Spectroscopic Investigation on the Interaction of a Cyanine Dye with Serum Albumins
The interactions of a cyanine dye with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by using absorption and fluorescence spectra. Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a n...
Saved in:
| Published in | Chinese journal of chemistry Vol. 26; no. 2; pp. 397 - 401 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Weinheim
WILEY-VCH Verlag
01.02.2008
WILEY‐VCH Verlag |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | The interactions of a cyanine dye with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by using absorption and fluorescence spectra. Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a notable intensity change. Furthermore, the number of binding sites (n) was identified by the absorption spectra. There is a constant enhancement of fluorescence quantum yield when the cyanine dye complexes with HSA or BSA. The apparent binding constant (Ka) and the free energy changes (ΔG) were obtained by analysis of fluorescence data of the cyanine dye in the absence and presence of HSA and BSA. Compared to BSA, HSA associates with the dye in a stronger way. |
|---|---|
| AbstractList | The interactions of a cyanine dye with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by using absorption and fluorescence spectra. Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a notable intensity change. Furthermore, the number of binding sites (
n
) was identified by the absorption spectra. There is a constant enhancement of fluorescence quantum yield when the cyanine dye complexes with HSA or BSA. The apparent binding constant (
K
a
) and the free energy changes (Δ
G
) were obtained by analysis of fluorescence data of the cyanine dye in the absence and presence of HSA and BSA. Compared to BSA, HSA associates with the dye in a stronger way. The interactions of a cyanine dye with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by using absorption and fluorescence spectra. Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a notable intensity change. Furthermore, the number of binding sites (n) was identified by the absorption spectra. There is a constant enhancement of fluorescence quantum yield when the cyanine dye complexes with HSA or BSA. The apparent binding constant (Ka) and the free energy changes (ΔG) were obtained by analysis of fluorescence data of the cyanine dye in the absence and presence of HSA and BSA. Compared to BSA, HSA associates with the dye in a stronger way. |
| Author | ZHANG, Ya-Zhou YAN, Wen-Peng YANG, Qian-Fan XU, Guang-Zhi TANG, Ya-Lin DU, Hong-Yan |
