Detection and characterisation of a sixth Candida auris clade in Singapore: a genomic and phenotypic study
The emerging fungal pathogen Candida auris poses a serious threat to global public health due to its worldwide distribution, multidrug resistance, high transmissibility, propensity to cause outbreaks, and high mortality. We aimed to characterise three unusual C auris isolates detected in Singapore,...
Saved in:
Published in | The Lancet. Microbe Vol. 5; no. 9; p. 100878 |
---|---|
Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.09.2024
|
Online Access | Get full text |
Cover
Loading…
Abstract | The emerging fungal pathogen Candida auris poses a serious threat to global public health due to its worldwide distribution, multidrug resistance, high transmissibility, propensity to cause outbreaks, and high mortality. We aimed to characterise three unusual C auris isolates detected in Singapore, and to determine whether they constitute a novel clade distinct from all previously known C auris clades (I–V).
In this genotypic and phenotypic study, we characterised three C auris clinical isolates, which were cultured from epidemiologically unlinked inpatients at a large tertiary hospital in Singapore. The index isolate was detected in April, 2023. We performed whole-genome sequencing (WGS) and obtained hybrid assemblies of these C auris isolates. The complete genomes were compared with representative genomes of all known C auris clades. To provide a global context, 3651 international WGS data from the National Center for Biotechnology Information (NCBI) database were included in a high-resolution single nucleotide polymorphism (SNP) analysis. Antifungal susceptibility testing was done and antifungal resistance genes, mating-type locus, and chromosomal rearrangements were characterised from the WGS data of the three investigated isolates. We further implemented Bayesian logistic regression models to classify isolates into known clades and simulate the automatic detection of isolates belonging to novel clades as their WGS data became available.
The three investigated isolates were separated by at least 37 000 SNPs (range 37 000–236 900) from all existing C auris clades. These isolates had opposite mating-type allele and different chromosomal rearrangements when compared with their closest clade IV relatives. The isolates were susceptible to all tested antifungals. Therefore, we propose that these isolates represent a new clade of C auris, clade VI. Furthermore, an independent WGS dataset from Bangladesh, accessed via the NCBI Sequence Read Archive, was found to belong to this new clade. As a proof-of-concept, our Bayesian logistic regression model was able to flag these outlier genomes as a potential new clade.
The discovery of a new C auris clade in Singapore and Bangladesh in the Indomalayan zone, showing a close relationship to clade IV members most commonly found in South America, highlights the unknown genetic diversity and origin of C auris, particularly in under-resourced regions. Active surveillance in clinical settings, along with effective sequencing strategies and downstream analysis, will be essential in the identification of novel strains, tracking of transmission, and containment of adverse clinical effects of C auris infections.
Duke-NUS Academic Medical Center Nurturing Clinician Researcher Scheme, and the Genedant-GIS Innovation Program. |
---|---|
AbstractList | The emerging fungal pathogen Candida auris poses a serious threat to global public health due to its worldwide distribution, multidrug resistance, high transmissibility, propensity to cause outbreaks, and high mortality. We aimed to characterise three unusual C auris isolates detected in Singapore, and to determine whether they constitute a novel clade distinct from all previously known C auris clades (I-V).BACKGROUNDThe emerging fungal pathogen Candida auris poses a serious threat to global public health due to its worldwide distribution, multidrug resistance, high transmissibility, propensity to cause outbreaks, and high mortality. We aimed to characterise three unusual C auris isolates detected in Singapore, and to determine whether they constitute a novel clade distinct from all previously known C auris clades (I-V).In this genotypic and phenotypic study, we characterised three C auris clinical isolates, which were cultured from epidemiologically unlinked inpatients at a large tertiary hospital in Singapore. The index isolate was detected in April, 2023. We performed whole-genome sequencing (WGS) and obtained hybrid assemblies of these C auris isolates. The complete genomes were compared with representative genomes of all known C auris clades. To provide a global context, 3651 international WGS data from the National Center for Biotechnology Information (NCBI) database were included in a high-resolution single nucleotide polymorphism (SNP) analysis. Antifungal susceptibility testing was done and antifungal resistance genes, mating-type locus, and chromosomal rearrangements were characterised from the WGS data of the three investigated isolates. We further implemented Bayesian logistic regression models to classify isolates into known clades and simulate the automatic detection of isolates belonging to novel clades as their