Glycomyces tarimensis sp. nov., an actinomycete isolated from a saline-alkali habitat

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. Pt 5; pp. 1587 - 1591
• Main Authors: Lv, Ling-Ling, Zhang, Yue-Feng, Zhang, Li-Li
• Format: Journal Article
• Language: English
• Published: England 01.05.2015
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Alkalies; Base Composition; China; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Molecular Sequence Data; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Salinity; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2 - chemistry
• DOI: 10.1099/ijs.0.000143
• ISSN: 1466-5026

A novel actinomycete strain, designated TRM 45387(T), was isolated from a saline-alkali soil in Xinjiang Province (40° 22' N 79° 08' E), north-west China. The isolate was characterized using a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain TRM 45387(T) belonged to the genus Glycomyces and was closely related to Glycomyces arizonensis DSM 44726(T) (96.59% 16S rRNA gene sequence similarity). The G+C content of the DNA was 71.26 mol%. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid, and xylose, glucose, galactose, arabinose and ribose as the major whole-cell sugars. The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannosides. The predominant menaquinone was MK-10(H6). The major fatty acids were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0. On the basis of the evidence from this polyphasic study, a novel species, Glycomyces tarimensis sp. nov., is proposed. The type strain of Glycomyces tarimensis is TRM 45387(T) ( =CCTCC AA 2014007(T) =JCM 30184(T)).