pH-dependent binding of ATP aptamer to the target and competition strands: Fluorescent melting curve fitting study

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 11; p. 130689
• Main Authors: Gabrusenok, P.V., Ramazanov, R.R., Kasyanenko, N.A., Lantushenko, A.O., Sokolov, P.A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2024
• Subjects: Aptamer; ATP; Fluorescent; Melting curve analysis; Molecular switch; Thermodynamics; Thermodynamics; Molecular switch; pH; Fluorescent; Model; Melting curve analysis; Aptamer; ATP

The pH varies in different tissues and organelles and also changes during some diseases. In this regard, the application of molecular switches that use a competition-based aptamer switch design in biological systems requires studying the thermodynamics of such systems at different pH values. In this...