WGS data became available.METHODSIn this genotypic and phenotypic study, we characterised three C auris clinical isolates, which were cultured from epidemiologically unlinked inpatients at a large tertiary hospital in Singapore. The index isolate was detected in April, 2023. We performed whole-genome sequencing (WGS) and obtained hybrid assemblies of these C auris isolates. The complete genomes were compared with representative genomes of all known C auris clades. To provide a global context, 3651 international WGS data from the National Center for Biotechnology Information (NCBI) database were included in a high-resolution single nucleotide polymorphism (SNP) analysis. Antifungal susceptibility testing was done and antifungal resistance genes, mating-type locus, and chromosomal rearrangements were characterised from the WGS data of the three investigated isolates. We further implemented Bayesian logistic regression models to classify isolates into known clades and simulate the automatic detection of isolates belonging to novel clades as their WGS data became available.The three investigated isolates were separated by at least 37 000 SNPs (range 37 000-236 900) from all existing C auris clades. These isolates had opposite mating-type allele and different chromosomal rearrangements when compared with their closest clade IV relatives. The isolates were susceptible to all tested antifungals. Therefore, we propose that these isolates represent a new clade of C auris, clade VI. Furthermore, an independent WGS dataset from Bangladesh, accessed via the NCBI Sequence Read Archive, was found to belong to this new clade. As a proof-of-concept, our Bayesian logistic regression model was able to flag these outlier genomes as a potential new clade.FINDINGSThe three investigated isolates were separated by at least 37 000 SNPs (range 37 000-236 900) from all existing C auris clades. These isolates had opposite mating-type allele and different chromosomal rearrangements when compared with their closest clade IV relatives. The isolates were susceptible to all tested antifungals. Therefore, we propose that these isolates represent a new clade of C auris, clade VI. Furthermore, an independent WGS dataset from Bangladesh, accessed via the NCBI Sequence Read Archive, was found to belong to this new clade. As a proof-of-concept, our Bayesian logistic regression model was able to flag these outlier genomes as a potential new clade.The discovery of a new C auris clade in Singapore and Bangladesh in the Indomalayan zone, showing a close relationship to clade IV members most commonly found in South America, highlights the unknown genetic diversity and origin of C auris, particularly in under-resourced regions. Active surveillance in clinical settings, along with effective sequencing strategies and downstream analysis, will be essential in the identification of novel strains, tracking of transmission, and containment of adverse clinical effects of C auris infections.INTERPRETATIONThe discovery of a new C auris clade in Singapore and Bangladesh in the Indomalayan zone, showing a close relationship to clade IV members most commonly found in South America, highlights the unknown genetic diversity and origin of C auris, particularly in under-resourced regions. Active surveillance in clinical settings, along with effective sequencing strategies and downstream analysis, will be essential in the identification of novel strains, tracking of transmission, and containment of adverse clinical effects of C auris infections.Duke-NUS Academic Medical Center Nurturing Clinician Researcher Scheme, and the Genedant-GIS Innovation Program.FUNDINGDuke-NUS Academic Medical Center Nurturing Clinician Researcher Scheme, and the Genedant-GIS Innovation Program. The emerging fungal pathogen Candida auris poses a serious threat to global public health due to its worldwide distribution, multidrug resistance, high transmissibility, propensity to cause outbreaks, and high mortality. We aimed to characterise three unusual C auris isolates detected in Singapore, and to determine whether they constitute a novel clade distinct from all previously known C auris clades (I–V). In this genotypic and phenotypic study, we characterised three C auris clinical isolates, which were cultured from epidemiologically unlinked inpatients at a large tertiary hospital in Singapore. The index isolate was detected in April, 2023. We performed whole-genome sequencing (WGS) and obtained hybrid assemblies of these C auris isolates. The complete genomes were compared with representative genomes of all known C auris clades. To provide a global context, 3651 international WGS data from the National Center for Biotechnology Information (NCBI) database were included in a high-resolution single nucleotide polymorphism (SNP) analysis. Antifungal susceptibility testing was done and antifungal resistance genes, mating-type locus, and chromosomal rearrangements were characterised from the WGS data of the three investigated isolates. We further implemented Bayesian logistic regression models to classify isolates into known clades and simulate the automatic detection of isolates belonging to novel clades as their WGS data became available. The three investigated isolates were separated