Biosynthesis of l -theanine via one-step purification and immobilization enzyme system

• Published in: Journal of applied microbiology Vol. 136; no. 3
• Main Authors: Fan, Chao, Qi, Jiakun, Zhang, Chunzhi
• Format: Journal Article
• Language: English
• Published: England 03.03.2025
• Subjects: Amide Synthases - genetics; Amide Synthases - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biocatalysis; Enzymes, Immobilized - genetics; Enzymes, Immobilized - metabolism; Escherichia coli - genetics; Glutamates - biosynthesis; Listeria monocytogenes - enzymology; Listeria monocytogenes - genetics; Phosphotransferases (Phosphate Group Acceptor) - genetics; Phosphotransferases (Phosphate Group Acceptor) - metabolism; anchoring tags; GEM particles; one-step purification and immobilization (OSPI); l-theanine production

l-theanine, a non-protein amino acid derived from green tea, was synthesized by a relatively substantial amount of γ-glutamylmethylamide synthetase (GMAS) and polyphosphate kinase (PPK) without efficient recycling. This study establishes a cost-efficient, industrially scalable, and continuous biocat...