Exploring Mitochondrial Interactions with Pulsed Electromagnetic Fields: An Insightful Inquiry into Strategies for Addressing Neuroinflammation and Oxidative Stress in Diabetic Neuropathy

• Published in: International journal of molecular sciences Vol. 25; no. 14; p. 7783
• Main Authors: Chianese, Diego, Bonora, Massimo, Sambataro, Maria, Sambato, Luisa, Paola, Luca Dalla, Tremoli, Elena, Cappucci, Ilenia Pia, Scatto, Marco, Pinton, Paolo, Picari, Massimo, Ferroni, Letizia, Zavan, Barbara
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 16.07.2024
• Subjects: Antioxidants; Apoptosis; Biosynthesis; Cell cycle; Chemokines; complex magnetic fields; Cytokines; Diabetes; diabetic foot; Diabetic neuropathy; Electromagnetism; Homeostasis; Hyperglycemia; Hypoxia; Inflammation; Kinases; Magnetic fields; MicroRNAs; Nervous system; Nitric oxide; Oxidative stress; Proteins; pulsed electromagnetic fields; reactive oxygen species; Tumor necrosis factor-TNF; wound healing
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25147783

Pulsed electromagnetic fields (PEMFs) are recognized for their potential in regenerative medicine, offering a non-invasive avenue for tissue rejuvenation. While prior research has mainly focused on their effects on bone and dermo-epidermal tissues, the impact of PEMFs on nervous tissue, particularly in the context of neuropathy associated with the diabetic foot, remains relatively unexplored. Addressing this gap, our preliminary in vitro study investigates the effects of complex magnetic fields (CMFs) on glial-like cells derived from mesenchymal cell differentiation, serving as a model for neuropathy of the diabetic foot. Through assessments of cellular proliferation, hemocompatibility, mutagenicity, and mitochondrial membrane potential, we have established the safety profile of the system. Furthermore, the analysis of microRNAs (miRNAs) suggests that CMFs may exert beneficial effects on cell cycle regulation, as evidenced by the upregulation of the miRNAs within the 121, 127, and 142 families, which are known to be associated with mitochondrial function and cell cycle control. This exploration holds promise for potential applications in mitigating neuropathic complications in diabetic foot conditions.