| Author_xml | – sequence: 1 givenname: Ya-Zhou surname: ZHANG fullname: ZHANG, Ya-Zhou organization: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China – sequence: 2 givenname: Qian-Fan surname: YANG fullname: YANG, Qian-Fan organization: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China – sequence: 3 givenname: Hong-Yan surname: DU fullname: DU, Hong-Yan organization: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China – sequence: 4 givenname: Ya-Lin surname: TANG fullname: TANG, Ya-Lin email: zyansong@hotmail.com organization: E-mail: zyansong@hotmail.com – sequence: 5 givenname: Guang-Zhi surname: XU fullname: XU, Guang-Zhi organization: Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China – sequence: 6 givenname: Wen-Peng surname: YAN fullname: YAN, Wen-Peng organization: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100080, China |
| BookMark | eNqFkE1Pg0AQhjemJrbVq2f-AHV2gQWODdW2htgDGr1tlmWwWyk0sLXy76ViGmNiTCaZyZt55uMdkUFZlUjINYUJBWA3alOpCQMIQgCfn5Eh5dS1feDeoKsBqM3Bfbkgo6bZdP2-z_iQJMkOlamrRlU7raxl-Y6N0a_S6Kq0ujBr7ESDtVS9lFvSilpZ6hKtWYvWQZu1lWC931rTIt1vddlckvNcFg1efecxebq7fYwWdryaL6NpbCvmMm6nqe-FXDqc-gHjIVCaSRo6DiBmCpB7LnMBUsoyjysIVJqzwMuQygC5Qkc5YzLp56ru_qbGXOxqvZV1KyiIoyXiaIk4WdIB7i9AafP1qqmlLv7Gwh476ALbf5aI6H4V_WTtntWNwY8TK-s3wX3H98Tzw1zEcQAzSBaCOZ_P5If5 |
| CitedBy_id | crossref_primary_10_1002_slct_201902327 crossref_primary_10_1016_j_jphotobiol_2008_11_013 crossref_primary_10_3390_pharmaceutics14051056 crossref_primary_10_1016_j_dyepig_2012_09_007 crossref_primary_10_1016_j_saa_2011_10_052 crossref_primary_10_1021_jo500657s crossref_primary_10_1016_j_dyepig_2017_01_015 crossref_primary_10_1088_1742_6596_704_1_012012 crossref_primary_10_1134_S0012501615050036 crossref_primary_10_1002_cjoc_201500733 crossref_primary_10_1016_j_talanta_2012_01_029 crossref_primary_10_1016_j_optmat_2024_116043 crossref_primary_10_1016_j_carbpol_2016_12_068 crossref_primary_10_1039_D1FO00487E crossref_primary_10_1002_cjoc_200890252 crossref_primary_10_1039_C7MB00185A crossref_primary_10_1016_j_jphotobiol_2011_11_002 crossref_primary_10_3390_chemosensors10080314 crossref_primary_10_1016_j_jphotochem_2020_112710 crossref_primary_10_1002_cjoc_201090196 crossref_primary_10_1088_1742_6596_924_1_012006 crossref_primary_10_1021_acsomega_4c07997 |
| Cites_doi | 10.1002/9780470186794.ch6 10.1016/S0065-3233(08)60065-0 10.1111/j.1751-1097.2005.tb00202.x 10.1016/S0301-4622(03)00218-7 10.1021/ja973812c 10.1021/ja01176a030 10.1007/s00216-007-1153-y 10.1016/j.ab.2006.11.029 10.1038/358209a0 10.1002/1097-0134(20010501)43:2<75::AID-PROT1019>3.0.CO;2-7 10.1021/j100782a050 10.1002/bip.10489 10.1021/ja984279j 10.1021/jp971472b 10.1002/1522-2683(200107)22:12<2512::AID-ELPS2512>3.0.CO;2-9 |
| ContentType | Journal Article |
| Copyright | Copyright © 2008 SIOC, CAS, Shanghai & WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
| Copyright_xml | – notice: Copyright © 2008 SIOC, CAS, Shanghai & WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
| DBID | BSCLL AAYXX CITATION |
| DOI | 10.1002/cjoc.200890076 |
| DatabaseName | Istex CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | CrossRef |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry |
| EISSN | 1614-7065 |
| EndPage | 401 |
| ExternalDocumentID | 10_1002_cjoc_200890076 CJOC200890076 ark_67375_WNG_LL80D0SH_2 |