by at least 37 000 SNPs (range 37 000–236 900) from all existing C auris clades. These isolates had opposite mating-type allele and different chromosomal rearrangements when compared with their closest clade IV relatives. The isolates were susceptible to all tested antifungals. Therefore, we propose that these isolates represent a new clade of C auris, clade VI. Furthermore, an independent WGS dataset from Bangladesh, accessed via the NCBI Sequence Read Archive, was found to belong to this new clade. As a proof-of-concept, our Bayesian logistic regression model was able to flag these outlier genomes as a potential new clade. The discovery of a new C auris clade in Singapore and Bangladesh in the Indomalayan zone, showing a close relationship to clade IV members most commonly found in South America, highlights the unknown genetic diversity and origin of C auris, particularly in under-resourced regions. Active surveillance in clinical settings, along with effective sequencing strategies and downstream analysis, will be essential in the identification of novel strains, tracking of transmission, and containment of adverse clinical effects of C auris infections. Duke-NUS Academic Medical Center Nurturing Clinician Researcher Scheme, and the Genedant-GIS Innovation Program. |
ArticleNumber | 100878 |
Author | Spruijtenburg, Bram Tan, Mei Gie Nagarajan, Niranjan Cherng, Benjamin Pei Zhi Meis, Jacques F Boonsimma, Patipan Ling, Moi Lin Tan, Kwee Yuen Lim, Kar Mun Chu, Joash Jun Keat Yang, Yong Chan, Kian Sing Koh, Tse Hsien Venkatachalam, Indumathi Ko, Karrie Kwan Ki Tsui, Clement Kin Ming Tan, Ai Ling Sim, Jean Xiang Ying Aung, May Kyawt Tan, Yen Ee Tan, Ban Hock Shaik Ismail, Bushra Binte Wijaya, Limin Lee, Lai Chee Rajandran, Prevena Oon, Lynette Lin Ean Suphavilai, Chayaporn Goh, Sui Sin |
Author_xml | – sequence: 1 givenname: Chayaporn orcidid: 0000-0002-4777-4586 surname: Suphavilai fullname: Suphavilai, Chayaporn organization: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore – sequence: 2 givenname: Karrie Kwan Ki orcidid: 0000-0002-5968-1870 surname: Ko fullname: Ko, Karrie Kwan Ki email: karrie.ko.k.k@singhealth.com.sg organization: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore – sequence: 3 givenname: Kar Mun surname: Lim fullname: Lim, Kar Mun organization: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore – sequence: 4 givenname: Mei Gie surname: Tan fullname: Tan, Mei Gie organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 5 givenname: Patipan orcidid: 0009-0006-8552-9625 surname: Boonsimma fullname: Boonsimma, Patipan organization: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore – sequence: 6 givenname: Joash Jun Keat orcidid: 0000-0002-0418-245X surname: Chu fullname: Chu, Joash Jun Keat organization: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore – sequence: 7 givenname: Sui Sin surname: Goh fullname: Goh, Sui Sin organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 8 givenname: Prevena surname: Rajandran fullname: Rajandran, Prevena organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 9 givenname: Lai Chee surname: Lee fullname: Lee, Lai Chee organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 10 givenname: Kwee Yuen surname: Tan fullname: Tan, Kwee Yuen organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 11 givenname: Bushra Binte surname: Shaik Ismail fullname: Shaik Ismail, Bushra Binte organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 12 givenname: May Kyawt surname: Aung fullname: Aung, May Kyawt organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 13 givenname: Yong surname: Yang fullname: Yang, Yong organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 14 givenname: Jean Xiang Ying surname: Sim fullname: Sim, Jean Xiang Ying organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 15 givenname: Indumathi surname: Venkatachalam fullname: Venkatachalam, Indumathi organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 16 givenname: Benjamin Pei Zhi surname: Cherng fullname: Cherng, Benjamin Pei Zhi organization: Department of Infectious Diseases, Singapore General Hospital, Singapore – sequence: 17 givenname: Bram orcidid: 0000-0002-6734-361X surname: Spruijtenburg fullname: Spruijtenburg, Bram organization: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, Netherlands – sequence: 18 givenname: Kian Sing surname: Chan fullname: Chan, Kian Sing organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 19 givenname: Lynette Lin Ean surname: Oon fullname: Oon, Lynette Lin Ean organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 20 givenname: Ai Ling surname: Tan fullname: Tan, Ai Ling organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 21 givenname: Yen Ee surname: Tan fullname: Tan, Yen Ee organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 22 givenname: Limin surname: Wijaya fullname: Wijaya, Limin organization: Department of Infectious Diseases, Singapore General Hospital, Singapore – sequence: 23 givenname: Ban Hock surname: Tan fullname: Tan, Ban Hock organization: Department of Infectious Diseases, Singapore General Hospital, Singapore – sequence: 24 givenname: Moi Lin surname: Ling fullname: Ling, Moi Lin organization: Department of Infection Prevention and