne, Sauer, Uwe, Bailey, James E, Wüthrich, Kurt
• Format: Journal Article
• Language: English
• Published: Belgium Elsevier Inc 01.07.1999
• Subjects: 2D NMR; amino acid biosynthesis; Amino Acids - biosynthesis; Amino Acids - isolation & purification; Biomass; Biomedical Engineering; Bioreactors; biosynthetic fractional 13C labeling; Biotechnology; Carbon Isotopes; central carbon metabolism; Haloarcula - metabolism; in vivo flux ratios; Magnetic Resonance Spectroscopy - methods; metabolic flux balancing; METAFoR analysis; Software; central carbon metabolism; metabolic flux balancing; 2D NMR; in vivo flux ratios; METAFoR analysis; biosynthetic fractional 13C labeling; amino acid biosynthesis

Biosynthetically directed fractional 13C labeling of the proteinogenic amino acids is achieved by feeding a mixture of uniformly 13C-labeled and unlabeled carbon source compounds into a bioreaction network. Analysis of the resulting labeling pattern enables both a comprehensive characterization of t...