A simple, sensitive and label-free method for miRNA analysis in gastric cancer via catalytic hairpin assembly assisted programming of split-G-quadruplexes

Accurate analysis of miRNA is valuable for the diagnosis of various diseases. Herein, a sensitive and accurate fluorescence method was developed for miRNA detection based on catalytic hairpin assembly (CHA) and split-G-quadruplex (split-G4) based signal reactions. The presence of target miRNA activa...

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Bibliographic Details
Published inAnalytical methods Vol. 15; no. 34; pp. 4236 - 4242
Main Authors Ma, Xiaoli, Liu, Hongmei, Tao, Siyu
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 31.08.2023
Subjects
Assembly
Diagnosis
Fluorescent dyes
Fluorescent indicators
Gastric cancer
Labels
miRNA
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ISSN1759-9660
1759-9679
1759-9679
DOI10.1039/D3AY00989K

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Summary:Accurate analysis of miRNA is valuable for the diagnosis of various diseases. Herein, a sensitive and accurate fluorescence method was developed for miRNA detection based on catalytic hairpin assembly (CHA) and split-G-quadruplex (split-G4) based signal reactions. The presence of target miRNA activated the CHA process through unfolding the H1 probe, which could continuously induce the proximity of split-G4. The formed intact G4 can be specifically recognized by the commercial fluorescent dye ThT (thioflavin T), allowing for the highly sensitive, label-free detection of miRNAs. By utilizing split-G4 to generate a signal, the method exhibited a low background signal and a high reliability. In addition, the method is demonstrated to be applied for clinical sample detection, implying its promising prospect for disease diagnosis.
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ISSN:1759-9660
1759-9679
1759-9679
DOI:10.1039/D3AY00989K