Metabotropic Glutamate Receptor 1 Internalization Induced by Muscarinic Acetylcholine Receptor Activation: Differential Dependency of Internalization of Splice Variants on Nonvisual Arrestins

• Published in: Molecular pharmacology Vol. 61; no. 5; pp. 1114 - 1123
• Main Authors: Mundell, Stuart J., Matharu, Anne-Lise, Pula, Giordano, Holman, David, Roberts, Peter J., Kelly, Eamonn
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2002
• Subjects: 2-[1-(3-dimethylamino-propyl)indol-3-yl]-3-(1H-indol-3-yl)maleimide; 7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxylate ethyl ester; acetylcholine receptor; AChR; Alternative Splicing - drug effects; Amino Acid Sequence; analysis of variance; Animals; ANOVA; arr-DNM; arrestin-2 dominant-negative mutant; Arrestins - metabolism; bovine serum albumin; BSA; Ca2+-calmodulin-dependent kinase II; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; CaMKII; Cells, Cultured; Cercopithecus aethiops; COS Cells; CPCCOEt; DMEM; Dulbecco’s modified Eagle’s medium; dyn-DNM; dynamin dominant-negative mutant; Dynamins; ELISA; Endocytosis - drug effects; Endocytosis - physiology; enzyme-linked immunosorbent assay; G-protein-coupled receptor; G-protein-coupled receptor kinase; GF 109203X; GFP; Glutamic Acid - pharmacology; GPCR; green fluorescent protein; GRK; GTP Phosphohydrolases - metabolism; HEK; hemagglutinin; human embryonic kidney; Humans; inositol phosphate; kilobase(s); KN-93; metabotropic glutamate receptor; mGluR; Molecular Sequence Data; N-(2-[N-[4-chlorocinnamyl]-N-methylaminomethyl]phenyl)-N-(2-hydroxyethyl)-4-methoxybenzenesulfonamide; PCR; phorbol 12-myristate 13-acetate; PKA; PKC; PMA; polymerase chain reaction; protein kinase A; protein kinase C; Protein Kinase C - metabolism; Receptors, Metabotropic Glutamate - genetics; Receptors, Metabotropic Glutamate - metabolism; Receptors, Muscarinic - metabolism; Second Messenger Systems; Signal Transduction - physiology; TBS; Transfection; Tris-buffered saline; GRK; KN-93; dyn-DNM; TBS; CPCCOEt; PMA; IP; PKA; PKC; mGluR; HEK; GFP; DMEM; BSA; GF 109203X; AChR; CaMKII; ANOVA; kb; arr-DNM; GPCR; HA; PCR; ELISA

In this study, we characterized the glutamate- or second-messenger kinase-dependent internalization of the rat metabotropic glutamate receptor 1 (mGluR1) splice variants 1a, 1b, and 1c, and assessed the arrestin and dynamin dependence of these processes. To facilitate this we inserted a hemagglutini...