Pluripotent stem cell-derived neural stem cells: From basic research to applications

Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological devel...

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Published inWorld journal of stem cells Vol. 6; no. 5; pp. 651 - 657
Main Authors Otsu, Masahiro, Nakayama, Takashi, Inoue, Nobuo
Format Journal Article
LanguageEnglish
Published United States Baishideng Publishing Group Inc 26.11.2014
Subjects
cells
Embryonic
Minireviews
Neural
Pluripotent
stem
Neural Stem Sphere method
Neural stem cells
Embryonic stem cells
Pluripotent stem cells
Cell-based therapies
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Abstract Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.
AbstractList Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.
Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.
Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.
Author Masahiro Otsu Takashi Nakayama Nobuo Inoue
AuthorAffiliation Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan Department of Biochemistry, Yokohama City University School of Medicine, Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Higashiogu,Arakawa-ku, Tokyo 116-8551, Japan
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Keywords Neural Stem Sphere method
Neural stem cells
Embryonic stem cells
Pluripotent stem cells
Cell-based therapies
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Notes Masahiro Otsu;Takashi Nakayama;Nobuo Inoue;Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan;Department of Biochemistry, Yokohama City University School of Medicine, Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Higashiogu,Arakawa-ku, Tokyo 116-8551, Japan
Correspondence to: Nobuo Inoue, PhD, Laboratory of Regenerative Neurosciences, Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Higashiogu, Arakawa-ku, Tokyo 116-8551, Japan. jun-inoue@bg8.so-net.ne.jp
Author contributions: Otsu M and Inoue N drafted the article; Otsu M wrote this manuscript; Otsu M, Nakayama T and Inoue N revised this manuscript critically for important intellectual content.
Telephone: +81-3-38197382 Fax: +81-3-38191406
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