Epidemiology, Singapore General Hospital, Singapore – sequence: 25 givenname: Tse Hsien surname: Koh fullname: Koh, Tse Hsien organization: Department of Microbiology, Singapore General Hospital, Singapore – sequence: 26 givenname: Jacques F orcidid: 0000-0003-3253-6080 surname: Meis fullname: Meis, Jacques F organization: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, Netherlands – sequence: 27 givenname: Clement Kin Ming orcidid: 0000-0001-5129-1037 surname: Tsui fullname: Tsui, Clement Kin Ming organization: Infectious Diseases Research Laboratory, National Centre for Infectious Diseases, Tan Tock Seng Hospital, Singapore – sequence: 28 givenname: Niranjan surname: Nagarajan fullname: Nagarajan, Niranjan organization: Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39008997$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkE1PGzEQhq2KCijlJ4B8pIdt_R2bC0Lpp4TUA-Vsee1ZYpTYi71bNf--JqGIW0_2vH5mRn7eoYOUEyB0RslHSqj6dMuUUp1kYnHBxAdCWtiRN-j4JT54dT9Cp7U-EEKYpIxKeYiOuCFEG7M4Rg-fYQI_xZywSwH7lSvOT1BidbswD9jhGv9MK7xsQAwOu7m9Yr92AXBM-DamezfmApeNvIeUN9HvZo2rVkzbsZV1msP2PXo7uHWF0-fzBN19_fJr-b27-fntx_L6pvNM8KkTvTLGBS60GIRkhnileueJ4g56riXnVBFm6MIx4U0guvfcS-5MD4Myuucn6GI_dyz5cYY62U2sHtZrlyDP1XKiKdNaU9lQuUd9ybUWGOxY4saVraXEPpm2O9P2SaNlwu5MW9L6zp9XzP0GwkvXP68NuNoD0D76O0Kx1UdIHkIsTbcNOf5nxV-LBY8G |
Cites_doi | 10.1128/JCM.01624-18 10.1093/cid/ciw691 10.1016/j.csbj.2022.06.030 10.3201/eid2301.161497 10.1111/ajt.16144 10.1186/s13059-014-0524-x 10.1080/21505594.2021.1908765 10.1186/s13756-016-0132-5 10.1101/gr.094052.109 10.3201/eid2509.190686 10.1128/spectrum.02645-22 10.7326/M22-3469 10.1016/S1473-3099(18)30597-8 10.1016/j.cmi.2023.04.025 10.1128/aac.02242-21 10.1056/NEJMoa1714373 10.1038/s41564-022-01089-w 10.1016/j.ijantimicag.2019.09.016 10.1093/genetics/iyab029 10.1016/j.jiac.2019.05.034 10.1093/jac/dkx480 10.2807/1560-7917.ES.2022.27.46.2200846 10.1038/s41579-023-00861-x 10.1128/mBio.03364-19 10.1128/JCM.00921-17 10.1111/myc.12781 10.1080/22221751.2022.2125349 10.3201/eid2410.180649 10.1038/s41467-018-07779-6 |
ContentType | Journal Article |
Copyright | 2024 The Author(s) Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved. |
Copyright_xml | – notice: 2024 The Author(s) – notice: Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved. |
DBID | 6I. AAFTH NPM AAYXX CITATION 7X8 |
DOI | 10.1016/S2666-5247(24)00101-0 |
DatabaseName | ScienceDirect Open Access Titles Elsevier:ScienceDirect:Open Access PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic PubMed |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 2666-5247 |
ExternalDocumentID | 10_1016_S2666_5247_24_00101_0 39008997 S2666524724001010 |
Genre | Journal Article |
GrantInformation | Duke-NUS Academic Medical Center Nurturing Clinician Researcher Scheme, and the Genedant-GIS Innovation Program. |
GroupedDBID | .1- .FO 0R~ 0SF 6I. AAEDW AAFTH AALRI AAXUO AFRHN AITUG AJUYK AKRWK ALMA_UNASSIGNED_HOLDINGS EBS FDB GROUPED_DOAJ M41 M~E NCXOZ OK1 ROL Z5R AAMRU NPM 53G AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c243t-4b699ad3484f45290c66bac063aeb385331602917a24c9d08bc3c53a9bef698b3 |
ISSN | 2666-5247 |
IngestDate | Thu Dec 05 22:30:52 EST 2024 Fri Nov 22 02:28:41 EST 2024 Sat Nov 02 12:27:18 EDT 2024 Sat Sep 07 15:50:49 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 9 |
Language | English |
License | This is an open access article under the CC BY license. Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved. |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c243t-4b699ad3484f45290c66bac063aeb385331602917a24c9d08bc3c53a9bef698b3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ORCID | 0009-0006-8552-9625 0000-0003-3253-6080 0000-0002-6734-361X 0000-0002-4777-4586 0000-0001-5129-1037 0000-0002-5968-1870 0000-0002-0418-245X |
OpenAccessLink | http://dx.doi.org/10.1016/S2666-5247(24)00101-0 |
PMID | 39008997 |
PQID | 3081288815 |
PQPubID | 23479 |
ParticipantIDs | proquest_miscellaneous_3081288815 crossref_primary_10_1016_S2666_5247_24_00101_0 pubmed_primary_39008997 elsevier_sciencedirect_doi_10_1016_S2666_5247_24_00101_0 |
PublicationCentury | 2000 |
PublicationDate | 2024-09-01 |
PublicationDateYYYYMMDD | 2024-09-01 |
PublicationDate_xml | – month: 09 year: 2024 text: 2024-09-01 day: 01 |
PublicationDecade | 2020 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | The Lancet. Microbe |
PublicationTitleAlternate | Lancet Microbe |
PublicationYear | 2024 |
Publisher | Elsevier Ltd |
Publisher_xml | – name: Elsevier Ltd |
References | Adams, Quinn, Tsay (bib3) 2018; 24 Chow, Muñoz, Gade (bib26) 2020; 11 Fisher, Denning (bib9) 2023; 21 Hernando-Ortiz, Mateo, Perez-Rodriguez, de Groot, Quindós, Eraso (bib16) 2021; 12 Chow, Gade, Tsay (bib27) 2018; 18 Kohlenberg, Monnet, Plachouras (bib32) 2022; 27 Calvo, Melo, Perozo-Mena (bib6) 2016; 73 Schelenz, Hagen, Rhodes (bib7) 2016; 5 Muñoz, Welsh, Shea (bib19) 2021; 218 Welsh, Bentz, Shams (bib8) 2017; 55 Chowdhary, Prakash, Sharma (bib25) 2018; 73 Eyre, Sheppard, Madder (bib4) 2018; 379 Lockhart, Etienne, Vallabhaneni (bib12) 2017; 64 Kwon, Shin, Byun (bib28) 2019; 57 Treangen, Ondov, Koren, Phillippy (bib17) 2014; 15 (bib10) 2019 de Groot, Spruijtenburg, Parnell, Chow, Meis (bib23) 2022; 10 Li, Muñoz, Gade (bib18) 2023; 9 Wang, Xu (bib22) 2022; 20 Spruijtenburg, Badali, Abastabar (bib11) 2022; 11 Kilburn, Innes, Quinn (bib31) 2022; 66 Tan, Teo, Rahman (bib15) 2019; 54 Muñoz, Gade, Chow (bib24) 2018; 9 Ruiz-Gaitán, Moret, Tasias-Pitarch (bib2) 2018; 61 Lyman, Forsberg, Sexton (bib30) 2023; 176 Iguchi, Itakura, Yoshida (bib14) 2019; 25 Theodoropoulos, Bolstorff, Bozorgzadeh (bib5) 2020; 20 Chow, de Groot, Badali, Abastabar, Chiller, Meis (bib13) 2019; 25 Spruijtenburg, Bombassaro, Meijer (bib20) 2023; 86 Yingtaweesittikul, Ko, Abdul Rahman, Tan, Nagarajan, Suphavilai (bib35) 2021; 8 Ko, Chu, Lim (bib33) 2022; 9 Ben Abid, Salah, Sundararaju (bib29) 2023; 29 Ko, Chng, Nagarajan (bib34) 2022; 7 Alexander, Novembre, Lange (bib21) 2009; 19 Morales-López, Parra-Giraldo, Ceballos-Garzón (bib1) 2017; 23 Muñoz (10.1016/S2666-5247(24)00101-0_bib24) 2018; 9 Tan (10.1016/S2666-5247(24)00101-0_bib15) 2019; 54 Hernando-Ortiz (10.1016/S2666-5247(24)00101-0_bib16) 2021; 12 Wang (10.1016/S2666-5247(24)00101-0_bib22) 2022; 20 Eyre (10.1016/S2666-5247(24)00101-0_bib4) 2018; 379 de Groot (10.1016/S2666-5247(24)00101-0_bib23) 2022; 10 Ben Abid (10.1016/S2666-5247(24)00101-0_bib29) 2023; 29 Chow (10.1016/S2666-5247(24)00101-0_bib27) 2018; 18 Chow (10.1016/S2666-5247(24)00101-0_bib26) 2020; 11 Kilburn (10.1016/S2666-5247(24)00101-0_bib31) 2022; 66 Lockhart (10.1016/S2666-5247(24)00101-0_bib12) 2017; 64 Li (10.1016/S2666-5247(24)00101-0_bib18) 2023; 9 Ko (10.1016/S2666-5247(24)00101-0_bib34) 2022; 7 Spruijtenburg (10.1016/S2666-5247(24)00101-0_bib11) 2022; 11 Calvo (10.1016/S2666-5247(24)00101-0_bib6) 2016; 73 Iguchi (10.1016/S2666-5247(24)00101-0_bib14) 2019; 25 Theodoropoulos (10.1016/S2666-5247(24)00101-0_bib5) 2020; 20 Chowdhary (10.1016/S2666-5247(24)00101-0_bib25) 2018; 73 Muñoz (10.1016/S2666-5247(24)00101-0_bib19) 2021; 218 Kwon (10.1016/S2666-5247(24)00101-0_bib28) 2019; 57 Treangen (10.1016/S2666-5247(24)00101-0_bib17) 2014; 15 Yingtaweesittikul (10.1016/S2666-5247(24)00101-0_bib35) 2021; 8 Kohlenberg (10.1016/S2666-5247(24)00101-0_bib32) 2022; 27 Morales-López (10.1016/S2666-5247(24)00101-0_bib1) 2017; 23 Adams (10.1016/S2666-5247(24)00101-0_bib3) 2018; 24 Welsh (10.1016/S2666-5247(24)00101-0_bib8) 2017; 55 Chow (10.1016/S2666-5247(24)00101-0_bib13) 2019; 25 Schelenz (10.1016/S2666-5247(24)00101-0_bib7) 2016; 5 Fisher (10.1016/S2666-5247(24)00101-0_bib9) 2023; 21 Lyman (10.1016/S2666-5247(24)00101-0_bib30) 2023; 176 Ruiz-Gaitán (10.1016/S2666-5247(24)00101-0_bib2) 2018; 61 Alexander (10.1016/S2666-5247(24)00101-0_bib21) 2009; 19 Spruijtenburg (10.1016/S2666-5247(24)00101-0_bib20) 2023; 86 Ko (10.1016/S2666-5247(24)00101-0_bib33) 2022; 9 |
References_xml | – volume: 20 start-page: 3673 year: 2020 end-page: 3679 ident: bib5 article-title: outbreak involving liver transplant recipients in a surgical intensive care unit publication-title: Am J Transplant contributor: fullname: Bozorgzadeh – volume: 11 year: 2020 ident: bib26 article-title: Tracing the evolutionary history and global expansion of publication-title: MBio contributor: fullname: Gade – volume: 24 start-page: 1816 year: 2018 end-page: 1824 ident: bib3 article-title: in healthcare facilities, New York, USA, 2013–2017 publication-title: Emerg Infect Dis contributor: fullname: Tsay – volume: 73 start-page: 891 year: 2018 end-page: 899 ident: bib25 article-title: A multicentre study of antifungal susceptibility patterns among 350 publication-title: J Antimicrob Chemother contributor: fullname: Sharma – volume: 64 start-page: 134 year: 2017 end-page: 140 ident: bib12 article-title: Simultaneous emergence of multidrug-resistant publication-title: Clin Infect Dis contributor: fullname: Vallabhaneni – volume: 21 start-page: 211 year: 2023 end-page: 212 ident: bib9 article-title: The WHO fungal priority pathogens list as a game-changer publication-title: Nat Rev Microbiol contributor: fullname: Denning – volume: 9 start-page: 5346 year: 2018 ident: bib24 article-title: Genomic insights into multidrug-resistance, mating and virulence in publication-title: Nat Commun contributor: fullname: Chow – volume: 27 year: 2022 ident: bib32 article-title: Increasing number of cases and outbreaks caused by publication-title: Euro Surveill contributor: fullname: Plachouras – volume: 54 start-page: 709 year: 2019 end-page: 715 ident: bib15 article-title: in Singapore: genomic epidemiology, antifungal drug resistance, and identification using the updated 8.01 VITEK publication-title: Int J Antimicrob Agents contributor: fullname: Rahman – volume: 57 year: 2019 ident: bib28 article-title: clinical isolates from South Korea: identification, antifungal susceptibility, and genotyping publication-title: J Clin Microbiol contributor: fullname: Byun – volume: 379 start-page: 1322 year: 2018 end-page: 1331 ident: bib4 article-title: A publication-title: N Engl J Med contributor: fullname: Madder – volume: 18 start-page: 1377 year: 2018 end-page: 1384 ident: bib27 article-title: Multiple introductions and subsequent transmission of multidrug-resistant publication-title: Lancet Infect Dis contributor: fullname: Tsay – volume: 23 start-page: 162 year: 2017 end-page: 164 ident: bib1 article-title: Invasive infections with multidrug-resistant yeast publication-title: Emerg Infect Dis contributor: fullname: Ceballos-Garzón – volume: 176 start-page: 489 year: 