| Genre | shortCommunication |
| GroupedDBID | -SB -S~ .3N .GA .Y3 05W 0R~ 10A 1L6 1OB 1OC 29B 31~ 33P 3SF 3WU 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5GY 5VR 5VS 5XA 5XC 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM AAESR AAEVG AAHHS AANLZ AAONW AASGY AAXDM AAXRX AAZKR ABCQN ABCUV ABDBF ABEML ABIJN ABJNI ABPVW ACAHQ ACBWZ ACCFJ ACCZN ACGFS ACIWK ACPOU ACSCC ACXBN ACXQS ADBBV ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN ADZOD AEEZP AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFFPM AFGKR AFPWT AFRAH AFUIB AHBTC AITYG AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ATUGU AUFTA AZBYB AZFZN AZVAB BAFTC BDRZF BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BSCLL BY8 BZXJU CAJEB CCEZO CDRFL CHBEP CS3 CW9 D-E D-F DCZOG DPXWK DR2 DRFUL DRSTM EBS EJD F00 F01 F04 FA0 FEDTE G-S G.N GODZA H.T H.X HF~ HGLYW HVGLF HZ~ IX1 J0M JPC LATKE LAW LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ O66 O9- OIG P2W P4D PALCI Q-- Q.N Q11 QB0 QRW R.K RIWAO RJQFR RK2 RNS ROL RWI RX1 RYL SAMSI SUPJJ U1G U5L W8V W99 WBFHL WBKPD WIH WIK WOHZO WQJ WRC WXSBR WYISQ XG1 XV2 ZZTAW ~IA ~WT AAHQN AAMNL AANHP AAYCA ACRPL ACUHS ACYXJ ADNMO AFWVQ ALVPJ AAYXX AEYWJ AGHNM AGQPQ AGYGG CITATION TGP |
| ID | FETCH-LOGICAL-c2426-bb7596a36178269011da19330eedc0e6542400b12d56c08cbf285de1a8e6ce3c3 |
| IEDL.DBID | DR2 |
| ISSN | 1001-604X |
| IngestDate | Tue Jul 01 03:34:50 EDT 2025 Thu Apr 24 22:59:16 EDT 2025 Wed Jan 22 16:19:33 EST 2025 Wed Oct 30 09:59:46 EDT 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Language | English |
| License | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c2426-bb7596a36178269011da19330eedc0e6542400b12d56c08cbf285de1a8e6ce3c3 |
| Notes | istex:EAD2BD2F5CFF503C2FA3CA4CA6319097E6197E9D ArticleID:CJOC200890076 ark:/67375/WNG-LL80D0SH-2 Tel.: 027‐87543232; Fax: 086‐027‐87543632 |
| PageCount | 5 |
| ParticipantIDs | crossref_primary_10_1002_cjoc_200890076 crossref_citationtrail_10_1002_cjoc_200890076 wiley_primary_10_1002_cjoc_200890076_CJOC200890076 istex_primary_ark_67375_WNG_LL80D0SH_2 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2008-02 February, 2008 2008-02-00 |
| PublicationDateYYYYMMDD | 2008-02-01 |
| PublicationDate_xml | – month: 02 year: 2008 text: 2008-02 |
| PublicationDecade | 2000 |
| PublicationPlace | Weinheim |
| PublicationPlace_xml | – name: Weinheim |
| PublicationTitle | Chinese journal of chemistry |
| PublicationTitleAlternate | Chin. J. Chem |
| PublicationYear | 2008 |
| Publisher | WILEY-VCH Verlag WILEY‐VCH Verlag |
| Publisher_xml | – name: WILEY-VCH Verlag – name: WILEY‐VCH Verlag |
