Differential methylation hybridization: profiling DNA methylation with a high-density CpG island microarray

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 507; p. 89
• Main Authors: Yan, Pearlly S, Potter, Dustin, Deatherage, Daniel E, Huang, Tim H-M, Lin, Shili
• Format: Journal Article
• Language: English
• Published: United States 2009
• Subjects: Base Sequence; Biomarkers, Tumor - chemistry; Biomarkers, Tumor - genetics; CpG Islands; Data Interpretation, Statistical; DNA - chemistry; DNA - genetics; DNA Fingerprinting - methods; DNA Fingerprinting - statistics & numerical data; DNA Methylation; DNA, Neoplasm - chemistry; DNA, Neoplasm - genetics; Humans; Neoplasms - chemistry; Neoplasms - genetics; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis - methods; Oligonucleotide Array Sequence Analysis - statistics & numerical data

Differential methylation hybridization (DMH) is a high-throughput DNA methylation screening tool that utilizes methylation-sensitive restriction enzymes to profile methylated fragments by hybridizing them to a CpG island microarray. This array contains probes spanning all the 27,800 islands annotate...