The role of gut microbiota (GM) and GM-related metabolites in diabetes and obesity. A review of analytical methods used to measure GM-related metabolites in fecal samples with a focus on metabolites’ derivatization step

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 191; p. 113617
• Main Authors: Mojsak, Patrycja, Rey-Stolle, Fernanda, Parfieniuk, Ewa, Kretowski, Adam, Ciborowski, Michal
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.11.2020
• Subjects: Derivatization; Diabetes; GC–MS; LC–MS; Microbiota; Obesity; Val; 2-BAP; BcA; HFD; MTBE; TFA; IsCF; BACs; CarA; (NH4)2SO4/NaH2PO4; 3mPA; GC×GC–MS; Gly; CAR/PDMS; FAs; HPLC–UV; GCxGC–TOF/MS; Obesity; LOQs; GC-TOF/MS; IS; PhD; Arg; AcA; RP; TBDMCS; BA; T2DM; MRM; AMBA–d5; AcN; LODs; AAs; O-BHA; DMAP; ESI; 2–PA; CA; HPLC; BMI; CDCA; TBAHS; UHPLC–ESI–MS/MS; IdD; ECF; iBA; AAAs; 4-MVA; MCF; NMR; His; AMBA; LA; Ala; LC; SCFAs; DmPA; Diabetes; 2-NPH; Ile; EDC; MeOX; 2-mBA; MTBSTFA; SPE; Pro; 2-AA; TMCS; PcA; Microbiota; SPME; Trp; AMB; RSDs; HS–SPME; AABD–SH; CID; DCA; HPLC–UV–vis; DHPP; EI; AMQ; MS; GC–FID; LCA; VA; Na2SO4; PCF; BCAAs; MeOH; PFB–Br; DVB/CAR/PDMS; iBcA; DMED; Derivatization; MSTFA; HCl; HS–GC; BQB; OA; CAR/DVB; HS–SPME–GC; iVA; LDL; BSTFA; QuEChERS; GC; 2-mHA; 3mVA; GF; 3–NPH; GC–MS; GM; ILCD; LC–MS; PA; HIQB; Bas; BAT; BCFAs; HA; IPA; TMS; Leu; 4-mHA

[Display omitted] •An association of gut microbiota with type 2 diabetes and obesity was described.•The role of microbial-derived metabolites in diabetes and obesity was assessed.•Methods for fecal metabolites derivatization before GC–MS analysis were reviewed.•Methods for fecal metabolites derivati...