The design of a new mutation model for active genes: expression of the Escherichia coli lac operon in mammalian cells

• Published in: Mutation research Vol. 382; no. 1-2; pp. 21 - 33
• Main Authors: Van Sloun, Petra P.H, Lohman, Paul H.M, Vrieling, Harry
• Format: Journal Article
• Language: English
• Published: Netherlands 01.09.1997
• Subjects: Animals; Bacterial Proteins - genetics; beta-Galactosidase - genetics; beta-Galactosidase - metabolism; Escherichia coli - genetics; Escherichia coli Proteins; Gene Expression Regulation; Genes, Reporter - genetics; Genetic Vectors - genetics; L Cells (Cell Line); Lac Operon - genetics; Lac Repressors; Mice; Mice, Transgenic - genetics; Models, Genetic; Mutation; Repressor Proteins - genetics; Transgenes - genetics

The design of a novel transgenic mouse model is described that should allow analysis of mutations at a single cell level in all tissues of a model animal. The model is based on the correct regulation of the Escherichia coli lac operon in mammalian cells. Induction of a mutation in the lacI gene will...