Radioimmunoassay of unconjugated and total serum estetrol using a 125I-iodinated tracer

• Published in: Hormone research Vol. 11; no. 5; p. 227
• Main Authors: Zucchelli, G C, Giannessi, D, Piro, M A, Ferdeghini, M, Malvano, R
• Format: Journal Article
• Language: English
• Published: Switzerland 1979
• Subjects: Circadian Rhythm; Cross Reactions; Estetrol - blood; Estriol - analogs & derivatives; Female; Humans; Iodine Radioisotopes; Male; Pregnancy; Radioimmunoassay - methods

A radioimmunossay (RIA) for the measurement of both unconjugated and total serum estetrol has been developed, using an antiserum to an E4-3-conjugate and a 125I-radioiodinated E4 tracer. Assay of dried ethyl ether extracts was used for the determination of unconjugated E4, while a direct measurement...