utilization of 5-oxoproline, ammonia and glutamine by Rhizobium leguminosarum in chemostat culture
Shell Research Limited, Sittingbourne Research Centre, Sittingbourne, Kent ME9 8 AG, U.K. Department of Applied Biology, University of Wales Institute of Science and Technology, King Edward VII Avenue, Cardiff CF1 3NU, U.K. ABSTRACT SUMMARY: The growth in continuous culture of Rhizobium leguminosaru...
Saved in:
Published in | Journal of general microbiology Vol. 129; no. 6; pp. 1707 - 1712 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Soc General Microbiol
1983
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Shell Research Limited, Sittingbourne Research Centre, Sittingbourne, Kent ME9 8 AG, U.K.
Department of Applied Biology, University of Wales Institute of Science and Technology, King Edward VII Avenue, Cardiff CF1 3NU, U.K.
ABSTRACT
SUMMARY: The growth in continuous culture of Rhizobium leguminosarum on the nitrogen sources glutamine, ammonia, or ammonia and 5-oxoproline (formed when aqueous glutamine solutions are autoclaved) is described. When growth was nitrogen-limited with autoclaved glutamine the specific rate of 5-oxoproline utilization was independent of dilution rate, whereas the specific rate of ammonia utilization increased with dilution rate. Although the culture was nitrogen-limited, excess nitrogen (as 5-oxoproline) was still present in the supernatant, especially at high dilution rates.
Values obtained for Y max glucose and Y max oxgen were 72.2 and 32.5 g dry wt bacteria (mol substrate) -1 , respectively, under ammonia/5-oxoproline limitation and 95.7 and 30.2 g dry wt bacteria (mol substrate) -1 , respectively, under glutamine limitation.
Present address: Biological Laboratory, University of Kent at Canterbury, Canterbury, Kent CT2 7NJ, U.K.
Present address: Biotechnology Department, Kuwait Institute for Scientific Research, P.O. Box 24885, Safat, Kuwait. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-1287 1350-0872 1465-2080 |
DOI: | 10.1099/00221287-129-6-1707 |