Immunization with hybrid recombinant Mycobacterium tuberculosis H37Rv proteins increases the TH1 cytokine response in mice following a pulmonary instillation of irradiated mycobacteria

• Published in: Vaccine Vol. 26; no. 34; pp. 4396 - 4402
• Main Authors: WALTON, Chad B, INOS, Ana Blossom H, ANDRES, Osier A, JUBE, Sandro, DE COUET, Heinz Gert, DOUGLAS, James T, PATEK, Paul Q, BORTHAKUR, Dulal
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier 12.08.2008
• Subjects: Animals; Applied microbiology; Bacterial diseases; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; CD8-Positive T-Lymphocytes - immunology; Cytokines - biosynthesis; Epitopes, T-Lymphocyte - genetics; Fundamental and applied biological sciences. Psychology; Human bacterial diseases; Infectious diseases; Lung - immunology; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Microbiology; Miscellaneous; Mycobacterium bovis - genetics; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - immunology; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Th1 Cells - immunology; Tuberculosis and atypical mycobacterial infections; Tuberculosis Vaccines - genetics; Tuberculosis Vaccines - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Dendritic cell; Immunization; Rodentia; Cytokine; Th1 lymphocyte; Helper cell; Vaccine; Mycobacterial infection; Subunit; Tuberculosis,Subunit immunogens,Cross-presentation,Dendritic cell,Macropinocytosis,IFN-γ; Infection; Vertebrata; Mammalia; Tuberculosis; Mycobacterium tuberculosis; Mouse; Mycobacteriales; T-Lymphocyte; Bacteriosis; Mycobacteriaceae; Bacteria; Actinomycetes; Recombinant protein; Gamma interferon

The aim of this research was to identify subunit immunogens that can generate enhanced CD8 T cell and TH1 responses against Mycobacterium tuberculosis. A genomic comparison of the M. tuberculosis H(37)R(V) and M. bovis BCG identified 61 proteins that are unique to H(37)R(V). Further screening of the...