| References | Sengupta, B.; Sengupta, P. K.. Biopolymers, 2003, 72, 427. Guharay, G.; Sengupta, B.; Sengupta, P. K.. Proteins: Struct., Funct., Genet., 2001, 43, 75. Owen, D. J.; VanDerveer, D.; Schuster, G. B.. J. Am. Chem. Soc., 1998, 120, 1705. Ficken, G. E., In The Chemistry of Synthetic Dyes, Vol. 4, Ed.: Venkataraman, K., Academic Press, New York, 1971, pp. 228-240. Peters, T.. Adv. Protein Chem., 1985, 37, 161. Seifert, J. L.; Connor, R. E.; Kushon, S. A.; Wang, M.; Armitage, B. A.. J. Am. Chem. Soc., 1999, 121, 2987. Ina, G. P.; Natalia, P. S.; Svetlana, B. D.; Rimma, A. P.; Gennady, T. S.; Alexander, S. T.. Anal. Biochem., 2007, 361, 183. Hamer, F. M., In The Chemistry of Heterocyclic Compounds, Vol. 18, Ed.: Weissberger, A., Interscence, New York, 1964, pp. 148-200. Sowell, J.; Mason, J. C.; Strekowski, L.; Patonay, G.. Electrophoresis, 2001, 22, 2512. Benesi, H. A.; Hildebrand, J. H.. J. Am. Chem. Soc., 1949, 71, 2703. Bean, R. C.; Stepherd, W. C.; Kay, R. E.; Walwick, E. R.. J. Phys. Chem., 1965, 69, 4368. Tatikolov, A. S.; Costa, S. M. B.. Biophys. Chem., 2004, 107, 33. Khimenko, V.; Chibisov, A. K.; Gorner, H.. J. Phys. Chem. A, 1997, 101, 7304. Brown, J. R.; Shockly, P., In Lipid-protein Interactions, Vol. 1, Eds.: Jost, P. C.; Griffith, O. H., John Wiley, New York, 1982, pp. 25-68. Wang, H.; Li, W.; Guo, X.; Zhang, H.. Anal. Bioanal. Chem., 2007, 387, 2857. He, X. M.; Carter, D. C.. Nature, 1992, 358, 209. Mallick, A.; Chattopadhyay, N.. Photochem. Photobiol., 2005, 81, 419. 2007; 387 1965; 69 1982; 1 1964; 18 2007; 361 1997; 101 1992; 358 1999; 121 2005; 81 2001; 22 2003; 72 2004; 107 1949; 71 1985; 37 1998; 120 1971; 4 2001; 43 Ficken G. E. (e_1_2_1_14_2) 1971 Brown J. R. (e_1_2_1_3_2) 1982 e_1_2_1_6_2 e_1_2_1_7_2 e_1_2_1_4_2 e_1_2_1_5_2 e_1_2_1_2_2 e_1_2_1_11_2 e_1_2_1_12_2 e_1_2_1_10_2 e_1_2_1_15_2 e_1_2_1_16_2 e_1_2_1_13_2 e_1_2_1_8_2 e_1_2_1_17_2 e_1_2_1_9_2 e_1_2_1_18_2 |
| References_xml | – reference: Ficken, G. E., In The Chemistry of Synthetic Dyes, Vol. 4, Ed.: Venkataraman, K., Academic Press, New York, 1971, pp. 228-240. – reference: Sowell, J.; Mason, J. C.; Strekowski, L.; Patonay, G.. Electrophoresis, 2001, 22, 2512. – reference: Tatikolov, A. S.; Costa, S. M. B.. Biophys. Chem., 2004, 107, 33. – reference: Guharay, G.; Sengupta, B.; Sengupta, P. K.. Proteins: Struct., Funct., Genet., 2001, 43, 75. – reference: Owen, D. J.; VanDerveer, D.; Schuster, G. B.. J. Am. Chem. Soc., 1998, 120, 1705. – reference: Peters, T.. Adv. Protein Chem., 1985, 37, 161. – reference: Ina, G. P.; Natalia, P. S.; Svetlana, B. D.; Rimma, A. P.; Gennady, T. S.; Alexander, S. T.. Anal. Biochem., 2007, 361, 183. – reference: Mallick, A.; Chattopadhyay, N.. Photochem. Photobiol., 2005, 81, 419. – reference: Khimenko, V.; Chibisov, A. K.; Gorner, H.. J. Phys. Chem. A, 1997, 101, 7304. – reference: Seifert, J. L.; Connor, R. E.; Kushon, S. A.; Wang, M.; Armitage, B. A.. J. Am. Chem. Soc., 1999, 121, 2987. – reference: Bean, R. C.; Stepherd, W. C.; Kay, R. E.; Walwick, E. R.. J. Phys. Chem., 1965, 69, 4368. – reference: Wang, H.; Li, W.; Guo, X.; Zhang, H.. Anal. Bioanal. Chem., 2007, 387, 2857. – reference: He, X. M.; Carter, D. C.. Nature, 1992, 358, 209. – reference: Hamer, F. M., In The Chemistry of Heterocyclic Compounds, Vol. 18, Ed.: Weissberger, A., Interscence, New