2023 end-page: 495 ident: bib30 article-title: Worsening spread of publication-title: Ann Intern Med contributor: fullname: Sexton – volume: 55 start-page: 2996 year: 2017 end-page: 3005 ident: bib8 article-title: Survival, persistence, and isolation of the emerging multidrug-resistant pathogenic yeast publication-title: J Clin Microbiol contributor: fullname: Shams – volume: 86 start-page: 610 year: 2023 end-page: 613 ident: bib20 article-title: genotyping reveals numerous independent zoonotic introductions in Brazil publication-title: J Infect contributor: fullname: Meijer – volume: 9 year: 2022 ident: bib33 article-title: Clonal serotype 1c multidrug-resistant publication-title: Front Med (Lausanne) contributor: fullname: Lim – year: 2019 ident: bib10 article-title: Antibiotic Resistance Coordination and Strategy Unit. Antibiotic resistance threats in the United States – volume: 7 start-page: 486 year: 2022 end-page: 496 ident: bib34 article-title: Metagenomics-enabled microbial surveillance publication-title: Nat Microbiol contributor: fullname: Nagarajan – volume: 61 start-page: 498 year: 2018 end-page: 505 ident: bib2 article-title: An outbreak due to publication-title: Mycoses contributor: fullname: Tasias-Pitarch – volume: 12 start-page: 1063 year: 2021 end-page: 1075 ident: bib16 article-title: Virulence of publication-title: Virulence contributor: fullname: Eraso – volume: 5 start-page: 35 year: 2016 ident: bib7 article-title: First hospital outbreak of the globally emerging publication-title: Antimicrob Resist Infect Control contributor: fullname: Rhodes – volume: 73 start-page: 369 year: 2016 end-page: 374 ident: bib6 article-title: First report of publication-title: J Infect contributor: fullname: Perozo-Mena – volume: 19 start-page: 1655 year: 2009 end-page: 1664 ident: bib21 article-title: Fast model-based estimation of ancestry in unrelated individuals publication-title: Genome Res contributor: fullname: Lange – volume: 9 year: 2023 ident: bib18 article-title: Comparing genomic variant identification protocols for publication-title: Microb Genom contributor: fullname: Gade – volume: 20 start-page: 3030 year: 2022 end-page: 3040 ident: bib22 article-title: Population genomic analyses reveal evidence for limited recombination in the superbug publication-title: Comput Struct Biotechnol J contributor: fullname: Xu – volume: 25 start-page: 1780 year: 2019 end-page: 1781 ident: bib13 article-title: Potential fifth clade of publication-title: Emerg Infect Dis contributor: fullname: Meis – volume: 10 year: 2022 ident: bib23 article-title: Optimization and validation of publication-title: Microbiol Spectr contributor: fullname: Meis – volume: 29 start-page: 1083.e1 year: 2023 end-page: 1083.e7 ident: bib29 article-title: Molecular characterization of publication-title: Clin Microbiol Infect contributor: fullname: Sundararaju – volume: 66 year: 2022 ident: bib31 article-title: Antifungal resistance trends of publication-title: Antimicrob Agents Chemother contributor: fullname: Quinn – volume: 15 start-page: 524 year: 2014 ident: bib17 article-title: The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes publication-title: Genome Biol contributor: fullname: Phillippy – volume: 11 start-page: 2405 year: 2022 end-page: 2411 ident: bib11 article-title: Confirmation of fifth publication-title: Emerg Microbes Infect contributor: fullname: Abastabar – volume: 8 year: 2021 ident: bib35 article-title: CalmBelt: rapid SARS-CoV-2 genome characterization for outbreak tracking publication-title: Front Med (Lausanne) contributor: fullname: Suphavilai – volume: 218 year: 2021 ident: bib19 article-title: Clade-specific chromosomal rearrangements and loss of subtelomeric adhesins in publication-title: Genetics contributor: fullname: Shea – volume: 25 start-page: 743 year: 2019 end-page: 749 ident: bib14 article-title: : a pathogen difficult to identify, treat, and eradicate and its characteristics in Japanese strains publication-title: J Infect Chemother contributor: fullname: Yoshida – volume: 57 year: 2019 ident: 10.1016/S2666-5247(24)00101-0_bib28 article-title: Candida auris clinical isolates from South Korea: identification, antifungal susceptibility, and genotyping publication-title: J Clin Microbiol doi: 10.1128/JCM.01624-18 contributor: fullname: Kwon – volume: 8 year: 2021 ident: 10.1016/S2666-5247(24)00101-0_bib35 article-title: CalmBelt: rapid SARS-CoV-2 genome characterization for outbreak tracking publication-title: Front Med (Lausanne) contributor: fullname: Yingtaweesittikul – volume: 64 start-page: 134 year: 2017 ident: 10.1016/S2666-5247(24)00101-0_bib12 article-title: Simultaneous emergence of multidrug-resistant Candida auris on 3 continents confirmed by whole-genome sequencing and epidemiological analyses publication-title: Clin Infect Dis doi: 10.1093/cid/ciw691 contributor: fullname: Lockhart – volume: 20 start-page: 3030 year: 2022 ident: 10.1016/S2666-5247(24)00101-0_bib22 article-title: Population genomic analyses reveal evidence for limited recombination in the superbug Candida auris in nature publication-title: Comput Struct Biotechnol J doi: 10.1016/j.csbj.2022.06.030 contributor: fullname: Wang – volume: 23 start-page: 162 year: 2017 ident: 10.1016/S2666-5247(24)00101-0_bib1 