Expression of Rhizopus Glucoamylase Gene in Yeast and Its Application

Rhizopus glucoamylase gene was cloned, sequenced and expressed in yeast. After improvement of the promotors, yeast strains, vectors and carbon sources, upto 13U/ml of glucoamylase was secreted. This means that about 8% of the total yeast protein was secreted as glucoamylase. The study of the structu...

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Published inDenpun kagaku = Journal of the Japanese Society of Starch Science Vol. 34; no. 2; pp. 148 - 154
Main Authors NAKAMURA, Norihisa, KUNISAKI, Shin-ichi, KIUCHI, Naoko, SHIBANO, Yuji, TANAKA, Yoshikazu, YOSHIZUMI, Hajime, ASHIKARI, Toshihiko
Format Journal Article
LanguageEnglish
Published The Japanese Society of Applied Glycoscience 1987
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ISSN0021-5406
1884-488X
DOI10.5458/jag1972.34.148

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Summary:Rhizopus glucoamylase gene was cloned, sequenced and expressed in yeast. After improvement of the promotors, yeast strains, vectors and carbon sources, upto 13U/ml of glucoamylase was secreted. This means that about 8% of the total yeast protein was secreted as glucoamylase. The study of the structure and function relationship of this glucoamylase suggested that the glucoamylase consisted of two domains; adsorptive domain and catalytic domain. By using the resultant recombinant yeast strain, alcoholic fermentation of starchy materials was also achieved.
ISSN:0021-5406
1884-488X
DOI:10.5458/jag1972.34.148