CHARACTERIZATION AND TOXICITY OF CARBON DOT-POLY(LACTIC-CO-GLYCOLIC ACID) NANOCOMPOSITES FOR BIOMEDICAL IMAGING
Semiconductor quantum dots (QDs) have achieved initial success as biomedical imaging agents. However, significant cytotoxicity in the biological environment prohibits their use in vivo. Here, we introduce nanocomposites composed of carbon dots (C-dots) in poly(lactic-co-glycolic acid) (PLGA) carrier...
Saved in:
Published in | Nano LIFE Vol. 3; no. 1; p. 1340002 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Hackensack
World Scientific Publishing Company
01.03.2013
World Scientific Publishing Co. Pte., Ltd |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Semiconductor quantum dots (QDs) have achieved initial success as biomedical imaging agents. However, significant cytotoxicity in the biological environment prohibits their use in vivo. Here, we introduce nanocomposites composed of carbon dots (C-dots) in poly(lactic-co-glycolic acid) (PLGA) carriers as possible imaging agents for in vivo applications. An initial hurdle to clinical use is overcome by synthesizing C-dots with commercially available carbon black precursors, permitting scalable nanomanufacturing. These fluorescent nanoparticles, which have a mean diameter of ~1 nm, display a disordered graphitic structure. To overcome a second clinical hurdle (i.e., rapid renal clearance of nanoparticles <~6 nm in diameter), C-dots were encapsulated in biodegradable PLGA nanospheres. The resulting nanocomposites showed a mean diameter of 344 ± 23 nm, which should reduce renal clearance. With further optimization, nanocarriers could be optimized to sizes <200 nm to reduce accumulation in the reticuloendothelial system (RES). Toxicity of both C-dots and C-dot-PLGA nanocomposites was evaluated using HepG2 liver cell lines. Unlike QDs, which can induce toxicological responses at concentrations as low as 0.005 mg/mL, C-dots exhibited cytotoxicity at concentrations greater than 0.2 mg/mL, while their derived nanocomposites did not exhibit cytotoxicity at any concentration tested (i.e., 0.02 mg/mL, 0.1 mg/mL and 0.2 mg/mL). |
---|---|
AbstractList | Semiconductor quantum dots (QDs) have achieved initial success as biomedical imaging agents. However, significant cytotoxicity in the biological environment prohibits their use in vivo. Here, we introduce nanocomposites composed of carbon dots (C-dots) in poly(lactic-co-glycolic acid) (PLGA) carriers as possible imaging agents for in vivo applications. An initial hurdle to clinical use is overcome by synthesizing C-dots with commercially available carbon black precursors, permitting scalable nanomanufacturing. These fluorescent nanoparticles, which have a mean diameter of ~1 nm, display a disordered graphitic structure. To overcome a second clinical hurdle (i.e., rapid renal clearance of nanoparticles <~6 nm in diameter), C-dots were encapsulated in biodegradable PLGA nanospheres. The resulting nanocomposites showed a mean diameter of 344 ± 23 nm, which should reduce renal clearance. With further optimization, nanocarriers could be optimized to sizes <200 nm to reduce accumulation in the reticuloendothelial system (RES). Toxicity of both C-dots and C-dot-PLGA nanocomposites was evaluated using HepG2 liver cell lines. Unlike QDs, which can induce toxicological responses at concentrations as low as 0.005 mg/mL, C-dots exhibited cytotoxicity at concentrations greater than 0.2 mg/mL, while their derived nanocomposites did not exhibit cytotoxicity at any concentration tested (i.e., 0.02 mg/mL, 0.1 mg/mL and 0.2 mg/mL). |