article-title: Invasive infections with multidrug-resistant yeast Candida auris, Colombia publication-title: Emerg Infect Dis doi: 10.3201/eid2301.161497 contributor: fullname: Morales-López – volume: 20 start-page: 3673 year: 2020 ident: 10.1016/S2666-5247(24)00101-0_bib5 article-title: Candida auris outbreak involving liver transplant recipients in a surgical intensive care unit publication-title: Am J Transplant doi: 10.1111/ajt.16144 contributor: fullname: Theodoropoulos – volume: 15 start-page: 524 year: 2014 ident: 10.1016/S2666-5247(24)00101-0_bib17 article-title: The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes publication-title: Genome Biol doi: 10.1186/s13059-014-0524-x contributor: fullname: Treangen – volume: 12 start-page: 1063 year: 2021 ident: 10.1016/S2666-5247(24)00101-0_bib16 article-title: Virulence of Candida auris from different clinical origins in Caenorhabditis elegans and Galleria mellonella host models publication-title: Virulence doi: 10.1080/21505594.2021.1908765 contributor: fullname: Hernando-Ortiz – volume: 5 start-page: 35 year: 2016 ident: 10.1016/S2666-5247(24)00101-0_bib7 article-title: First hospital outbreak of the globally emerging Candida auris in a European hospital publication-title: Antimicrob Resist Infect Control doi: 10.1186/s13756-016-0132-5 contributor: fullname: Schelenz – volume: 73 start-page: 369 year: 2016 ident: 10.1016/S2666-5247(24)00101-0_bib6 article-title: First report of Candida auris in America: clinical and microbiological aspects of 18 episodes of candidemia publication-title: J Infect contributor: fullname: Calvo – volume: 19 start-page: 1655 year: 2009 ident: 10.1016/S2666-5247(24)00101-0_bib21 article-title: Fast model-based estimation of ancestry in unrelated individuals publication-title: Genome Res doi: 10.1101/gr.094052.109 contributor: fullname: Alexander – volume: 25 start-page: 1780 year: 2019 ident: 10.1016/S2666-5247(24)00101-0_bib13 article-title: Potential fifth clade of Candida auris, Iran, 2018 publication-title: Emerg Infect Dis doi: 10.3201/eid2509.190686 contributor: fullname: Chow – volume: 10 year: 2022 ident: 10.1016/S2666-5247(24)00101-0_bib23 article-title: Optimization and validation of Candida auris short tandem repeat analysis publication-title: Microbiol Spectr doi: 10.1128/spectrum.02645-22 contributor: fullname: de Groot – volume: 86 start-page: 610 year: 2023 ident: 10.1016/S2666-5247(24)00101-0_bib20 article-title: Sporothrix brasiliensis genotyping reveals numerous independent zoonotic introductions in Brazil publication-title: J Infect contributor: fullname: Spruijtenburg – volume: 176 start-page: 489 year: 2023 ident: 10.1016/S2666-5247(24)00101-0_bib30 article-title: Worsening spread of Candida auris in the United States, 2019 to 2021 publication-title: Ann Intern Med doi: 10.7326/M22-3469 contributor: fullname: Lyman – volume: 9 year: 2022 ident: 10.1016/S2666-5247(24)00101-0_bib33 article-title: Clonal serotype 1c multidrug-resistant Shigella flexneri detected in multiple institutions by sentinel-site sequencing publication-title: Front Med (Lausanne) contributor: fullname: Ko – volume: 18 start-page: 1377 year: 2018 ident: 10.1016/S2666-5247(24)00101-0_bib27 article-title: Multiple introductions and subsequent transmission of multidrug-resistant Candida auris in the USA: a molecular epidemiological survey publication-title: Lancet Infect Dis doi: 10.1016/S1473-3099(18)30597-8 contributor: fullname: Chow – volume: 29 start-page: 1083.e1 year: 2023 ident: 10.1016/S2666-5247(24)00101-0_bib29 article-title: Molecular characterization of Candida auris outbreak isolates in Qatar from patients with COVID-19 reveals the emergence of isolates resistant to three classes of antifungal drugs publication-title: Clin Microbiol Infect doi: 10.1016/j.cmi.2023.04.025 contributor: fullname: Ben Abid – volume: 66 year: 2022 ident: 10.1016/S2666-5247(24)00101-0_bib31 article-title: Antifungal resistance trends of Candida auris clinical isolates in New York and New Jersey from 2016 to 2020 publication-title: Antimicrob Agents Chemother doi: 10.1128/aac.02242-21 contributor: fullname: Kilburn – volume: 379 start-page: 1322 year: 2018 ident: 10.1016/S2666-5247(24)00101-0_bib4 article-title: A Candida auris outbreak and its control in an intensive care setting publication-title: N Engl J Med doi: 10.1056/NEJMoa1714373 contributor: fullname: Eyre – volume: 7 start-page: 486 year: 2022 ident: 10.1016/S2666-5247(24)00101-0_bib34 article-title: Metagenomics-enabled microbial surveillance publication-title: Nat Microbiol doi: 10.1038/s41564-022-01089-w contributor: fullname: Ko – volume: 54 start-page: 709 year: 2019 ident: 10.1016/S2666-5247(24)00101-0_bib15 article-title: Candida auris in Singapore: genomic epidemiology, antifungal drug resistance, and identification using the updated 8.01 VITEKⓇ2 system publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2019.09.016 contributor: fullname: Tan – volume: 218 year: 2021 ident: 10.1016/S2666-5247(24)00101-0_bib19 article-title: Clade-specific chromosomal rearrangements and loss of subtelomeric adhesins in Candida auris publication-title: Genetics doi: 10.1093/genetics/iyab029 contributor: fullname: Muñoz – volume: 25 start-page: 743 year: 2019 ident: 