Assessment of three human FcεRI-transfected RBL cell-lines for identifying IgE induced degranulation utilizing peanut-allergic patient sera and peanut protein extract

• Published in: Regulatory toxicology and pharmacology Vol. 51; no. 3; pp. 288 - 294
• Main Authors: Ladics, G.S., van Bilsen, J.H.M., Brouwer, H.M.H., Vogel, L., Vieths, S., Knippels, L.M.J.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.08.2008
• Subjects: Arachis hypogaea; Biological activity; Human FcεRI; Humanized rat basophilic leukemia cells; Mediator release; Peanut-allergic subjects; Protein cross-reactivity; Peanut-allergic subjects; Humanized rat basophilic leukemia cells; Human FcεRI; Mediator release; Protein cross-reactivity; Biological activity

Specific IgE sera screening studies are employed to investigate protein cross-reactivity. Such nonfunctional immunochemical methods cannot measure the biological activity of proteins. Therefore, an assay using RBL cells transfected with human FcεRI was developed. Our objective was to evaluate the de...