Author | WETTA, OLIVIA G. WINTER, JESSICA O. OLESIK, KRISTI M. DORCÉNA, C. JENNY |
Author_xml | – sequence: 1 givenname: C. JENNY surname: DORCÉNA fullname: DORCÉNA, C. JENNY email: dorcena.1@osu.edu organization: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave, Columbus, OH 43210, USA – sequence: 2 givenname: KRISTI M. surname: OLESIK fullname: OLESIK, KRISTI M. email: olesik.3@osu.edu organization: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave, Columbus, OH 43210, USA – sequence: 3 givenname: OLIVIA G. surname: WETTA fullname: WETTA, OLIVIA G. email: wetta.4@osu.edu organization: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave, Columbus, OH 43210, USA – sequence: 4 givenname: JESSICA O. surname: WINTER fullname: WINTER, JESSICA O. email: winter.63@osu.edu organization: William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave, Columbus, OH 43210, USA |
BookMark | eNplkF1rwjAUhsNwMOf8AbsL7Ga76Jacxia9jKkfgdqIdjB3UzRNQXHWtcrYv1_F4Y1X5_DyPOfAe49au3LnEHqk5JVSBm9zykM_FIxRnxFCwL9B7VPkhaIHrcvO2B3q1vWmQUgvAM5FG5VqLGdSpYOZ_pSpNgmWSYRT86GVThfYDLGSs34TRyb1piZePMcNrZWnjDeKF8rEWmGpdPSCE5kYZSZTM9fpYI6HZob72kwGkVYyxnoiRzoZPaDbYrmtXfd_dtD7cJCqsReb0YnzLDDme35YcAcA1tqVWzkKhCyJE7nNIYBVIAJuizy0QALBnQiZW0LOOS0ch5wFgvgd9HS-u6_K76OrD9mmPFa75mUGwAK_BywUDUXPlK3Kuq5cke2r9dey-s0oyU7VZlfVNg45Oz9ltc1ru3a7w7pY24t6rfwBsLRybg |
CitedBy_id | crossref_primary_10_1007_s12217_016_9533_6 crossref_primary_10_1016_j_biocel_2014_11_016 crossref_primary_10_1142_S1793984413020017 crossref_primary_10_1016_j_bbamem_2021_183817 crossref_primary_10_1039_D0TB01334J crossref_primary_10_1021_acs_jchemed_6b00995 crossref_primary_10_1002_biot_201300038 crossref_primary_10_1021_acsbiomaterials_3c00918 crossref_primary_10_1039_D3NR01424J crossref_primary_10_1016_j_matchemphys_2019_03_060 |
Cites_doi | 10.1021/nn900546b 10.1021/la060093j 10.1016/j.jpcs.2011.03.006 10.1039/b812420e 10.1021/nl047996m 10.1142/S1793984410000158 10.1021/ja073527l 10.1002/wnan.66 10.1021/ja904843x 10.1039/c0jm02963g 10.1021/jp905912n 10.1163/156856206775997322 10.1021/nl0347334 10.1002/anie.200906623 10.1021/cm900709w 10.1289/ehp.7339 10.1021/jp9085969 10.1039/b907612c 10.1021/es800254a 10.1016/S0168-3659(96)01484-8 10.1007/s00109-007-0274-2 10.1016/j.chembiol.2005.09.008 10.1016/j.mrfmmm.2003.07.011 10.1093/toxsci/kfg228 10.1529/biophysj.107.108134 10.1038/nbt1340 10.1021/ja062677d 10.1016/j.saa.2009.11.015 10.1021/cm901593y 10.1007/s00109-004-0629-x 10.1002/anie.200701271 10.3109/03639049209069315 10.1289/ehp.11566 10.1016/j.carbon.2005.02.018 10.1073/pnas.1018382108 10.1021/jp8076485 |
ContentType | Journal Article |
Copyright | 2013, World Scientific Publishing Company 2013. World Scientific Publishing Company |
Copyright_xml | – notice: 2013, World Scientific Publishing Company – notice: 2013. World Scientific Publishing Company |