York, 1964, pp. 148-200. – reference: Brown, J. R.; Shockly, P., In Lipid-protein Interactions, Vol. 1, Eds.: Jost, P. C.; Griffith, O. H., John Wiley, New York, 1982, pp. 25-68. – reference: Sengupta, B.; Sengupta, P. K.. Biopolymers, 2003, 72, 427. – reference: Benesi, H. A.; Hildebrand, J. H.. J. Am. Chem. Soc., 1949, 71, 2703. – volume: 361 start-page: 183 year: 2007 publication-title: Anal. Biochem. – volume: 69 start-page: 4368 year: 1965 publication-title: J. Phys. Chem. – volume: 37 start-page: 161 year: 1985 publication-title: Adv. Protein Chem. – volume: 387 start-page: 2857 year: 2007 publication-title: Anal. Bioanal. Chem. – volume: 358 start-page: 209 year: 1992 publication-title: Nature – volume: 43 start-page: 75 year: 2001 publication-title: Proteins: Struct., Funct., Genet. – volume: 107 start-page: 33 year: 2004 publication-title: Biophys. Chem. – volume: 1 start-page: 25 year: 1982 end-page: 68 – volume: 121 start-page: 2987 year: 1999 publication-title: J. Am. Chem. Soc. – volume: 22 start-page: 2512 year: 2001 publication-title: Electrophoresis – volume: 18 start-page: 148 year: 1964 end-page: 200 – volume: 4 start-page: 228 year: 1971 end-page: 240 – volume: 120 start-page: 1705 year: 1998 publication-title: J. Am. Chem. Soc. – volume: 72 start-page: 427 year: 2003 publication-title: Biopolymers – volume: 101 start-page: 7304 year: 1997 publication-title: J. Phys. Chem. A – volume: 81 start-page: 419 year: 2005 publication-title: Photochem. Photobiol. – volume: 71 start-page: 2703 year: 1949 publication-title: J. Am. Chem. Soc. – ident: e_1_2_1_13_2 doi: 10.1002/9780470186794.ch6 – ident: e_1_2_1_2_2 doi: 10.1016/S0065-3233(08)60065-0 – ident: e_1_2_1_7_2 doi: 10.1111/j.1751-1097.2005.tb00202.x – ident: e_1_2_1_17_2 doi: 10.1016/S0301-4622(03)00218-7 – ident: e_1_2_1_12_2 doi: 10.1021/ja973812c – ident: e_1_2_1_18_2 doi: 10.1021/ja01176a030 – ident: e_1_2_1_11_2 doi: 10.1007/s00216-007-1153-y – ident: e_1_2_1_10_2 doi: 10.1016/j.ab.2006.11.029 – ident: e_1_2_1_4_2 doi: 10.1038/358209a0 – start-page: 25 volume-title: Lipid‐protein Interactions year: 1982 ident: e_1_2_1_3_2 – ident: e_1_2_1_6_2 doi: 10.1002/1097-0134(20010501)43:2<75::AID-PROT1019>3.0.CO;2-7 – ident: e_1_2_1_16_2 doi: 10.1021/j100782a050 – start-page: 228 volume-title: The Chemistry of Synthetic Dyes year: 1971 ident: e_1_2_1_14_2 – ident: e_1_2_1_5_2 doi: 10.1002/bip.10489 – ident: e_1_2_1_8_2 doi: 10.1021/ja984279j – ident: e_1_2_1_9_2 doi: 10.1021/jp971472b – ident: e_1_2_1_15_2 doi: 10.1002/1522-2683(200107)22:12<2512::AID-ELPS2512>3.0.CO;2-9 |
| SSID | ssj0027726 |
| Score | 1.8445337 |
| Snippet | The interactions of a cyanine dye with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by using absorption and fluorescence... |
| SourceID | crossref wiley istex |
| SourceType | Enrichment Source Index Database Publisher |
| StartPage | 397 |
| SubjectTerms | bovine serum albumin cyanine dye fluorescent probe human serum albumin |
