Mouse primary osteoblasts express vitamin D3 25-hydroxylase mRNA and convert 1α-hydroxyvitamin D3 into 1α,25-dihydroxyvitamin D3

• Published in: Bone (New York, N.Y.) Vol. 16; no. 1; pp. 129 - 135
• Main Authors: ICHIKAWA, F, SATO, K, NANJO, M, NISHII, Y, SHINKI, T, TAKAHASHI, N, SUDA, T
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Science 1995
• Subjects: 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - genetics; Animals; Biological and medical sciences; Blotting, Northern; Calcitriol - metabolism; Cells, Cultured; Fundamental and applied biological sciences. Psychology; Hydroxycholecalciferols - metabolism; Mice; Mice, Inbred ICR; Organ Culture Techniques; Organ Specificity - physiology; Osteoblasts - metabolism; RNA, Messenger - biosynthesis; Skeleton and joints; Vertebrates: osteoarticular system, musculoskeletal system; Enzyme; Hydroxylase; Rodentia; Metabolism; Osteoarticular system; Northern blotting; Vertebrata; Mammalia; Enzymatic activity; Mouse; Vitamin D; Animal; Bone; Molecular biology; Colecalciferol

We examined whether 1 alpha-hydroxyvitamin D3 (1 alpha(OH)D3) is metabolized into 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) in bone. Northern blot analysis indicated that the expression of vitamin D3 25-hydroxylase mRNA was highest in the liver, followed by the duodenum, calvaria, lung, kid...