10.1016/S2666-5247(24)00101-0_bib14 article-title: Candida auris: a pathogen difficult to identify, treat, and eradicate and its characteristics in Japanese strains publication-title: J Infect Chemother doi: 10.1016/j.jiac.2019.05.034 contributor: fullname: Iguchi – volume: 9 year: 2023 ident: 10.1016/S2666-5247(24)00101-0_bib18 article-title: Comparing genomic variant identification protocols for Candida auris publication-title: Microb Genom contributor: fullname: Li – volume: 73 start-page: 891 year: 2018 ident: 10.1016/S2666-5247(24)00101-0_bib25 article-title: A multicentre study of antifungal susceptibility patterns among 350 Candida auris isolates (2009–17) in India: role of the ERG11 and FKS1 genes in azole and echinocandin resistance publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkx480 contributor: fullname: Chowdhary – volume: 27 year: 2022 ident: 10.1016/S2666-5247(24)00101-0_bib32 article-title: Increasing number of cases and outbreaks caused by Candida auris in the EU/EEA, 2020 to 2021 publication-title: Euro Surveill doi: 10.2807/1560-7917.ES.2022.27.46.2200846 contributor: fullname: Kohlenberg – volume: 21 start-page: 211 year: 2023 ident: 10.1016/S2666-5247(24)00101-0_bib9 article-title: The WHO fungal priority pathogens list as a game-changer publication-title: Nat Rev Microbiol doi: 10.1038/s41579-023-00861-x contributor: fullname: Fisher – volume: 11 year: 2020 ident: 10.1016/S2666-5247(24)00101-0_bib26 article-title: Tracing the evolutionary history and global expansion of Candida auris using population genomic analyses publication-title: MBio doi: 10.1128/mBio.03364-19 contributor: fullname: Chow – volume: 55 start-page: 2996 year: 2017 ident: 10.1016/S2666-5247(24)00101-0_bib8 article-title: Survival, persistence, and isolation of the emerging multidrug-resistant pathogenic yeast Candida auris on a plastic health care surface publication-title: J Clin Microbiol doi: 10.1128/JCM.00921-17 contributor: fullname: Welsh – volume: 61 start-page: 498 year: 2018 ident: 10.1016/S2666-5247(24)00101-0_bib2 article-title: An outbreak due to Candida auris with prolonged colonisation and candidaemia in a tertiary care European hospital publication-title: Mycoses doi: 10.1111/myc.12781 contributor: fullname: Ruiz-Gaitán – volume: 11 start-page: 2405 year: 2022 ident: 10.1016/S2666-5247(24)00101-0_bib11 article-title: Confirmation of fifth Candida auris clade by whole genome sequencing publication-title: Emerg Microbes Infect doi: 10.1080/22221751.2022.2125349 contributor: fullname: Spruijtenburg – volume: 24 start-page: 1816 year: 2018 ident: 10.1016/S2666-5247(24)00101-0_bib3 article-title: Candida auris in healthcare facilities, New York, USA, 2013–2017 publication-title: Emerg Infect Dis doi: 10.3201/eid2410.180649 contributor: fullname: Adams – volume: 9 start-page: 5346 year: 2018 ident: 10.1016/S2666-5247(24)00101-0_bib24 article-title: Genomic insights into multidrug-resistance, mating and virulence in Candida auris and related emerging species publication-title: Nat Commun doi: 10.1038/s41467-018-07779-6 contributor: fullname: Muñoz |
SSID | ssj0002512155 |
Score | 2.390387 |
Snippet | The emerging fungal pathogen Candida auris poses a serious threat to global public health due to its worldwide distribution, multidrug resistance, high... |
SourceID | proquest crossref pubmed elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 100878 |
Title | Detection and characterisation of a sixth Candida auris clade in Singapore: a genomic and phenotypic study |
URI | https://dx.doi.org/10.1016/S2666-5247(24)00101-0 https://www.ncbi.nlm.nih.gov/pubmed/39008997 https://www.proquest.com/docview/3081288815 |
Volume | 5 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bi9QwGA26ouyLeHe8LBEUlKFjp0kv8U3Wy-Kwvuwu7FtI0hS77LbF6aDjg7_dL0mbDOrg5aXMNG1acg7p-ZLvgtBTXWqa5LGIJHzOI0qJigQrVKQzmqucaREr6yD7MTs4oR9O09ONiGsTXdLLmfr227iS_0EVzgGuJkr2H5D1ncIJ-A34whEQhuNfYfxG93oo9W2i00LuZa8DxXRZf7VhRk1Zl2Jqkggtp-pclDZZyJEpiN0ZV1sb82wStl4MCVyN71fbrzv4G1LQngVuueDpmfG7NxFFYXOpM3H_567K9f4nsTb9h63-dohAAwt9uvgCk8ui9j5BrrDzwmQWWgX_YLdCe6jr6ftaby5SJNR7YcE3xk5moAMyMHpdds1fpm23gnDkLwJxndBnCbMZ8KJ48x4Yj-7C4kmgGazFPHzJvH_h2HQZXTEJEk1NhcPvYe3NaDrQUSGg62V49POEvhgeu4uujR1tUy3brBKrTo5voOuDWYFfO47cRJd0cwtddYVG17fRmWcKBmzxz0zBbYUFtkzBA1OwZQq2TMF1gz1TXsGVA09sX4En2PLkDjp59_Z4_yAaqmxEKqGkj6jMGBMloQWtzC58rLJMCgXSVWhJQM2ReRYnYNWLhCpWxoVURKVEMKmrjBWS3EU7Tdvo-wineVmRUjIxlzllpCoqIvJU5pKlsqhyMkGzcRR555KpcO9laBDgBgGeUG4R4PEEFeNY80EROqXHgTl_uvXJiA2HGdNsg4lGt6slJ6CCk6Io5ukE3XOg-bcZ8X6wteUh2g0Ef4R2-s8r_Rh0aS_37HrOnqXaD9jNiCE |
link.rule.ids | 314,780,784,864,27924,27925 |
linkProvider | ISSN International Centre |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Detection+and+characterisation+of+a+sixth+Candida+auris+clade+in+Singapore%3A+a+genomic+and+phenotypic+study&rft.jtitle=The+Lancet.+Microbe&rft.au=Suphavilai%2C+Chayaporn&rft.au=Ko%2C+Karrie+Kwan+Ki&rft.au=Lim%2C+Kar+Mun&rft.au=Tan%2C+Mei+Gie&rft.date=2024-09-01&rft.eissn=2666-5247&rft_id=info:doi/10.1016%2FS2666-5247%2824%2900101-0&rft_id=info%3Apmid%2F39008997&rft.externalDocID=39008997 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2666-5247&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2666-5247&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2666-5247&client=summon |