| Title | Spectroscopic Investigation on the Interaction of a Cyanine Dye with Serum Albumins |
| URI | https://api.istex.fr/ark:/67375/WNG-LL80D0SH-2/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fcjoc.200890076 |
| Volume | 26 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3dS8MwEA8yH_TFb3F-kQfRp279Sps-SuccY05wDvdWkjSDOW3HPsD515tL124TRFDoSyEp6SW5u1zufj-ErrhP-0TywICTlwEescFduOSNGbPMQPqUQXHyQ9trdN1mj_RWqvgzfIgi4AY7Q-tr2OCMT6pL0FDxmmoIQhrAbZJSwpCwBV7Rk708cfmabw1whgzPdHs5aqNpV9e7r1mlTRDwx7q3qs1NfRexfKBZlsmwMpvyivj8huH4nz_ZQzsLXxTfZotnH23I5ABthTkF3CHqADv9FPAu09FA4BVMjjTB6lHOI9Yhxaw6Aqd9zHA4Z4kaEq7NJYYoL1bqaPaO9V3AIJkcoW797jlsGAsWBkOA-TY490ngMQdqCW2gr7JiZkEYRFlXYUogvFJ6gFt2TDxhUsH7NiWxtBiVnpCOcI5RKUkTeYIwdR2PM1Mtm5gCrA0DfyL244AQRgLKysjIZyESC4hyYMp4izJwZTsCUUWFqMropmg_ysA5fmx5rSe1aMbGQ0hp80n00r6PWi1q1sxOI7LLyNZT9cv3orD5GBZvp3_pdIa2s9wTSI05R6XpeCYvlIMz5Zd6EX8B0wLwxQ |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1JS8QwFH64HPTiLo5rDqKnjl0mbXqUjlq1jqAOegtJmgG3VnQG1F9vXzqtjiCCQi-FpKTJS97LW74PYFsGrEe1DC28eVloEVuyhUHeVAjHDnXABBYnn3X8uNs6uaFVNiHWwpT4ELXDDXeGOa9xg6NDeu8TNVTd5QaDkIUYThqHSaT1Rvj89oX7eecKDOMaIg1Zvt26qXAbbXdvtP-IXprEKX4dtVeNwjmcBVkNtcwzuW8O-rKp3r-hOP7rX-ZgZmiOkv1SfuZhTGcLMBVVLHCLcIkE9X2EvMyfbhX5AsuRZ6R4CvuRGK9iWSBB8h4RJHoTWTEm0n7TBB29pDiRBo_EhANus5cl6B4eXEWxNSRisBRqcEvKgIa-8LCc0EUGKycVDnpCCgWrbI2cV8VRIB03pb6ymZI9l9FUO4JpX2lPecswkeWZXgHCWp4vhV1ITsoQ2UagSZEGaUipoCETDbCqZeBqiFKOZBkPvMRXdjlOFa-nqgG7dfunEp_jx5Y7ZlXrZuL5HrPaAsqvO0c8SZjdti9j7jbANWv1y_d4dHIe1W-rf-m0BVPx1VnCk-PO6RpMl6komCmzDhP954HeKOydvtw0Ev0B8fv04Q |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LT-MwEB4BlRYuPHZZUZ4-IDgFnDROnCNKKQVKWS2L6M2yHVfqAkkFrQT8ejJOm1IkhARSLpHsyLHHnvE8vg9gV4W8y4yKHLx5OWgRO8rHIG8ipUsjE3KJxckX7aB57Z91WOdNFX-BD1E63HBn2PMaN3g_6R5OQEP1_8xCEPIIo0mzUPEDGiF5Q_2vN7lyhZZwDYGGnID6nTFsI_UOp_tPqaUKzvDTtLlq9U1jCeR4pEWaye3BcKAO9Ms7EMfv_MoyLI6MUXJUSM8KzJj0J8zHYw64X3CF9PQDBLzM-j1N3oByZCnJn9x6JNanWJRHkKxLJImfZZoPidSfDUE3L8nPo-E9scGAXvq4CteN439x0xnRMDga9bejVMiiQNawmNBD_io3kS76QXL1qqlBxqv8IFCul7BAU65V1-MsMa7kJtCmpmu_YS7NUrMGhPu1QEmay03CEddGokGRhEnEmGQRl1Vwxqsg9AijHKky7kSBruwJnCpRTlUV9sv2_QKd48OWe3ZRy2by4RZz2kImbtonotXitE6vmsKrgmeX6pPvifjsMi7f1r_SaQd-_Kk3ROu0fb4BC0UeCqbJbMLc4GFotnJjZ6C2rTy_AhMK85A |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Spectroscopic+Investigation+on+the+Interaction+of+a+Cyanine+Dye+with+Serum+Albumins&rft.jtitle=Chinese+journal+of+chemistry&rft.au=ZHANG%2C+Ya%E2%80%90Zhou&rft.au=YANG%2C+Qian%E2%80%90Fan&rft.au=DU%2C+Hong%E2%80%90Yan&rft.au=TANG%2C+Ya%E2%80%90Lin&rft.date=2008-02-01&rft.pub=WILEY%E2%80%90VCH+Verlag&rft.issn=1001-604X&rft.eissn=1614-7065&rft.volume=26&rft.issue=2&rft.spage=397&rft.epage=401&rft_id=info:doi/10.1002%2Fcjoc.200890076&rft.externalDBID=10.1002%252Fcjoc.200890076&rft.externalDocID=CJOC200890076 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1001-604X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1001-604X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1001-604X&client=summon |