Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter

The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity...

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Published inRussian journal of bioorganic chemistry Vol. 41; no. 5; pp. 525 - 528
Main Authors Pereverzev, A. P., Matlashov, M. E., Staroverov, D. B., Lukyanov, K. A., Gurskaya, N. G.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.09.2015
Subjects
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Life Sciences
Organic Chemistry
fluorescent protein
genetically encoded sensor
NMD
cell culture
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Abstract The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity in single live cells from the ratio of signals of the green and red fluorescent proteins. The following cell lines were analyzed: mouse colon carcinoma CT26, mouse Lewis lung carcinoma LLC, human T-cell leukemia Jurkat, and spontaneously immortalized human keratinocytes HaCaT. These cell lines demonstrated very different NMD activities. The NMD activity in the CT26 cells was low, while in the LLC cells—high (8.5-fold higher than in CT26). The Jurkat and HaCaT cells demonstrated high heterogeneity and included two subpopulations of cells with low and high NMD activities. In addition, high NMD activity was demonstrated for the primary culture of mouse embryonic hippocampal neurons.
AbstractList The activity of nonsense-mediated mRNA decay (NMD) cascade in several mammalian cell lines was investigated. The analysis was conducted with the help of the recently developed genetically encoded fluorescent sensor [Pereverzev et al.]. This fluorescent NMD-reporter allows estimating the NMD activity in single live cells from the ratio of signals of the green and red fluorescent proteins. The following cell lines were analyzed: mouse colon carcinoma CT26, mouse Lewis lung carcinoma LLC, human T-cell leukemia Jurkat, and spontaneously immortalized human keratinocytes HaCaT. These cell lines demonstrated very different NMD activities. The NMD activity in the CT26 cells was low, while in the LLC cells—high (8.5-fold higher than in CT26). The Jurkat and HaCaT cells demonstrated high heterogeneity and included two subpopulations of cells with low and high NMD activities. In addition, high NMD activity was demonstrated for the primary culture of mouse embryonic hippocampal neurons.
Author Matlashov, M. E.
Staroverov, D. B.
Pereverzev, A. P.
Gurskaya, N. G.
Lukyanov, K. A.
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cell culture
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Title Differences in nonsense-mediated mRNA decay activity in mammalian cell lines revealed by a fluorescence reporter
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