Microfluidic chips for real-time PCR

Abstract The results of real-time PCR in single-chamber microfluidic chips made of silicon-glass materials and optically transparent polymethyl methacrylate are presented. Conditions for efficient thermal cycling in microchip devices with several reaction chambers are discussed. A simplified theoret...

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Published inJournal of physics. Conference series Vol. 2086; no. 1; pp. 12124 - 12127
Main Authors Zubik, A N, Rudnitskaya, G E, Bulyanitsa, A L, Lukashenko, T A, Evstrapov, A A
Format Journal Article
LanguageEnglish
Published IOP Publishing 01.12.2021
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Summary:Abstract The results of real-time PCR in single-chamber microfluidic chips made of silicon-glass materials and optically transparent polymethyl methacrylate are presented. Conditions for efficient thermal cycling in microchip devices with several reaction chambers are discussed. A simplified theoretical estimation of the duration of heating a liquid in a polymer microchip is proposed, the results of which correlate with experimental data.
ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/2086/1/012124