DBID | AAYXX CITATION 7QO 8BQ 8FD FR3 JG9 P64 |
DOI | 10.1142/S1793984413400023 |
DatabaseName | CrossRef Biotechnology Research Abstracts METADEX Technology Research Database Engineering Research Database Materials Research Database Biotechnology and BioEngineering Abstracts |
DatabaseTitle | CrossRef Materials Research Database Engineering Research Database Biotechnology Research Abstracts Technology Research Database METADEX Biotechnology and BioEngineering Abstracts |
DatabaseTitleList | CrossRef Materials Research Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
EISSN | 1793-9852 |
ExternalDocumentID | 10_1142_S1793984413400023 S1793984413400023 |
GroupedDBID | 0R~ 4.4 ABDBF ADSJI ALMA_UNASSIGNED_HOLDINGS EBS EJD EOJEC ESX HZ~ O9- OBODZ OK1 RWJ AAYXX CITATION 7QO 8BQ 8FD FR3 JG9 P64 |
ID | FETCH-LOGICAL-c2443-39f7e222cccbebe1200a0e8dcd262b6867cfd9c20687e894ea2d771fe72d46803 |
ISSN | 1793-9844 |
IngestDate | Sat Aug 31 00:18:32 EDT 2024 Fri Aug 23 00:46:53 EDT 2024 Fri Aug 23 08:19:58 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | quantum dots carbon nanocomposites Carbon dots cytotoxicity |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c2443-39f7e222cccbebe1200a0e8dcd262b6867cfd9c20687e894ea2d771fe72d46803 |
PQID | 2246352498 |
PQPubID | 2069558 |
ParticipantIDs | proquest_journals_2246352498 crossref_primary_10_1142_S1793984413400023 worldscientific_primary_S1793984413400023 |
PublicationCentury | 2000 |
PublicationDate | 20130300 2013-03-00 20130301 |
PublicationDateYYYYMMDD | 2013-03-01 |
PublicationDate_xml | – month: 03 year: 2013 text: 20130300 |
PublicationDecade | 2010 |
PublicationPlace | Hackensack |
PublicationPlace_xml | – name: Hackensack |
PublicationTitle | Nano LIFE |
PublicationYear | 2013 |
Publisher | World Scientific Publishing Company World Scientific Publishing Co. Pte., Ltd |
Publisher_xml | – name: World Scientific Publishing Company – name: World Scientific Publishing Co. Pte., Ltd |
References | rf5 rf23 rf4 rf22 rf7 rf25 rf6 rf24 rf9 rf8 rf21 rf20 rf27 rf26 rf29 rf28 rf12 rf34 rf11 rf33 rf14 rf36 rf13 rf35 rf30 rf10 rf32 rf19 rf16 Ruan G. (rf31) 2010; 223 rf15 rf37 rf18 rf17 rf1 rf3 rf2 |
References_xml | – ident: rf15 doi: 10.1021/nn900546b – ident: rf37 doi: 10.1021/la060093j – ident: rf24 doi: 10.1016/j.jpcs.2011.03.006 – ident: rf9 doi: 10.1039/b812420e – ident: rf6 doi: 10.1021/nl047996m – ident: rf11 doi: 10.1142/S1793984410000158 – ident: rf14 doi: 10.1021/ja073527l – ident: rf32 doi: 10.1002/wnan.66 – ident: rf16 doi: 10.1021/ja904843x – ident: rf22 doi: 10.1039/c0jm02963g – ident: rf18 doi: 10.1021/jp905912n – ident: rf33 doi: 10.1163/156856206775997322 – ident: rf1 doi: 10.1021/nl0347334 – ident: rf30 doi: 10.1002/anie.200906623 – ident: rf19 doi: 10.1021/cm900709w – ident: rf27 doi: 10.1289/ehp.7339 – ident: rf10 doi: 10.1021/jp9085969 – ident: rf21 doi: 10.1039/b907612c – ident: rf4 doi: 10.1021/es800254a – ident: rf35 doi: 10.1016/S0168-3659(96)01484-8 – ident: rf5 doi: 10.1007/s00109-007-0274-2 – volume: 223 start-page: 81 year: 2010 ident: rf31 publication-title: J. Nanoeng. Nanosyst. contributor: fullname: Ruan G. – ident: rf3 doi: 10.1016/j.chembiol.2005.09.008 – ident: rf2 doi: 10.1016/j.mrfmmm.2003.07.011 – ident: rf26 doi: 10.1093/toxsci/kfg228 – ident: rf8 doi: 10.1529/biophysj.107.108134 – ident: rf23 doi: 10.1038/nbt1340 – ident: rf13 doi: 10.1021/ja062677d – ident: rf29 doi: 10.1016/j.saa.2009.11.015 – ident: rf20 doi: 10.1021/cm901593y – ident: rf36 doi: 10.1007/s00109-004-0629-x – ident: rf17 doi: 10.1002/anie.200701271 – ident: rf34 doi: 10.3109/03639049209069315 – ident: rf7 doi: 10.1289/ehp.11566 – ident: rf28 doi: 10.1016/j.carbon.2005.02.018 – ident: rf25 doi: 10.1073/pnas.1018382108 – ident: rf12 doi: 10.1021/jp8076485 |
SSID | ssj0000562778 |
Score | 1.9861461 |
Snippet | Semiconductor quantum dots (QDs) have achieved initial success as biomedical imaging agents. However, significant cytotoxicity in the biological environment... |
SourceID | proquest crossref worldscientific |
SourceType | Aggregation Database Publisher |
StartPage | 1340002 |
SubjectTerms | Biocompatibility Biodegradability Biodegradation Biomedical materials Black carbon Carbon Carbon black Carbon dots Cell lines Cytotoxicity Fluorescence Glycolic acid Graphitic structure Hepatocytes Medical imaging Nanocomposites Nanoparticles Nanospheres Optimization Polylactide-co-glycolide Quantum dots Reticuloendothelial system Toxicity testing |
Title | CHARACTERIZATION AND TOXICITY OF CARBON DOT-POLY(LACTIC-CO-GLYCOLIC ACID) NANOCOMPOSITES FOR BIOMEDICAL IMAGING |
URI | http://www.worldscientific.com/doi/abs/10.1142/S1793984413400023 https://www.proquest.com/docview/2246352498/abstract/ |
Volume | 3 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj5swELbS3Ut7WPWppt1WPvTQLSIlYLA5EufFNsGrhK2SvUQBzDGputlLf0l_bse8E3ro9oIiBwzyfB7PjMffIPSJ9CNYhFkEvonBdKLyG93IsHWHRY50U-mSVO3ozgNnekuuV_aq0_ndyFp6OES9-Ndfz5X8j1ShDeSqTsk-QrJVp9AAv0G-cAUJw_WfZMyn3sLjYJD6d3n1HMUVFYqVz_1wrfJ5uLcYQPNQhPqNmK1V-Q6VNcJ1LvTJbM3FzM8qWQ9VcCDwAsHF_EYsQZ0tNXAPtYEvSv5Hf-5NSvapwpgFzbwH7TeuKQvEgqudd-YGXhaB7WnXoyCorGUxGy39b5luWfjL0NfmvWpRGIVh9gx80nff0yb1Pxlrb84KCqqfe5roNWMVqm6E1YxV5PlBmdLKEqGaobaW-lOaQ3dZzg7Zk802-0h_W6cwba8KRLHMLtXTqsO-RTKen3oJrBITW_c8QecmdW1w68-9wXAwruJ3ynCk2UJffWixaQ5v-9rq59jsqX2Zi4wY974akoZxEz5HF4VXgr0cYi9QR-5eomcNrspXaH8KNgxgwyXYsBjjHGy4BNvnNtSwgtoVPgYaBqDhGmi4ANprdDsehXyqF9U69BhMREu33JRKsDbjOI5AM_Rhom4NyZI4MR0zcphD4zRxY9NwGJXMJXJrJpT2U0nNhDjMsN6gs91-J98izOytRW2HGKlFSSplpNizLbo1oziJUyvtoi_lYG5-5KQsm_yAvblpjXwXXZbDvSnm7v1G0SiC60Fc1kVXJyKoumx19e4R975HT-sZcInODj8f5AewXw_RxwJKfwCPzHx2 |
link.rule.ids | 315,786,790,27957,27958 |
linkProvider | EBSCOhost |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=CHARACTERIZATION+AND+TOXICITY+OF+CARBON+DOT-POLY%28LACTIC-CO-GLYCOLIC+ACID%29+NANOCOMPOSITES+FOR+BIOMEDICAL+IMAGING&rft.jtitle=Nano+LIFE&rft.au=DORC%C3%89NA%2C+C.+JENNY&rft.au=OLESIK%2C+KRISTI+M.&rft.au=WETTA%2C+OLIVIA+G.&rft.au=WINTER%2C+JESSICA+O.&rft.date=2013-03-01&rft.pub=World+Scientific+Publishing+Company&rft.issn=1793-9844&rft.eissn=1793-9852&rft.volume=3&rft.issue=1&rft_id=info:doi/10.1142%2FS1793984413400023&rft.externalDocID=S1793984413400023 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1793-9844&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1793-9844&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1793-9844&client=summon |