Two-dimensional electrophoresis analysis of proteomics based on image feature and mathematical morphology

• Published in: Science China. Chemistry Vol. 48; no. 4; pp. 361 - 367
• Main Author: SHEN, Peng
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer Nature B.V 01.08.2005; The First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China%Pharmaceutical Informatics Institute,Zhejiang University,Hangzhou 310027,China
• Subjects: E coli; Electrophoresis; Feature extraction; Mathematical morphology; Proteins; Proteomics; Two dimensional analysis; 2-dimensional electrophoresis; morphological feature; proteomics; analytical chemistry for life; image recognition; protein spot recognition

In this paper, a novel method to automatically detect protein spots on a two-dimensional (2-D) electrophoresis gel image is proposed to implement proteomics analysis of complex analyte. On the basis of the identifying spots results based on color variation and spot size features, morphological featu...